Microbiome Profile and Molecular Pathways Alterations in Gastrointestinal Tract by Hydrogen Sulfide-Releasing Nonsteroidal Anti-Inflammatory Drug (ATB-352): Insight into Possible Safer Polypharmacy

Głowacka, Urszula; Magierowska, Katarzyna; Wójcik, Dagmara; Hankus, Jerzy; Szetela, Małgorzata; Cieszkowski, Jakub; Korbut, Edyta; Danielak, Aleksandra; Surmiak, Marcin; Chmura, Anna; Wallace, John L.; Magierowski, Marcin

doi:10.1089/ars.2020.8240

Cited by 13 publications

(12 citation statements)

References 67 publications

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“…DNA was isolated from stool samples using QIAamp DNA Stool Mini Kits (Qiagen, Hilden, Germany) according to the manufacturers' protocol, as described previously [10]. An evaluation of bacterial population was performed based on the analysis of the hypervariable V3-V4 region of 16S ribosomal RNA (rRNA) [17,65]. For the region amplification and library preparation, specific sequences of 341F and 785R primers were used (16S analysis).…”

Section: Next Generation Sequencing Of Gut Microbiomementioning

confidence: 99%

Alkaline Phosphatase Relieves Colitis in Obese Mice Subjected to Forced Exercise via Its Anti-Inflammatory and Intestinal Microbiota-Shaping Properties

Wojcik-Grzybek,

Sliwowski,

Kwiecien

et al. 2024

IJMS

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Intestinal alkaline phosphatase (IAP) is an enzyme that plays a protective role in the gut. This study investigated the effect of IAP treatment on experimental colitis in mice subjected to forced exercise on a high-fat diet. C57BL/6 mice with TNBS colitis were fed a high-fat diet and subjected to forced treadmill exercise with or without IAP treatment. Disease activity, oxidative stress, inflammatory cytokines, and gut microbiota were assessed. Forced exercise exacerbated colitis in obese mice, as evidenced by increased disease activity index (DAI), oxidative stress markers, and proinflammatory adipokines and cytokines. IAP treatment significantly reduced these effects and promoted the expression of barrier proteins in the colonic mucosa. Additionally, IAP treatment altered the gut microbiota composition, favoring beneficial Verrucomicrobiota and reducing pathogenic Clostridia and Odoribacter. IAP treatment ameliorates the worsening effect of forced exercise on murine colitis by attenuating oxidative stress, downregulating proinflammatory biomarkers, and modulating the gut microbiota. IAP warrants further investigation as a potential therapeutic strategy for ulcerative colitis.

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Section: Next Generation Sequencing Of Gut Microbiomementioning

confidence: 99%

Alkaline Phosphatase Relieves Colitis in Obese Mice Subjected to Forced Exercise via Its Anti-Inflammatory and Intestinal Microbiota-Shaping Properties

Wojcik-Grzybek,

Sliwowski,

Kwiecien

et al. 2024

IJMS

Self Cite

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show abstract

“…The cons of NSAIDs are gastrointestinal complications, hepatotoxic problems, renal injury, cardiovascular problems, cerebral complications, respiratory tract issues, and mitochondrial toxicity ( 72 ). Future perspectives in designing new NSAIDs are to decrease gastrointestinal complications by adding –NO and –H2S groups or donors, like EV-34 ( 73 ), ATB-352 ( 74 ), and ATB-346 (NCT03291418, NCT03978208, NCT03220633) ( 75 , 76 ). Another challenge in designing new NSAIDs is to find highly selective inhibitors of the COX-2 enzyme to increase the benefit/risk profile ( 77 – 80 ).…”

Section: Classic Pharmacological Approach For Immunomodulationmentioning

confidence: 99%

Immunomodulation—a general review of the current state-of-the-art and new therapeutic strategies for targeting the immune system

Strzelec

Detka²,

Mieszczak³

et al. 2023

Front. Immunol.

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In recent years, there has been a tremendous development of biotechnological, pharmacological, and medical techniques which can be implemented in the functional modulation of the immune system components. Immunomodulation has attracted much attention because it offers direct applications in both basic research and clinical therapy. Modulation of a non-adequate, amplified immune response enables to attenuate the clinical course of a disease and restore homeostasis. The potential targets to modulate immunity are as multiple as the components of the immune system, thus creating various possibilities for intervention. However, immunomodulation faces new challenges to design safer and more efficacious therapeutic compounds. This review offers a cross-sectional picture of the currently used and newest pharmacological interventions, genomic editing, and tools for regenerative medicine involving immunomodulation. We reviewed currently available experimental and clinical evidence to prove the efficiency, safety, and feasibility of immunomodulation in vitro and in vivo. We also reviewed the advantages and limitations of the described techniques. Despite its limitations, immunomodulation is considered as therapy itself or as an adjunct with promising results and developing potential.

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“…In comparison, in the same model, the H 2 S-releasing donor compound ATB-352) significantly reduced GI tract damage via the preservation of H 2 S levels and suppression of oxidative/inflammatory response pathways in treated animals. It was noted that ATB-352 had an improved safety profile and preserved GI mucosal integrity by inhibiting inflammation in tissues [ 91 ].…”

Section: Introductionmentioning

confidence: 99%

The Impact of Drugs on Hydrogen Sulfide Homeostasis in Mammals

et al. 2023

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Mammalian cells and tissues have the capacity to generate hydrogen sulfide gas (H2S) via catabolic routes involving cysteine metabolism. H2S acts on cell signaling cascades that are necessary in many biochemical and physiological roles important in the heart, brain, liver, kidney, urogenital tract, and cardiovascular and immune systems of mammals. Diminished levels of this molecule are observed in several pathophysiological conditions including heart disease, diabetes, obesity, and immune function. Interestingly, in the last two decades, it has become apparent that some commonly prescribed pharmacological drugs can impact the expression and activities of enzymes responsible for hydrogen sulfide production in cells and tissues. Therefore, the current review provides an overview of the studies that catalogue key drugs and their impact on hydrogen sulfide production in mammals.

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Microbiome Profile and Molecular Pathways Alterations in Gastrointestinal Tract by Hydrogen Sulfide-Releasing Nonsteroidal Anti-Inflammatory Drug (ATB-352): Insight into Possible Safer Polypharmacy

Cited by 13 publications

References 67 publications

Alkaline Phosphatase Relieves Colitis in Obese Mice Subjected to Forced Exercise via Its Anti-Inflammatory and Intestinal Microbiota-Shaping Properties

Alkaline Phosphatase Relieves Colitis in Obese Mice Subjected to Forced Exercise via Its Anti-Inflammatory and Intestinal Microbiota-Shaping Properties

Immunomodulation—a general review of the current state-of-the-art and new therapeutic strategies for targeting the immune system

The Impact of Drugs on Hydrogen Sulfide Homeostasis in Mammals

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