Microbiosensors for glucose based on Prussian Blue modified carbon fiber electrodes for in vivo monitoring in the central nervous system

Salazar, Pedro; Martín, Miriam; Roche, R.; González–Mora, José Luis; O’Neill, Robert

doi:10.1016/j.bios.2010.06.045

Cited by 41 publications

(25 citation statements)

References 40 publications

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“…gold-coated screen-printed electrode as peroxide transducer for GOx [46] 0.68 up to 0.6 carbon fiber (250 mm length, 8 mm diameter) modified with peroxide transducer and GOx [47] 9.3 0.1-2 carbon fiber (200-500 mm length, 7 mm diameter) modified with GOx mediated by MnO2 [48] 22.7 1.5-15 carbon fiber (500 mm length, 30 mm diameter) modified with ruthenium as peroxide transducer for immobilized GOx [49] 20.5 0-4 this work 77 1-30…”

Section: Discussionmentioning

confidence: 99%

Arrays of Screen‐Printed Graphite Microband Electrodes as a Versatile Electroanalysis Platform

et al. 2014

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Arrays of microband electrodes were developed by screen printing followed by cutting, which enabled the realization of microband arrays at the cut edge. Microband arrays of different designs were characterised with physical and electrochemical methods. In both cases the methods showed that the microband width was around 5 m. Semi-steady-state cyclic voltammetry responses were observed for redox probes.Chronocoulometric measurements showed the establishment of convergent diffusion regimes characterized by current densities similar to those of a single microelectrode.The analytical performance of the elaborated electrode system and its versatility were illustrated with two electrochemical assays: detection of ascorbic acid via direct oxidation and a mediated glucose biosensor fabricated via dip coating. Due to convergent mass transport both systems showed enhancement of the analytical characteristics. The developed approach can be adapted to automated electrode recovery.

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Section: Discussionmentioning

confidence: 99%

Arrays of Screen‐Printed Graphite Microband Electrodes as a Versatile Electroanalysis Platform

et al. 2014

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“…In this way, the biosensor was cycled in the presence of 1 mm glucose from 25 to 1000 mV s À1 ( Figure 6A). [73] [72] Figure 7 shows the chronoamperograms and the calibration curves obtained for 0.0 to 16 mm glucose (in phosphate buffer solution (PBS), pH 7.4, E app = À0.10 V) by using our nonoptimized DPR-700/Fe 3 O 4 @pDA150/Gox configuration.…”

Section: Ir Spectroscopymentioning

confidence: 99%

“…Data showed a linear dependence at low glucose concentrations and saturation at high concentrations, in agreement with the Michaelis-Menten kinetics. [73]…”

Section: Ir Spectroscopymentioning

confidence: 99%

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Quinone‐Rich Poly(dopamine) Magnetic Nanoparticles for Biosensor Applications

et al. 2014

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Novel core-shell quinone-rich poly(dopamine)-magnetic nanoparticles (MNPs) were prepared by using an in situ polymerization method. Catechol groups were oxidized to quinone by using a thermal treatment. MNPs were characterized by using X-ray diffraction, X-ray photoelectron spectroscopy, atomic force microscopy, magnetic force microscopy, UV/Vis, Fourier-transform infrared spectroscopy, and electrochemical techniques. The hybrid nanomaterial showed an average core diameter of 17 nm and a polymer-film thickness of 2 nm. The core-shell nanoparticles showed high reactivity and were used as solid supports for the covalent immobilization of glucose oxidase (Gox) through Schiff base formation and Michael addition. The amount of Gox immobilized onto the nanoparticle surface was almost twice that of the nonoxidized film. The resulting biofunctionalized MNPs were used to construct an amperometric biosensor for glucose. The enzyme biosensor has a sensitivity of 8.7 mA M(-1) cm(-2) , a low limit of detection (0.02 mM), and high stability for 45 days. Finally, the biosensor was used to determine glucose in blood samples and was checked against a commercial glucometer.

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“…At these potentials, however, endogenous reducing agents, such as ascorbic acid (AA), which is present in high concentrations in most biological tissues and fluids, can give rise to false positive biosensor responses in vivo . A permselective barrier is therefore essential in the design of implantable biosensors, and a range of electro-deposited poly-phenylenediamines [30,31,32,33,34,35] and polyphenols [36,37,38,39] have been developed to improve biosensor selectivity for brain monitoring where ECF ascorbate levels are particularly high (up to 1 mM) [40,41]. …”

Section: Introductionmentioning

confidence: 99%

Characterization of Biosensors Based on Recombinant Glutamate Oxidase: Comparison of Crosslinking Agents in Terms of Enzyme Loading and Efficiency Parameters

Ford

Quinn

O’Neill

2016

Sensors

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Amperometric l-glutamate (Glu) biosensors, based on both wild-type and a recombinant form of l-glutamate oxidase (GluOx), were designed and characterized in terms of enzyme-kinetic, sensitivity and stability parameters in attempts to fabricate a real-time Glu monitoring device suitable for future long-term detection of this amino acid in biological and other complex media. A comparison of the enzyme from these two sources showed that they were similar in terms of biosensor performance. Optimization of the loading of the polycationic stabilization agent, polyethyleneimine (PEI), was established before investigating a range of crosslinking agents under different conditions: glutaraldehyde (GA), polyethylene glycol (PEG), and polyethylene glycol diglycidyl ether (PEGDE). Whereas PEI-free biosensor designs lost most of their meager Glu sensitivity after one or two days, configurations with a 2:5 ratio of dip-evaporation applications of PEI(1%):GluOx(400 U/mL) displayed a 20-fold increase in their initial sensitivity, and a decay half-life extended to 10 days. All the crosslinkers studied had no effect on initial Glu sensitivity, but enhanced biosensor stability, provided the crosslinking procedure was carried out under well-defined conditions. The resulting biosensor design based on the recombinant enzyme deposited on a permselective layer of poly-(ortho-phenylenediamine), PoPD/PEI2/GluOx5/PEGDE, displayed good sensitivity (LOD < 0.2 μM), response time (t90% < 1 s) and stability over a 90-day period, making it an attractive candidate for future long-term monitoring of Glu concentration dynamics in complex media.

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Microbiosensors for glucose based on Prussian Blue modified carbon fiber electrodes for in vivo monitoring in the central nervous system

Cited by 41 publications

References 40 publications

Arrays of Screen‐Printed Graphite Microband Electrodes as a Versatile Electroanalysis Platform

Arrays of Screen‐Printed Graphite Microband Electrodes as a Versatile Electroanalysis Platform

Quinone‐Rich Poly(dopamine) Magnetic Nanoparticles for Biosensor Applications

Characterization of Biosensors Based on Recombinant Glutamate Oxidase: Comparison of Crosslinking Agents in Terms of Enzyme Loading and Efficiency Parameters

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