Microbodies in Experimentally Altered Cells

Reddy, Janardan K.; Chiga, Masahiro; Bunyaratvej, S; Svoboda, Donald J.

doi:10.1083/jcb.44.1.226

Cited by 33 publications

(4 citation statements)

References 21 publications

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“…How ever, the effect of hydroxydione is not readily explained. Reddy et al, 1970; Svoboda el aI., 1969]. In our experim ents we also could not find an unequivocal relation between the microbody population of liver cells and the biologic effects of the compounds used.…”

contrasting

confidence: 36%

Relationship between microbodies and the endoplasmic reticulum in the liver of adult rats

Horváth¹,

Kovács²,

Blascheck³

1971

Cells Tissues Organs

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contrasting

confidence: 36%

Relationship between microbodies and the endoplasmic reticulum in the liver of adult rats

Horváth¹,

Kovács²,

Blascheck³

1971

Cells Tissues Organs

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“…(44) to suggest 10 yr ago that increase of the catalase-deficient peroxisome population might be caused by possible induction of other unidentified peroxisomal components. Although the peroxisomes induced in the presence of allylisopropylacetamide (a potent inhibitor of catalase synthesis) were deficient in catalase activity, they nevertheless exerted a substantial serum lipid-lowering effect in these rats fed peroxisome proliferators (45) .…”

Section: Discussionmentioning

confidence: 99%

Immunochemical identity of peroxisomal enoyl-CoA hydratase with the peroxisome-proliferation -associated 80,000 mol wt polypeptide in rat liver

Reddy¹,

Qureshi²,

Hollenberg³

et al. 1981

The Journal of Cell Biology

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Peroxisome proliferators, which induce proliferation of hepatic peroxisomes, have been shown previously to cause a marked increase in an 80,000 mol wt polypeptide predominantly in the light mitochondrial and microsomal fractions of liver of rodents. We now present evidence to show that this hepatic peroxisome-proliferation-associated polypeptide, referred to as polypeptide PPA-80, is immunochemically identical with the mutifunctional peroxisome protein displaying heat-labile enoyl-CoA hydratase activity . This conclusion is based on the following observations : (a) the purified polypeptide PPA-80 and the heat-labile enoyl-CoA hydratase from livers of rats treated with the peroxisome proliferator Wy-14,643 ([4-chloro-6(2,3-xylidino)-2-pyrimidinylthio]acetic acid) exhibit identical minimum molecular weights of 80,000 on SDS polyacrylamide gel electrophoresis; (b) these two proteins are immunochemically identical on the basis of Ouchterlony double diffusion, immunotitration, rocket immunoelectrophoresis, and crossed immunoelectrophoresis analyses ; and (c) the immunoprecipitates formed by antibodies to polypeptide PPA-80 when dissociated on a Sephadex G-200 column yield enoyl-CoA hydratase activity . Whether the polypeptide PPA-80 exhibits the activity of other enzyme(s) of the peroxisomal Q-oxidation system such as fatty acyl-CoA oxidase activity or displays immunochemical identity with such enzymes remains to be determined .The availability of antibodies to polypeptide PPA-80 and enoyl-CoA hydratase facilitated immunofluorescent and immunocytochemical localization of the polypeptide PPA-80 and enoyl-CoA hydratase in the rat liver. The indirect immunofluorescent studies with these antibodies provided direct visual evidence for the marked induction of polypeptide PPA-80 and enoyl-CoA hydratase in the livers of rats treated with Wy-14,643. The present studies also provide immunocytochemical evidence for the localization of polypeptide PPA-80 and the heat-labile enoyl-CoA hydratase in the peroxisome, but not in the mitochondria, of hepatic parenchymal cells. These studies, therefore, provide morphological evidence for the existence of fatty acyl-CoA oxidizing system in peroxisomes . An increase of polypeptide PPA-80 on SDS polyacrylamide gel electrophoretic analysis of the subcellular fractions of liver of rodents treated with lipid-lowering drugs should serve as a reliable and sensitive indicator of enhanced peroxisomal #-oxidation system . Several structurally unrelated hypolipidemic drugs and certain industrial plasticizers such as di-(2-ethylhexyl)phthalate which cause profound proliferation of peroxisomes in hepatic parenchymal cells of rodents (37,38,50,51), also cause a marked increase in the quantity of a polypeptide with an approximate 406

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“…The waves of synthesis might be explained by a synchronization of DNA synthesis. Reddy et al (1969) reported large increases in [3H]thymidine incorporation and mitotic activity after a single injection of thioacetamide (50mg/kg body weight). If a large proportion of the cells became synchronized in their synthesis of DNA after ingestion of thioacetamide a biphasic incorporation of [3H]thymidine would result.…”

Section: Adolescent Ratsmentioning

confidence: 99%

Studies of nuclei separated by zonal centrifugation from liver of rats treated with thioacetamide

González-Mujica

Mathias

1973

Biochemical Journal

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1. The effects of the inclusion of thioacetamide in the diet on the properties of rat liver nuclei were studied both in adolescent rats, in which the parenchymal cells contain diploid nuclei, and in young adult rats, with a high proportion of tetraploid nuclei. 2. These investigations included a survey of the sedimentation properties of the nuclei, the nuclear volumes, content of DNA, RNA and protein, the incorporation in vivo of [(3)H]thymidine into DNA and [(14)C]orotate into RNA, and measurements of the activity of RNA polymerase and ribonuclease. These studies were conducted on nuclei fractionated by zonal centrifugation. 3. In both groups of animals, exposure to thioacetamide produced large numbers of nuclei that were abnormal in their chemical composition and enzymic activity. The changes were complex as regards both the types of nuclei that were affected and in their variation with time. 4. In adolescent rats two waves of synthesis of DNA and RNA were observed, one at 3 days and the other after 2 weeks of treatment. The first decline in the incorporations into both DNA and RNA coincided with a decrease in the pool sizes of some of the precursors. The activity of RNA polymerase was not substantially altered. A marked increase in the content of protein was observed before the first wave of synthesis. The normal progressive increase in tetraploid nuclei was prevented. 5. In young adult rats two waves of DNA synthesis were detected. Each was preceded by a large increase in the amount of protein per nucleus but was not accompanied by increased RNA synthesis. After 4 weeks of treatment, the diploid stromal nuclei appeared mainly unaffected and large numbers of tetraploid nuclei with a greatly increased quantity of protein were observed.

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Microbodies in Experimentally Altered Cells

Cited by 33 publications

References 21 publications

Relationship between microbodies and the endoplasmic reticulum in the liver of adult rats

Relationship between microbodies and the endoplasmic reticulum in the liver of adult rats

Immunochemical identity of peroxisomal enoyl-CoA hydratase with the peroxisome-proliferation -associated 80,000 mol wt polypeptide in rat liver

Studies of nuclei separated by zonal centrifugation from liver of rats treated with thioacetamide

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