Microbubble Agents: New Directions

Stride, Eleanor; Segers, Tim; Lajoinie, Guillaume; Cherkaoui, Samir; Bettinger, Thierry; Versluis, Michel; Borden, Mark A.

doi:10.1016/j.ultrasmedbio.2020.01.027

Cited by 138 publications

(116 citation statements)

References 157 publications

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“…As one can see, all bubbles have a mean size of 1-2 µm with a small size dispersion, and the use of dialysis led to a slight decrease (~0.1-0. All the described samples had a mean size corresponding to the criteria for contrast agents (they were less than 10 μm) [52,53], and a relatively small size dispersion for bubbles prepared by the sonication method, which confirmed the selected conditions for agents' preparation and functionalization. Thus, characterization of imaging properties is needed to determine the potential biomedical applications for such probes.…”

Section: Samples Characterization: Concentration and Mean Size Of Stamentioning

confidence: 53%

Air-Filled Bubbles Stabilized by Gold Nanoparticle/Photodynamic Dye Hybrid Structures for Theranostics

et al. 2021

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Microbubbles have already reached clinical practice as ultrasound contrast agents for angiography. However, modification of the bubbles’ shell is needed to produce probes for ultrasound and multimodal (fluorescence/photoacoustic) imaging methods in combination with theranostics (diagnostics and therapeutics). In the present work, hybrid structures based on microbubbles with an air core and a shell composed of bovine serum albumin, albumin-coated gold nanoparticles, and clinically available photodynamic dyes (zinc phthalocyanine, indocyanine green) were shown to achieve multimodal imaging for potential applications in photodynamic therapy. Microbubbles with an average size of 1.5 ± 0.3 μm and concentration up to 1.2 × 109 microbubbles/mL were obtained and characterized. The introduction of the dye into the system reduced the solution’s surface tension, leading to an increase in the concentration and stability of bubbles. The combination of gold nanoparticles and photodynamic dyes’ influence on the fluorescent signal and probes’ stability is described. The potential use of the obtained probes in biomedical applications was evaluated using fluorescence tomography, raster-scanning optoacoustic microscopy and ultrasound response measurements using a medical ultrasound device at the frequency of 33 MHz. The results demonstrate the impact of microbubbles’ stabilization using gold nanoparticle/photodynamic dye hybrid structures to achieve probe applications in theranostics.

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Section: Samples Characterization: Concentration and Mean Size Of Stamentioning

confidence: 53%

Air-Filled Bubbles Stabilized by Gold Nanoparticle/Photodynamic Dye Hybrid Structures for Theranostics

et al. 2021

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“…In the last decades, in vivo imaging devices have become fundamental tools in basic sciences, in preclinical research and in modern drug development to visualize nanocomposites. The most suitable and commonly used techniques are magnetic resonance imaging (MRI), optical imaging (OI), positron emission tomography (PET), 15 computed tomography and ultrasonography, 16 and a number of recent articles focused on the visualization of nanoconstructs by these approaches in a biological environment.…”

Section: Imaging Techniques Applied To In Vivo Modelsmentioning

confidence: 99%

Imaging techniques in nanomedical research

Calderan

Malatesta

2020

Eur J Histochem

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About twenty years ago, nanotechnology began to be applied to biomedical issues giving rise to the research field called nanomedicine. Thus, the study of the interactions between nanomaterials and the biological environment became of primary importance in order to design safe and effective nanoconstructs suitable for diagnostic and/or therapeutic purposes. Consequently, imaging techniques have increasingly been used in the production, characterisation and preclinical/clinical application of nanomedical tools. This work aims at making an overview of the microscopy and imaging techniques in vivo and in vitro in their application to nanomedical investigation, and to stress their contribution to this developing research field.

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“…To stabilize the gas core, microbubbles are usually coated with a phospholipid monolayer, proteins, or polymers. For a schematic representation, the reader is referred to recent reviews on microbubbles [5,6]. The coating reduces surface tension and gas diffusion [7].…”

Section: Introductionmentioning

confidence: 99%

The Impact of Lipid Handling and Phase Distribution on the Acoustic Behavior of Microbubbles

et al. 2021

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Phospholipid-coated microbubbles are ultrasound contrast agents that can be employed for ultrasound molecular imaging and drug delivery. For safe and effective implementation, microbubbles must respond uniformly and predictably to ultrasound. Therefore, we investigated how lipid handling and phase distribution affected the variability in the acoustic behavior of microbubbles. Cholesterol was used to modify the lateral molecular packing of 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC)-based microbubbles. To assess the effect of lipid handling, microbubbles were produced by a direct method, i.e., lipids directly dispersed in an aqueous medium or indirect method, i.e., lipids first dissolved in an organic solvent. The lipid phase and ligand distribution in the microbubble coating were investigated using confocal microscopy, and the acoustic response was recorded with the Brandaris 128 ultra-high-speed camera. In microbubbles with 12 mol% cholesterol, the lipids were miscible and all in the same phase, which resulted in more buckle formation, lower shell elasticity and higher shell viscosity. Indirect DSPC microbubbles had a more uniform response to ultrasound than direct DSPC and indirect DSPC-cholesterol microbubbles. The difference in lipid handling between direct and indirect DSPC microbubbles significantly affected the acoustic behavior. Indirect DSPC microbubbles are the most promising candidate for ultrasound molecular imaging and drug delivery applications.

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Microbubble Agents: New Directions

Cited by 138 publications

References 157 publications

Air-Filled Bubbles Stabilized by Gold Nanoparticle/Photodynamic Dye Hybrid Structures for Theranostics

Air-Filled Bubbles Stabilized by Gold Nanoparticle/Photodynamic Dye Hybrid Structures for Theranostics

Imaging techniques in nanomedical research

The Impact of Lipid Handling and Phase Distribution on the Acoustic Behavior of Microbubbles

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