2014
DOI: 10.1016/j.snb.2014.03.023
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Microcantilever array biosensors for detection and recognition of Gram-negative bacterial endotoxins

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Cited by 23 publications
(11 citation statements)
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“…Our benchmark detection limit is also lower than that reported by Nieradka et al . [ 82 ], (50 μg/mL), who used self assembled monolayers to discriminate between LPS from different strains of Hafnia alvei . We observed a much lower variability between the assay replicates ( S1 Fig ) as compared to the benchmark assay at 25 μg/mL LPS.…”
Section: Resultsmentioning
confidence: 99%
“…Our benchmark detection limit is also lower than that reported by Nieradka et al . [ 82 ], (50 μg/mL), who used self assembled monolayers to discriminate between LPS from different strains of Hafnia alvei . We observed a much lower variability between the assay replicates ( S1 Fig ) as compared to the benchmark assay at 25 μg/mL LPS.…”
Section: Resultsmentioning
confidence: 99%
“…QCM has been used to observe the formation of supported lipid bilayers in real-time [ 101 ] and lipid exchange between a bilayer and vesicle [ 102 ]. The latter measures mechanical bending of a receptor-functionalized microcantilever upon binding of the target molecule, as demonstrated for LPS [ 103 ]. Another example is the measurement of the interaction between amyloid-β and the cell membrane as an early indicator of Alzheimer's disease [ 104 ].…”
Section: Methods For Detecting Lipid and Amphiphilic Biomarkersmentioning
confidence: 99%
“…A colony blot-radioimmunoassay based on murine monoclonal antibodies was set up and revealed to successfully detect endotoxin from purified Haemophilus ducreyi at a level of 25 pg/mL. 72 Nieradka et al 73 developed a microcantilever array biosensor with monoclonal antibodies to detect gram-negative bacteria LPS. Luk and Lindberg 74 coupled monoclonal antibodies specific for Salmonella LPS to magnetic beads and developed a rapid immunomagnetic assay with a sensitivity of 10 3 to 10 4 bacteria/mL.…”
Section: Endotoxin Detection Methodsmentioning
confidence: 99%