Microcapillary LAMP for rapid and sensitive detection of pathogen in bovine semen

Ali, Syed Atif; Boby, Nongthombam; Preena, P.; Singh, S. D.; Kaur, Gurpreet; Ghosh, Subrata; Nandi, Sukdeb; Chaudhuri, Pallab

doi:10.1080/10495398.2020.1863225

Cited by 2 publications

(2 citation statements)

References 32 publications

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“…Cilia et al stated that the presence of serovar Bratislava in boars could be transmitted to sows during mating season (6) and a similar probability of transmission in ovines has been reported (25,26). Leptospira has been detected in semen (27)(28)(29)(30) and oviducts (7,31) of cattle. Reproductive problems have been encountered due to genital leptospirosis like early fetal death (8,32), abortion (33,34), lower fertility (34), estrus repetition (35) in mares, swine and cattle.…”

Section: Discussionmentioning

confidence: 92%

Transient Presence of Live Leptospira interrogans in Murine Testes

et al. 2022

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Section: Discussionmentioning

confidence: 92%

Transient Presence of Live Leptospira interrogans in Murine Testes

et al. 2022

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“…Various test methods have been developed for the diagnosis of BoHV-1, including loop-mediated isothermal amplification assay, duplex real-time PCR and droplet digital PCR assays ( Marin et al, 2016 ; Peltzer et al, 2021 ; Ali et al, 2022 ; Yu B. et al, 2022 ; Yu Z. et al, 2022 ). Both droplet digital PCR assays and loop-mediated isothermal amplification are very sensitive methods for the diagnosis of BoHV-1 infections; however, these methods can only be performed by personnel with technical expertise in well-equipped laboratories.…”

Section: Discussionmentioning

confidence: 99%

Development and evaluation of a time-resolved fluorescence labelled immunochromatographic strip assay for rapid and quantitative detection of bovine herpesvirus 1

Liu,

Zhang,

Cheng

et al. 2024

Front. Microbiol.

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Bovine herpes virus 1 (BoHV-1) causes a wide variety of diseases in wild and domestic cattle. The most widely used method for viral identification is real-time PCR, which can only be performed in laboratories using sophisticated instruments by expert personnel. Herein, we developed an ultrasensitive time-resolved fluorescence lateral flow immunochromatographic strip (ICS) assay for detecting BoHV-1 in bovine samples using a monoclonal antibody against BoHV-1 labelled with fluorescent microspheres, which can be applied in any setting. The intact process from sample collection to final result can be achieved in 15 min. The limit of detection of the assay for BoHV-1 was 102 TCID50/100 μL. The coincidence rate of the ICS method and real-time PCR recommended by the World Organization for Animal Health (WOAH) was 100% for negative, 92.30% for positive, and 95.42% for total, as evaluated by the detection of 131 clinical samples. This detection method was specifically targeted to BoHV-1, not exhibiting cross-reactivity with other bovine pathogens including BoHV-5. We developed an ICS assay equipped with a portable instrument that offers a sensitive and specific platform for the rapid and reliable detection of BoHV-1 in the field. The Point-of-Care test of BoHV-1 is suitable for the screening and surveillance of BoHV-1 in dairy herds.

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Microcapillary LAMP for rapid and sensitive detection of pathogen in bovine semen

Cited by 2 publications

References 32 publications

Transient Presence of Live Leptospira interrogans in Murine Testes

Transient Presence of Live Leptospira interrogans in Murine Testes

Development and evaluation of a time-resolved fluorescence labelled immunochromatographic strip assay for rapid and quantitative detection of bovine herpesvirus 1

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