Microcarrier Cell Culture Technology

Blüml, G.

doi:10.1007/978-1-59745-399-8_5

Cited by 30 publications

(33 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4,81 They increase the surface area available for cell adhesion and growth, allowing high cell density and productivity. 82,83 Microcarrier characteristics, such as size, density, and surface charge, are important for their applicability in industrial technologies. 10,11 They should be small to maximize growth surface area, nontoxic, autoclavable, suitable for microscopic monitoring of cell growth and have densities that allow them to be easily kept in suspension, as well as good adhesion properties and high batch-to-batch consistence.…”

Section: Systems For Large-scale Productionmentioning

confidence: 99%

Technological progresses in monoclonal antibody production systems

Rodrigues¹,

Costa²,

Henriques³

et al. 2009

Biotechnology Progress

101

View full text Add to dashboard Cite

Monoclonal antibodies (mAbs) have become vitally important to modern medicine and are currently one of the major biopharmaceutical products in development. However, the high clinical dose requirements of mAbs demand a greater biomanufacturing capacity, leading to the development of new technologies for their large-scale production, with mammalian cell culture dominating the scenario. Although some companies have tried to meet these demands by creating bioreactors of increased capacity, the optimization of cell culture productivity in normal bioreactors appears as a better strategy. This review describes the main technological progresses made with this intent, presenting the advantages and limitations of each production system, as well as suggestions for improvements. New and upgraded bioreactors have emerged both for adherent and suspension cell culture, with disposable reactors attracting increased interest in the last years. Furthermore, the strategies and technologies used to control culture parameters are in constant evolution, aiming at the on-line multiparameter monitoring and considering now parameters not seen as relevant for process optimization in the past. All progresses being made have as primary goal the development of highly productive and economic mAb manufacturing processes that will allow the rapid introduction of the product in the biopharmaceutical market at more accessible prices.

show abstract

Section: Systems For Large-scale Productionmentioning

confidence: 99%

Technological progresses in monoclonal antibody production systems

Rodrigues¹,

Costa²,

Henriques³

et al. 2009

Biotechnology Progress

101

View full text Add to dashboard Cite

show abstract

“…Animal cell cultures are more and more realized to produce various complex biopharmaceuticals (Blüml 2007;Decker et al 2007). There are two kinds of animal cell cultures: suspended cell cultures and adhering cell cultures.…”

Section: Introductionmentioning

confidence: 99%

Segregated Model of Adherent Cell Culture in a Fixed-Bed Bioreactor

Gelbgras¹,

Wylock²,

Drugmand³

et al. 2011

Chemical Product and Process Modeling

View full text Add to dashboard Cite

In this work, a mathematical model of a fixed-bed bioreactor for the animal cell culture is developed to study the optimization and the scale-up of this bioreactor. Several cell populations are considered: the cells in suspension in the medium at the beginning of the process and the adhering cells to the fixed-bed. The model includes a capture rate kinetic of the cells in suspension by the fixed-bed and a spatial distribution of the nutrient and by-product concentrations in the fixed-bed. Therefore, the model reports the potential gradients of the cell concentrations in the fixed-bed. Some model parameters are experimentally identified and the model is validated using experimental data obtained with two pilot bioreactors. The model is used as a simulation tool to study the influence of the bioreactor design or the velocity field of the culture medium on the cell concentration gradients in the fixed-bed bioreactor and to optimize the operating conditions, the design, and the scale-up of this bioreactor.

show abstract

“…Animal cell cultures in a bioreactor are particularly relevant to produce monoclonal antibodies, viral particles … (Blüml 2007;Decker et al 2007). In this work, the VERvet Origin (VERO) cell cultures in serum free medium are cultivated in a fixed-bed bioreactor of the biotechnological company Artelis S.A..…”

Section: Introductionmentioning

confidence: 99%

Structured Model of VERO Cells Metabolism in a Fixed-Bed Bioreactor

Gelbgras¹,

Wylock²,

Drugmand³

et al. 2011

Chemical Product and Process Modeling

View full text Add to dashboard Cite

In this work, a structured mathematical model of the VERvet Origin (VERO) cell metabolism in a fixed-bed bioreactor is developed. Mass balance equations for the extra-and intracellular species are written considering the main pathways of the animal cell metabolism. From the model equations, an undetermined set of equations relating the consumption or production velocities of the extracellular species and the velocities of the metabolic reactions can be developed. The monitoring of a reference cell culture enables to transform this undetermined set of equations in a determined one. The resolution of this determined set of equations enables to analyze and characterize the cell metabolism. From the model and the cell metabolism characterization, a monitoring tool is developed to simulate the time evolution of the species concentrations when the time evolution of the cell concentration is known. This simulation tool is used to simulate five experimental cell cultures. The comparison of the experimentally determined and computed time evolutions of the species concentrations enables to validate the developed monitoring tool. The validated monitoring tool can be used to optimize the operating conditions of the culture, as the medium feeding rate, the range of the species concentrations.

show abstract

Microcarrier Cell Culture Technology

Cited by 30 publications

References 14 publications

Technological progresses in monoclonal antibody production systems

Technological progresses in monoclonal antibody production systems

Segregated Model of Adherent Cell Culture in a Fixed-Bed Bioreactor

Structured Model of VERO Cells Metabolism in a Fixed-Bed Bioreactor

Contact Info

Product

Resources

About