Microcarriers with Controllable Size via Electrified Liquid Jets and Phase Separation Technique Promote Cell Proliferation and Osteogenic Differentiation

Zhang, Jiao; Fu, Chen; Li, Linlong; Wang, Zongliang; Wang, Yu; Shi, Xincui; Zhang, Peibiao

doi:10.1021/acsabm.9b00746

Cited by 6 publications

(11 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present study, the surface of the composite microspheres became rough due to the incorporation of nBM. In Jiao's report, the surface of composite microcarriers with nHA was rougher than that of PLGA observed by ESEM 27 . This was consistent with our study.…”

Section: Resultssupporting

confidence: 92%

“…The particle sizes of the microspheres were not strictly uniform, but were normally distributed. Therefore, the settling volume of microspheres was used for the quantification of microspheres in vitro and vivo studies as previously reported 22,27,28 . First, the microspheres were immersed in PBS.…”

Section: Methodsmentioning

confidence: 99%

“…The biomimetic microspheres were prepared by electrified liquid jets and phase separation technique as our previous study. 27 First, nBM was suspended in N-methyl pyrrolidone (NMP) and added in a PLGA/NMP (7%, w/v) solution and then followed by adding Ica. The mass ratio of PLGA: nBM was 9:1 (w/w).…”

Section: Preparation Of Biomimetic Microspheresmentioning

confidence: 99%

“…Therefore, the settling volume of microspheres was used for the quantification of microspheres in vitro and vivo studies as previously reported. 22,27,28 First, the microspheres were immersed in PBS. After settling in PBS, the microspheres were taken with a pipettor and transferred into the culture plate or the defect of the animal model.…”

Section: Biocompatibility Of Biomimetic Microspheresmentioning

confidence: 99%

See 3 more Smart Citations

A novel icariin‐encapsulated PLGA/nBM composite biomimetic microspheres for bone repair

Wu,

Niu,

Xiong

et al. 2024

J of Applied Polymer Sci

Self Cite

View full text Add to dashboard Cite

Deer bone in traditional Chinese medicine is valued for its unique biomimetic bone matrix components. Icariin (Ica), which is claimed by Chinese medicine as a bone‐repairing agent, is also widely accepted. In this report, a novel Ica‐encapsulated PLGA/nano deer bone meal (Ica@PLGA/nBM) biomimetic microsphere was fabricated. The Ica could be released from microspheres in a controlled manner. In vitro results showed that the microsphere had good biocompatibility due to the addition of nBM and the MC3T3‐E1 cells could adhere to and grow well on the microspheres. Moreover, Ica and nBM in microspheres could synergically promote the ALP activity of MC3T3‐E1 cells significantly (p < 0.05), which indicated the acceleration of osteogenic differentiation. The RT‐PCR results showed that the expression levels of bone matrix proteins (Col1 & OPN) were enhanced by the composite (p < 0.05). In addition, Ica and nBM in microspheres also obviously promoted the expression of RUNX2 and BMP‐2, respectively (p < 0.05). The in vivo results demonstrated the effective promotion of Ica@PLGA/nBM on complete bony connection to cover the defect site and the bone defect repair. And the Ica(M)@PLGA/nBM microspheres provided the best repair results. All the results confirmed that the Ica@PLGA/nBM composite biomimetic microsphere has the potential for clinical application.

show abstract

Section: Resultssupporting

confidence: 92%

Section: Methodsmentioning

confidence: 99%

Section: Preparation Of Biomimetic Microspheresmentioning

confidence: 99%

Section: Biocompatibility Of Biomimetic Microspheresmentioning

confidence: 99%

See 2 more Smart Citations

A novel icariin‐encapsulated PLGA/nBM composite biomimetic microspheres for bone repair

Wu,

Niu,

Xiong

et al. 2024

J of Applied Polymer Sci

Self Cite

View full text Add to dashboard Cite

show abstract

“…The phase separation of LLPS microcarriers in an aqueous solution, results in the production of discrete liquid phases, with differing compositions. This enables the encapsulation of various bioactive compounds, such as proteins and growth factors, in various phases of the microcarrier [ 15 , 16 ]. Cell harvesting from substrates is challenging in conventional microcarrier systems, because it requires enzymatic treatment, which is frequently paired with agitation.…”

Section: Introductionmentioning

confidence: 99%

Emerging Trends in Biodegradable Microcarriers for Therapeutic Applications

2023

View full text Add to dashboard Cite

Microcarriers (MCs) are adaptable therapeutic instruments that may be adjusted to specific therapeutic uses, making them an appealing alternative for regenerative medicine and drug delivery. MCs can be employed to expand therapeutic cells. MCs can be used as scaffolds for tissue engineering, as well as providing a 3D milieu that replicates the original extracellular matrix, facilitating cell proliferation and differentiation. Drugs, peptides, and other therapeutic compounds can be carried by MCs. The surface of the MCs can be altered, to improve medication loading and release, and to target specific tissues or cells. Allogeneic cell therapies in clinical trials require enormous volumes of stem cells, to assure adequate coverage for several recruitment locations, eliminate batch to batch variability, and reduce production costs. Commercially available microcarriers necessitate additional harvesting steps to extract cells and dissociation reagents, which reduces cell yield and quality. To circumvent such production challenges, biodegradable microcarriers have been developed. In this review, we have compiled key information relating to biodegradable MC platforms, for generating clinical-grade cells, that permit cell delivery at the target site without compromising quality or cell yields. Biodegradable MCs could also be employed as injectable scaffolds for defect filling, supplying biochemical signals for tissue repair and regeneration. Bioinks, coupled with biodegradable microcarriers with controlled rheological properties, might improve bioactive profiles, while also providing mechanical stability to 3D bioprinted tissue structures. Biodegradable materials used for microcarriers have the ability to solve in vitro disease modeling, and are advantageous to the biopharmaceutical drug industries, because they widen the spectrum of controllable biodegradation and may be employed in a variety of applications.

show abstract

Biodegradable GdPO4·H2O/PLGA microcarriers for stem cell delivery and non-invasive MRI translocation tracing

Zhu

Wang

et al. 2022

J Mater Sci

View full text Add to dashboard Cite

Microcarriers with Controllable Size via Electrified Liquid Jets and Phase Separation Technique Promote Cell Proliferation and Osteogenic Differentiation

Cited by 6 publications

References 28 publications

A novel icariin‐encapsulated PLGA/nBM composite biomimetic microspheres for bone repair

A novel icariin‐encapsulated PLGA/nBM composite biomimetic microspheres for bone repair

Emerging Trends in Biodegradable Microcarriers for Therapeutic Applications

Biodegradable GdPO4·H2O/PLGA microcarriers for stem cell delivery and non-invasive MRI translocation tracing

Contact Info

Product

Resources

About