Microchip electrophoresis: A suitable analytical technique for pharmaceuticals quality control? A critical review

Dispas, Amandine; Emonts, Paul; Fillet, Marianne

doi:10.1016/j.trac.2021.116266

Cited by 11 publications

(9 citation statements)

References 43 publications

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“…Additionally, all separation modes available in CE could be used in the ME, allowing for a broad range of applications [ 19 ]. ME allows for integrating multiple functions such as injection, separation, and detection on a single microchip with typical channel lengths of 1–10 cm [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Rapid determination of NSAIDs by capillary and microchip electrophoresis with capacitively coupled contactless conductivity detection in wastewater

et al. 2022

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A simple, rapid method using CE and microchip electrophoresis with C4D has been developed for the separation of four nonsteroidal anti‐inflammatory drugs (NSAIDs) in the environmental sample. The investigated compounds were ibuprofen (IB), ketoprofen (KET), acetylsalicylic acid (ASA), and diclofenac sodium (DIC). In the present study, we applied for the first time microchip electrophoresis with C4D detection to the separation and detection of ASA, IB, DIC, and KET in the wastewater matrix. Under optimum conditions, the four NSAIDs compounds could be well separated in less than 1 min in a BGE composed of 20 mM His/15 mM Tris, pH 8.6, 2 mM hydroxypropyl‐beta‐cyclodextrin, and 10% methanol (v/v) at a separation voltage of 1000–1200 V. The proposed method showed excellent repeatability, good sensitivity (LODs ranging between 0.156 and 0.6 mg/L), low cost, high sample throughputs, portable instrumentation for mobile deployment, and extremely lower reagent and sample consumption. The developed method was applied to the analysis of pharmaceuticals in wastewater samples with satisfactory recoveries ranging from 62.5% to 118%.

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Section: Introductionmentioning

confidence: 99%

Rapid determination of NSAIDs by capillary and microchip electrophoresis with capacitively coupled contactless conductivity detection in wastewater

et al. 2022

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“…114−117 Recently, microchip electrophoresis (MCE) has emerged among the various analytical strategies based on electrophoresis; however, several challenging aspects of this device still need to be addressed. 118 For example, the analytes lead to nonspecific adsorptions because the hydrophobic nature of PDMS hampers the separation efficiency of the PDMS-based MCE. Besides, because of the hydrophobicity of PDMS-based microchannels, bubbles tend to form when an aqueous buffer solution is introduced.…”

Section: Biomedical Applications Of Phospholipidmentioning

confidence: 99%

“…MPC polymers are also utilized for coatings of microchips engineered for the purpose of protein electrophoresis. Electrophoresis is an electrokinetic process extensively used in the separation and detection of proteins, DNA, and RNA. − Recently, microchip electrophoresis (MCE) has emerged among the various analytical strategies based on electrophoresis; however, several challenging aspects of this device still need to be addressed . For example, the analytes lead to nonspecific adsorptions because the hydrophobic nature of PDMS hampers the separation efficiency of the PDMS-based MCE.…”

Section: Biomedical Applications Of Phospholipid Nanobiomaterialsmentioning

confidence: 99%

Emerging Phospholipid Nanobiomaterials for Biomedical Applications to Lab-on-a-Chip, Drug Delivery, and Cellular Engineering

Rahimnejad

Rabiee

Ahmadi

et al. 2021

ACS Appl. Bio Mater.

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The design of advanced nanobiomaterials to improve analytical accuracy and therapeutic efficacy has become an important prerequisite for the development of innovative nanomedicines. Recently, phospholipid nanobiomaterials including 2-methacryloyloxyethyl phosphorylcholine (MPC) have attracted great attention with remarkable characteristics such as resistance to nonspecific protein adsorption and cell adhesion for various biomedical applications. Despite many recent reports, there is a lack of comprehensive review on the phospholipid nanobiomaterials from synthesis to diagnostic and therapeutic applications. Here, we review the synthesis and characterization of phospholipid nanobiomaterials focusing on MPC polymers and highlight their attractive potentials for applications in micro/nanofabricated fluidic devices, biosensors, lab-on-a-chip, drug delivery systems (DDSs), COVID-19 potential usages for early diagnosis and even treatment, and artificial extracellular matrix scaffolds for cellular engineering.

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“…Despite the advantages of stable EOF in glass microchips, their processing cost is high, and the tiny bare glass channels are highly susceptible to contamination in complex sample analysis. In plastic microchips, such as cyclic olefin copolymer (COC) microchip, stable EOF can be obtained by dynamically or statically modifying the channel [24]. Static coating through surface modification is a common approach to obtain stable EOF in plastic microchip [25,26].…”

Section: Introductionmentioning

confidence: 99%

An integrated plastic microchip for enhancing electrophoretic separation using tunable pressure‐driven backflows

et al. 2022

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Microfluidic CE (MCE) is an effective solution for rapid and sensitive determination of multiple analytes. Herein, a dynamic coated cyclic olefin copolymer microchip was developed having an on‐chip micropump for fluid velocity adjusting in electrophoretic separations. This micropump was fabricated by constructing a polyacrylamide gel membrane at one channel terminal. Once applying electric field across the membrane, a pressure‐driven flow generated automatically to balance the electroosmotic flow (EOF) mismatch at the channel‐membrane interface. The influence of gel precursor concentration and operating voltages on the fluid velocity was carefully evaluated. Moreover, the highly integration of injection, separation, and pumping units of the MCE system minimized the dead volume and provides satisfied column efficiency. Experiments showed that by adjusting of pumping voltage reduced the fluid velocity by a factor of 6, resulting six‐ and threefold resolving power enhancements of rhodamine dye mixture and amino acid mixture, respectively. Furthermore, the developed MCE method was applied for rhodamines and amino acids quantitation in food and cosmetics, with standard addition recoveries of 87.3–106.9% and 89.9–117.4%, respectively. These results were also confirmed by standard HPLC method, revealing the application potential in fast and onsite analysis of complex samples.

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Microchip electrophoresis: A suitable analytical technique for pharmaceuticals quality control? A critical review

Cited by 11 publications

References 43 publications

Rapid determination of NSAIDs by capillary and microchip electrophoresis with capacitively coupled contactless conductivity detection in wastewater

Rapid determination of NSAIDs by capillary and microchip electrophoresis with capacitively coupled contactless conductivity detection in wastewater

Emerging Phospholipid Nanobiomaterials for Biomedical Applications to Lab-on-a-Chip, Drug Delivery, and Cellular Engineering

An integrated plastic microchip for enhancing electrophoretic separation using tunable pressure‐driven backflows

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