Microchip Electrophoresis Using Linear-Imaging UV Detector

Abstract

We have studied on microchip-cyclodextrin electrokinetic chromatography (MC-CDEKC) analysis of enantiomers and microchip-isoelectric focusing (MC-IEF) of proteins by using a commercially available microchip electrophoresis (MCE) instrument equipped with a linearimaging UV detector, which allows real-time measurements of concentration profiles in a microchannel. In the MC-CDEKC analysis of racemic 1-aminoindane, sulfated-β-cyclodextrin (S-β-CD) was used as a pseudo-stationary phase. Effect of the concentration of S-β-CD on the separation process was investigated. In the MC-IEF analysis of proteins, on the other hand, we studied the processes of a pH gradient formation and protein focusing. Furthermore, effect of the channel length and applied electric field strength on the separation performance of proteins in MC-IEF was studied.