Microcrustaceos y Vibrio cholerae O1 viable no cultivable (VNC): resultados en la Cuenca del Rio Sali, Tucuman, Argentina

Mitrovich, Cecilia Locascio de; Gamundi, Alcira Villagra de; Silva, Clara; Cecilia, Marta; Binsztein, Norma

doi:10.3856/vol38-issue1-fulltext-7

Cited by 2 publications

(1 citation statement)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2K and 2L did not seem pathogenic, and not even active in the digestive tract, because they were not detected using culture methods. They transited likely in a viable, but noncultivable state similar to that associated with zooplankton (Thomas et al, 2006;Locascio de Mitrovich et al, 2010). To our The indices of dominance, Shannon's entropy, Menhinick's richness, Margalef's richness and Fisher's a were found to be significantly different, depending on the diet by two-way ANOVA, while there was no influence of the sampling segment or significant interaction.…”

Section: Discussionmentioning

confidence: 52%

Influence of the diet on the microbial diversity of faecal and gastrointestinal contents in gilthead sea bream (Sparus aurata) and intestinal contents in goldfish (Carassius auratus)

Silva

Nicoli

Zambonino-Infante

et al. 2011

FEMS Microbiology Ecology

106

View full text Add to dashboard Cite

Fish intestinal microbiota changes with the diet and this effect is of particular interest considering the increasing substitution of fish meal by plant protein sources. The objective of this work was to study the effects of partial substitution of fish meal with lupin and rapeseed meals on gut microbiota of the gilthead sea bream (Sparus aurata) and in goldfish (Carassius auratus). Faecal, gastrointestinal and intestinal contents were characterized using culture-based and molecular methods. Vibrionaceae was high in faeces and in the intestine of sea bream, while a more diverse microbiota was retrieved from the stomach, where Bacillales and Flavobacteriaceae appeared to be influenced by the diet. PCR-denaturing gradient gel electrophoresis profiles revealed a high diversity of the microbiota transiting in the sea bream digestive tract, with a shift between gastric and intestinal communities, especially in the group fed with lupin meal. The goldfish was different, with a predominance of Aeromonas spp., Shewanella putrefaciens and Staphylococcus spp. among the aerotolerant-cultivable bacteria. The culture-independent methods revealed the presence of anaerobes like Cetobacterium somerae, and that of Vibrio spp., likely in a viable, but noncultivable state. There was a trend towards decreasing diversity in goldfish microbiota with the partial substitution by lupin, which seemed to inhibit some taxa.

show abstract

Section: Discussionmentioning

confidence: 52%