MicroCT optimisation for imaging fascicular anatomy in peripheral nerves

Thompson, Nicole; Ravagli, Enrico; Mastitskaya, Svetlana; Iacoviello, Francesco; Aristovich, Kirill; Perkins, Justin; Shearing, Paul R.; Holder, David

doi:10.1016/j.jneumeth.2020.108652

Cited by 37 publications

(23 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, one study reported a protocol for staining rat sciatic and pig vagus nerves, optimization of computational methods for high-resolution three-dimensional images of nerve fascicles, and development of image analysis techniques to facilitate segmentation and tracing of the fascicles. 40 The fascicle morphology measurements obtained from our microCT data were similar to those obtained by other groups. 37 Here, we demonstrated the unique value of microCT to quantify fascicular splitting and merging of the human cVN.…”

Section: Mainsupporting

confidence: 87%

Fascicles Split or Merge Every ~560 Microns Within the Human Cervical Vagus Nerve

Upadhye

Kolluru

Druschel

et al. 2021

Preprint

View full text Add to dashboard Cite

1AbstractVagus nerve stimulation (VNS) is FDA approved for stroke rehabilitation, epilepsy and depression; however, the underlying vagus functional anatomy underlying the implant is poorly understood. We used microCT to quantify fascicular structure and neuroanatomy within human cervical vagus nerves. Fascicles split or merged every ~560 μm (17.8 ± 6.1 events/cm). The high degree of splitting and merging of fascicles in humans may explain the clinical heterogeneity in patient responses.

show abstract

Section: Mainsupporting

confidence: 87%

Fascicles Split or Merge Every ~560 Microns Within the Human Cervical Vagus Nerve

Upadhye

Kolluru

Druschel

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Achieving a better understanding of VN anatomy is the most important step toward improving sVNS techniques. While great progress has been made in this area (Thompson et al, 2019(Thompson et al, , 2020Settell et al, 2020), it is still not established definitively whether fibers in the VN are arranged by fiber-type or by innervated organ, with current evidence inconclusive. If VN fibers are arranged both by fiber-type and somatotopically, then it would be necessary to develop hybrid sVNS techniques that are both fiber-selective and spatially selective.…”

Section: Discussionmentioning

confidence: 99%

“…There are two VNs (left and right), but convention is to refer to the VN in the singular, even though there are some functional differences between the two VNs; most importantly, the right VN innervates the sinoatrial node of the heart whereas the left VN innervates the atrioventricular node. Despite extensive research over the last century, the functional fascicular anatomy of this complex nerve remains poorly understood ( Thompson et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Selective Neuromodulation of the Vagus Nerve

2021

Self Cite

View full text Add to dashboard Cite

Vagus nerve stimulation (VNS) is an effective technique for the treatment of refractory epilepsy and shows potential for the treatment of a range of other serious conditions. However, until now stimulation has generally been supramaximal and non-selective, resulting in a range of side effects. Selective VNS (sVNS) aims to mitigate this by targeting specific fiber types within the nerve to produce functionally specific effects. In recent years, several key paradigms of sVNS have been developed—spatially selective, fiber-selective, anodal block, neural titration, and kilohertz electrical stimulation block—as well as various stimulation pulse parameters and electrode array geometries. sVNS can significantly reduce the severity of side effects, and in some cases increase efficacy of the treatment. While most studies have focused on fiber-selective sVNS, spatially selective sVNS has demonstrated comparable mitigation of side-effects. It has the potential to achieve greater specificity and provide crucial information about vagal nerve physiology. Anodal block achieves strong side-effect mitigation too, but is much less specific than fiber- and spatially selective paradigms. The major hurdle to achieving better selectivity of VNS is a limited knowledge of functional anatomical organization of vagus nerve. It is also crucial to optimize electrode array geometry and pulse shape, as well as expand the applications of sVNS beyond the current focus on cardiovascular disease.

show abstract

“…Reference images of nerve transversal sections with fascicles clearly visible were collected by MicroCT imaging to obtain fascicle ground truth locations in the form of CoM coordinates ( Thompson et al, 2020 ; Ravagli et al, 2019 , 2020 ).…”

Section: Methodsmentioning

confidence: 99%

Fascicle localisation within peripheral nerves through evoked activity recordings: A comparison between electrical impedance tomography and multi-electrode arrays

Ravagli

Mastitskaya

Thompson

et al. 2021

Journal of Neuroscience Methods

Self Cite

View full text Add to dashboard Cite

Highlights Fascicle localization power of EIT and MEA was evaluated against MicroCT reference scans. Tested MEAs based on silicon shanks and carbon fibers in rat sciatic nerves. Fast neural EIT and multi-electrode arrays can detect evoked activity within the nerve. EIT and carbon fiber MEA have better source localization power than silicon MEA. Technical recommendations for insertion of MEAs into the nerves are provided.

show abstract

MicroCT optimisation for imaging fascicular anatomy in peripheral nerves

Cited by 37 publications

References 54 publications

Fascicles Split or Merge Every ~560 Microns Within the Human Cervical Vagus Nerve

Fascicles Split or Merge Every ~560 Microns Within the Human Cervical Vagus Nerve

Selective Neuromodulation of the Vagus Nerve

Fascicle localisation within peripheral nerves through evoked activity recordings: A comparison between electrical impedance tomography and multi-electrode arrays

Contact Info

Product

Resources

About