Microcystin diversity in a Planktothrix rubescens population from Lake Albano (Central Italy)

Messineo, Valentina; Mattei, Daniela; Melchiorre, Serena; Salvatore, Giovanna; Bogialli, Sara; Salzano, R.; Mazza, Roberto; Capelli, Gioia; Bruno, Marco

doi:10.1016/j.toxicon.2006.04.006

Cited by 52 publications

(40 citation statements)

References 48 publications

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“…These results allowed to associate the potential producing species with the different MC variants and furthermore to obtain a model which explained more than 80% of the variation in MC concentration in this system. The association between P. rubescens and MC-dem-RR and -dem-LR was also confirmed by the 10 monoclonal isolated strains producing only the two variants, and is in line with data from other Italian lakes (Messineo et al, 2006;Manganelli et al, 2010Manganelli et al, , 2016.…”

Section: Discussionsupporting

confidence: 88%

Cyanobacterial dynamics and toxins concentrations in Lake Alto Flumendosa, Sardinia, Italy

et al. 2017

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Seasonal blooms of cyanobacteria (CB) are a typical feature of Lake Alto Flumendosa (Sardinia, Italy). The waters of this lake are used for drinking water supply, for agricultural and industrial uses, and fish farming activities. Since cyanotoxins are not monitored in edible organisms, diet could be a relevant route of human exposure. CB also represent a threat for the health of wild and domestic animals that use lake water for beverage. Therefore, to characterize the CB community and assess the risk for human and animal population, CB dynamic, mcyB+ fraction, and microcystins (MCs) concentration have been followed monthly for 18 months, in three stations. Results confirmed the presence of several toxigenic species. Planktothrix rubescens dominated between August 2011 and April 2012 (3.5×106 cells L-1), alternating with Woronichinia naegeliana (8×106 cells L-1) and Microcystis botrys (9×105 cells L-1). Dolichospermum planctonicum was always present at low densities (104 cells L-1). MCs were detected, at values well below the 1 µg L-1 threshold of WHO for drinking water. The molecular analysis of mcyB gene for P. rubescens indicated the presence of a persistent toxic population (average 0.45 mcyB/16S rDNA). Highly significant linear regressions were found between P. rubescens and the sum of the demethylated MC variants, and between M. botrys and the sum of MC-LR and MC-LA, also when co-occurring, suggesting that these two species were responsible for different MC patterns production. The regression lines indicated a quite stable MC cell quota. However, in some spotted samples very different values were obtained for both MC concentrations and cell quota (from 10-fold lower to 30-40-fold higher than the ‘average’) showing an unexpected significant variability in the rate of toxin production. The relatively low cell densities during the monitoring period is consistent with the low-to absent MC contamination level found in trout muscle; however, the analytical method was affected by low recovery, probably due to MC-protein binding. Our results show that, during the study period, no risk of exposure for the human and animal population occurred. However, the persistence of a complex CB community characterised by a significant toxic fraction suggests the need for periodic monitoring activity. Particularly, the hidden deep summer P. rubescens blooms, located where water is taken for drinking water supply, and M. botrys, able to produce the most toxic MC variants with high cell quota, should be kept under control. The documentation and interpretation of sudden changes in toxins concentrations deserve special attention. This is particularly relevant in proximity of fish farming plants and water catchment sites.

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Section: Discussionsupporting

confidence: 88%

Cyanobacterial dynamics and toxins concentrations in Lake Alto Flumendosa, Sardinia, Italy

et al. 2017

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“…Planktothrix rubescens (De Candolle ex Gomont) Anagnostidis and Koma´rek is the most typical and abundant cyanobacterium in many deep Italian lakes (Messineo et al, 2006;D'Alelio et al, 2011;Salmaso et al, 2012aSalmaso et al, , 2012b and in many Italian reservoirs (Naselli-Flores et al, 2007). In this paper, we report the results of a study carried out in two populations of P. rubescens in two nearby subalpine lakes in Northeastern Italy, namely lakes Garda (a natural large and deep lake) and Ledro (a natural lake with artificial water regulation for hydroelectric purpose).…”

Section: Introductionmentioning

confidence: 99%

Strict coupling between the development ofPlanktothrix rubescensand microcystin content in two nearby lakes south of the Alps (lakes Garda and Ledro)

Salmaso

Adriano

Shams

et al. 2013

Ann. Limnol. - Int. J. Lim.

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-Cyanobacteria and their principal cyanotoxins were studied in the largest Italian lake (Lake Garda, 65 m a.s.l.) and in a near smaller reservoir (Lake Ledro, 652 m a.s.l.). The two lakes share a fraction of water due to a pipe pumping water from and into the lakes and the same dominant cyanobacterial species (Planktothrix rubescens). Despite the differences in the concentrations of cyanotoxins (mostly microcystins, MCs) and biovolumes of P. rubescens (BV Pr ) (over one order of magnitude), the Bayesian analyses linking these two variables showed striking similarities, suggesting the existence of similar toxic genotypes colonizing the two water bodies and a constitutive MC production. It was stressed that a greater sensitivity and reliability in the management strategies aimed at minimizing the risks due to cyanobacteria should also contemplate the use of specific lake-tailored models linking MCs and BV Pr .

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“…For a more accurate risk assessment a complete molecular profiling of the toxic compounds is required, but unfortunately this approach is laborious and costly. Several papers have been published about the occurrence of toxic cyanobacteria in Italian fresh water (Aktan et al 2009, Barone et al 2010, Bruno et al 1994, Manganelli et al 2010, Messineo et al 2006, Sechi and Lugliè 1992, Viaggiu et al 2004. These authors have shown that potentially toxic blooms can occur not only in eutrophic water basins but also in oligotrophic ones.…”

Section: Introductionmentioning

confidence: 99%

Diversity and distribution of cyanobacterial toxins in the Italian subalpine lacustrine district

Cerasino

Salmaso

2012

Oceanological and Hydrobiological Studies

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Nine lakes (Garda, Maggiore, Como, Iseo, Lugano, Idro, Pusiano, Ledro, and Levico) located in the Italian subalpine lacustrine district were chosen for a comparative study of the diversity and distribution of cyanobacterial toxins. All nine lakes are known to host different toxic cyanobacteria, mainly Planktothrix rubescens. An analytical protocol has been used, based on LC-MS technique, for determining 20 different toxins (14 microcystins, 2 nodularins, anatoxin-a and 3 cylindrospermopsins). In the investigation, microcystins were found in all lakes on all sampling dates. Anatoxin-a could be detected in 6 lakes, but it was only present in 4 lakes on all sampling dates (lakes Garda, Iseo, Como, and Maggiore). Nodularins and cylindrospermopsins were not detected at all. The number of microcystins detected in the survey was 9, but 4 were the most abundant (dmRR, YR, dmLR, and LR); they were differently distributed among the lakes, thus providing each lake a unique toxic potential. Statistical analysis showed positive * Corresponding Author e-mail: leonardo.cerasino@iasma.it correlations between total microcystin concentrations and eutrophication indicators and also between anatoxin-a concentrations and water temperature, suggesting that anthropic and climate-related stressors can have different impacts on the presence of different cyanotoxins.

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Microcystin diversity in a Planktothrix rubescens population from Lake Albano (Central Italy)

Cited by 52 publications

References 48 publications

Cyanobacterial dynamics and toxins concentrations in Lake Alto Flumendosa, Sardinia, Italy

Cyanobacterial dynamics and toxins concentrations in Lake Alto Flumendosa, Sardinia, Italy

Strict coupling between the development ofPlanktothrix rubescensand microcystin content in two nearby lakes south of the Alps (lakes Garda and Ledro)

Diversity and distribution of cyanobacterial toxins in the Italian subalpine lacustrine district

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