Microcystin interferes with defense against high oxidative stress in harmful cyanobacteria

Schuurmans, J. Merijn; Brinkmann, Bregje W.; Makower, Alexander; Dittmann, Elke; Huisman, Jef; Matthijs, H.C.P.

doi:10.1016/j.hal.2018.07.008

Cited by 61 publications

(63 citation statements)

References 67 publications

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“…Previous studies demonstrated that H 2 O 2 reduced the content of MCs in Microcystis aeruginosa by down‐regulating the expression of the relevant genes mcy H, mcy A, and mcy D and directly decomposing the MCs via oxidation (Schuurmans et al. ). However, glyphosate was found to stimulate the synthesis of MCs (Wu et al.…”

Section: Discussionmentioning

confidence: 99%

Influence of mixed antibiotics on Microcystis aeruginosa during the application of glyphosate and hydrogen peroxide algaecides

Liu

Zhang

et al. 2019

Journal of Phycology

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Antibiotics regulate various physiological functions in cyanobacteria and may interfere with the control of cyanobacterial blooms during the application of algaecides. In this study, Microcystis aeruginosa was exposed to H2O2 and glyphosate for 7 d in the presence of coexisting mixed antibiotics (amoxicillin, spiramycin, tetracycline, ciprofloxacin, and sulfamethoxazole) at an environmentally relevant concentration of 100 ng · L−1. The mixed antibiotics significantly (P < 0.05) alleviated the growth inhibition effect of 15–45 μM H2O2 and 40–60 mg · L−1 glyphosate. According to the increased contents of chlorophyll a and protein, decreased content of malondialdehyde, and decreased activities of superoxide dismutase and glutathione S‐transferase, antibiotics may reduce the toxicity of the two algaecides through the stimulation of photosynthesis and the reduction in oxidative stress. The presence of coexisting antibiotics stimulated the production and release of microcystins in the M. aeruginosa exposed to low concentrations of algaecides and posed an increased threat to aquatic environments. To eliminate the secondary pollution caused by microcystins, high algaecide doses that are ≥45 μM for H2O2 and ≥60 mg · L−1 for glyphosate are recommended. This study provides insights into the ecological hazards of antibiotic contaminants and the best management practices for cyanobacterial removal under combined antibiotic pollution conditions.

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Section: Discussionmentioning

confidence: 99%

Influence of mixed antibiotics on Microcystis aeruginosa during the application of glyphosate and hydrogen peroxide algaecides

Liu

Zhang

et al. 2019

Journal of Phycology

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“…Some external or internal stimulation may cause both an increased content of intracellular MC per cell and up-regulation of mcyE gene expression [13]. On the other hand, Schuurmans et al [3] hypothesised that MC may suppress the activity of proteins involved in H 2 O 2 degradation. Furthermore, some opposite conclusions have been presented regarding the impact of H 2 O 2 on MC production by toxic strains.…”

Section: The Impact Of H 2 O 2 On the MC Production By M Aeruginosamentioning

confidence: 99%

“…Furthermore, some opposite conclusions have been presented regarding the impact of H 2 O 2 on MC production by toxic strains. Low, environmentally relevant concentrations of H 2 O 2 may induce MC production that is necessary for the protection against such mild oxidative stress [1], however higher H 2 O 2 doses result in a decrease in the total (extra-and intra-cellular) MC content [3]. As suggested previously [10], 2-5 mg L −1 of H 2 O 2 is probably too little to induce cyanobacterial lysis in dense blooms and can stimulate the accumulation of cell-bound (intracellular) MC as a reaction to oxidative stress.…”

Section: The Impact Of H 2 O 2 On the MC Production By M Aeruginosamentioning

confidence: 99%

“…According to these data, it is still an open question what H 2 O 2 doses (typically between 2 and 10 mg L −1 ) should be recommended to eliminate toxic cyanobacteria (and ideally cyanotoxins) but at the same time to cause a minimal impact on other biota. Schuurmans et al [3] suggested that the dose should depend on the initial cell density in order to achieve a necessary level per cell.…”

Section: The Impact Of H 2 O 2 On the MC Production By M Aeruginosamentioning

confidence: 99%

“…Thus, fast reduction of MC concentration by H 2 O 2 in blooming water bodies has been questioned because the physiological response may include enhanced MC production. On the other hand, recent research on MC involvement in defence against oxidative stress [3] documented that MC-producing strains are less resistant to H 2 O 2 than non-toxic strains. Further, field experiments have provided other arguments that H 2 O 2 used alone is not effective in fast MC elimination [4,5].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Effect of a Combined Hydrogen Peroxide-MlrA Treatment on the Phytoplankton Community and Microcystin Concentrations in a Mesocosm Experiment in Lake Ludoš

Dziga

Tokodi

Drobac

et al. 2019

Toxins

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Harmful cyanobacteria and their toxic metabolites constitute a big challenge for the production of safe drinking water. Microcystins (MC), chemically stable hepatotoxic heptapeptides, have often been involved in cyanobacterial poisoning incidents. A desirable solution for cyanobacterial management in lakes and ponds would eliminate both excess cyanobacteria and the MC that they potentially produce and release upon lysis. Hydrogen peroxide (H 2 O 2 ) has recently been advocated as an efficient means of lysing cyanobacteria in lakes and ponds, however H 2 O 2 (at least when used at typical concentrations) cannot degrade MC in environmental waters. Therefore, mesocosm experiments combining the cyanobacteria-lysing effect of H 2 O 2 and the MC-degrading capacity of the enzyme MlrA were set up in the highly eutrophic Lake Ludoš (Serbia). The H 2 O 2 treatment decreased the abundance of the dominant cyanobacterial taxa Limnothrix sp., Aphanizomenon flos-aquae, and Planktothrix agardhii. The intracellular concentration of MC was reduced/eliminated by H 2 O 2 , yet the reduction of the extracellular MC could only be accomplished by supplementation with MlrA. However, as H 2 O 2 was found to induce the expression of mcyB and mcyE genes, which are involved in MC biosynthesis, the use of H 2 O 2 as a safe cyanobacteriocide still requires further investigation. In conclusion, the experiments showed that the combined use of H 2 O 2 and MlrA is promising in the elimination of both excess cyanobacteria and their MC in environmental waters. Key Contribution:A new concept has been proposed for rapid and efficient mitigation of MC-producing cyanobacterial biomass through a combined treatment with H 2 O 2 and microcystinase (MlrA).

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Effects of H2O2 on growth, metabolic activity and membrane integrity in three strains of Microcystis aeruginosa

Foo

Chapman

Hartnell

et al. 2020

Environ Sci Pollut Res

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The application of hydrogen peroxide (H 2 O 2 ) as a management tool to control Microcystis blooms has become increasingly popular due to its short lifetime and targeted action. H 2 O 2 increases intracellular reactive oxygen species resulting in oxidative stress and subsequently cell death. H 2 O 2 is naturally produced in freshwater bodies as a result of photocatalytic reactions between dissolved organic carbon and sunlight. Previously, some studies have suggested that this environmental source of H 2 O 2 selectively targets for toxigenic cyanobacteria strains in the genus Microcystis. Also, past studies only focused on the morphological and biochemical changes of H 2 O 2 -induced cell death in Microcystis with little information available on the effects of different H 2 O 2 concentrations on growth, esterase activity and membrane integrity. Therefore, this study investigated the effects of non-lethal (40-4000 nM) concentrations on percentage cell death; with a focus on sub-lethal (50 μM) and lethal (275 μM; 500 μM) doses of H 2 O 2 on growth, cells showing esterase activity and membrane integrity. The non-lethal dose experiment was part of a preliminary study. Results showed a general effect of dose and time dependent relationship in all three Microcystis strains post H 2 O 2 treatment. H 2 O 2 resulted in a significant increase in intracellular reactive oxygen species, decreased chlorophyll a content, decreased growth rate and esterase activity. Interestingly, at sub-lethal (50 µM H 2 O 2 treatment), percentage of dead cells in microcystin-producing strains were significantly higher (p<0.05) from non-microcystin producing strains at 72h. These findings further cement our understanding of the influence of H 2 O 2 on different strains of Microcystis and its impact on membrane integrity and metabolic physiology; important to future toxic bloom control programmes.

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Microcystin interferes with defense against high oxidative stress in harmful cyanobacteria

Cited by 61 publications

References 67 publications

Influence of mixed antibiotics on Microcystis aeruginosa during the application of glyphosate and hydrogen peroxide algaecides

Influence of mixed antibiotics on Microcystis aeruginosa during the application of glyphosate and hydrogen peroxide algaecides

The Effect of a Combined Hydrogen Peroxide-MlrA Treatment on the Phytoplankton Community and Microcystin Concentrations in a Mesocosm Experiment in Lake Ludoš

Effects of H2O2 on growth, metabolic activity and membrane integrity in three strains of Microcystis aeruginosa

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