Microcystin-LR label-free immunosensor based on exfoliated graphite nanoplatelets and silver nanoparticles

Zanato, Nicole; Talamini, Lucas; Silva, Tânia Regina; Vieira, Iolanda Cruz

doi:10.1016/j.talanta.2017.07.021

Cited by 15 publications

(14 citation statements)

References 39 publications

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“…The limit of detection (LOD), calculated as three times the standard deviation of the intercept/slope, was 0.053 ng mL À 1 . Table 1 shows a brief comparison of the linear ranges and detection limits of the GCE/MGNnP-PEI-anti-MC-LR and other immunosensors for MC-LR detection, recently published in the literature [12,14,[16][17][18][32][33][34][35]. It can be observed that the LOD of the proposed immunosensor is close to that of previously reported LOD values.…”

Section: Analytical Performance Of Immunosensorsupporting

confidence: 70%

“…It was also found that the highest current suppression was obtained with an incubation time of 40 min, and thus this was considered the ideal time to incubate the MC-LR antigen. In previous studies on electrochemical immunosensors used to detect MC-LR, incubation times ranging from 10 to 240 min have been applied [14,17,33,[36][37][38]. The incubation time selected for the proposed immunosensor is therefore compatible with those cited in the literature.…”

Section: Optimization Of Conditions For Immunosensor Constructionmentioning

confidence: 91%

“…Thin-layer chromatography, high-performance liquid chromatography, enzyme-linked immunosorbent assay, liquid chromatography/mass spectrometry and protein phosphatase inhibition assays are the techniques generally applied to detect low concentrations of MC-LR [12,13]. Recently, electrochemical immunosensors and label-free fluorescent probes for MC-LR detection have been developed as an alternative to traditional techniques, offering low detection limits, no need for sample pretreatment and easy manipulation [14][15][16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

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Label‐free Immunosensor for the Determination of Microcystin‐LR in Water

2020

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This paper describes the development of a label-free electrochemical immunosensor based on polyethyleneimine-stabilized magnetite nanoparticles (MGNnP-PEI) for the determination of microcystin-LR (MC-LR). MGNnP-PEI was immobilized on the glassy carbon electrode (GCE) surface and employed as a platform for anchorage of the anti-MC-LR. Hydroqui-none in solution was used as a redox probe. After optimization, the immunosensor exhibited a linear response to MC-LR concentrations in the range of 0.1 to 500 ng mL À 1 with a calculated detection limit of 0.053 ng mL À 1. The immunosensor was satisfactorily applied in the quantification of MC-LR in a water sample collected from a freshwater lagoon.

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Section: Analytical Performance Of Immunosensorsupporting

confidence: 70%

Section: Optimization Of Conditions For Immunosensor Constructionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

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Label‐free Immunosensor for the Determination of Microcystin‐LR in Water

2020

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“…However, the limited stability of enzyme labels restricts the applications of such sensors. There is therefore an urgent need to develop alternative enzyme-free labels that offer high reproducibility, ease of use, accuracy and portable detection to manage the health risks associated with MC-LR [22,23].…”

Section: Introductionmentioning

confidence: 99%

Colorimetric Method for Sensitive Detection of Microcystin-LR Using Surface Copper Nanoparticles of Polydopamine Nanosphere as Turn-On Probe

et al. 2019

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A novel, facile sensor was further developed for microcystin-LR (MC-LR) determination by visible spectroscopy. Antibody-functionalized SiO2-coated magnetic nanoparticles (Fe3O4@SiO2) and aptamer-functionalized polydopamine nanospheres decorated with Cu nanoparticles (PDA/CuNPs) recognized specific sites in MC-LR and then the sandwich-type composites were separated magnetically. The Cu in the separated composites was converted to Cu2+ ions in solution and turn-on visible absorption was achieved after reaction with bis(cyclohexanone)oxaldihydrazone (BCO) (λmax = 600 nm). There was a quantitative relationship between the spectral intensity and MC-LR concentration. In addition, under the optimum conditions, the sensor turns out to be a linear relationship from 0.05 to 25 nM, with a limit of detection of 0.05 nM (0.05 μg/L) (S/N = 3) for MC-LR. The sensitivity was dependent on the low background absorption from the off-to-on spectrum and label amplification by the polydopamine (PDA) surface. The sensor had high selectivity, which shows the importance of dual-site recognition by the aptamer and antibody and the highly specific color formed by BCO with Cu2+. The bioassay was complete within 150 min, which enabled quick determination. The sensor was successfully used with real spiked samples. These results suggest it has potential applications in visible detection and could be used to detect other microcystin analogs.

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“…Thus, the development of sensitive analytical methods for detection of MC-LR is greatly needed. Low concentrations and different isoforms of MCs in environmental samples present significant analytical challenges (Zanato et al 2017;Zhang et al 2017b).…”

Section: Introductionmentioning

confidence: 99%

Determination of microcystin-LR, employing aptasensors

Bostan

Taghdisi

Bowen

et al. 2018

Biosensors and Bioelectronics

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Cyanobacteria produce toxins such as microcystin-LR (MC-LR), which are associated with potential hepatotoxicity in humans. The detection of cyanobacteria and their toxins in drinking water and sea food is therefore crucial. To date, methods such as high performance liquid chromatography (HPLC), protein phosphatase inhibition assay (PPIA), and Raman spectroscopy have been employed to monitor MC-LR levels. Although these techniques are precise and sensitive, they require expensive instrumentation, well-trained personnel and involve time-consuming processes meaning that their application is generally limited to well-resourced, centralised laboratory facilities. Among the emerging MC-LR detection methods, aptasensors have received great attention because of their remarkable sensitivity, selectivity, and simplicity. Aptamers, also known as "chemical" or "artificial antibodies", serve as the recognition moieties in aptasensors. This review explores the current state-of-the-art of MC-LR aptasensor platforms, evaluating the advantages and, limitations of typical transduction technologies to identify the most efficient detection system for the potentially harmful cyanobacteria associated toxin.

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Microcystin-LR label-free immunosensor based on exfoliated graphite nanoplatelets and silver nanoparticles

Cited by 15 publications

References 39 publications

Label‐free Immunosensor for the Determination of Microcystin‐LR in Water

Label‐free Immunosensor for the Determination of Microcystin‐LR in Water

Colorimetric Method for Sensitive Detection of Microcystin-LR Using Surface Copper Nanoparticles of Polydopamine Nanosphere as Turn-On Probe

Determination of microcystin-LR, employing aptasensors

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