Microdialysis in the estimation of interstitial myocardial neuropeptide Y release

Microdialysis of neuropeptide Y in human muscle tissue

“…Judging from the relations between median and IQR values for each sampling, the intraprobe variation of the relative recovery during the six consecutive samplings was high. This result differs from previous studies where low intraprobe variations were found during microdialysis of NPY (Lambert et al, 1994;Mertes et al, 1993Mertes et al, , 1996Thompson et al, 1995). The reason for the large variation in the present study is unknown, but cannot be explained by degradation of the standard solution since there was no significant difference in NPY concentration between the standard samples obtained at different times.…”

“…Albumin added to the perfusion medium was used to prevent NPY from adhering to the surface of the membrane and tubing as has been suggested previously (Husum et al, 1998;Mertes et al, 1993). A total of 18 MAB probes were used.…”

“…Indeed, only few studies of clinical microdialysis of NPY are available. Most of the in vitro studies show low recovery (Chance et al, 1994;Lambert et al, 1994;Thompson et al, 1995) but Mertes et al (1993) reported a recovery of NPY of 23% with a 2 mm membrane of cellulose and bovine serum albumin added to the perfusate.…”

Determination of myocardial norepinephrine in freely moving rats using in vivo microdialysis sampling and liquid chromatography with dual-electrode amperometric detection

“…Another explanation could be differences in NE between the animal species used in the various studies. However, comparison of the results of Lameris et al [12] and Mertes et al [20] shows that an order of magnitude difference in NE has been reported for the same species. A third explanation for the difference is the use of anesthetized animals in most studies.…”

In vivo microdialysis sampling: theory and applications

“…During the 1990s, the technique has also been adopted for studies in man to investigate free concentrations of various substances in the extravascular space (Stjernstrom et al, 1993;Muller et al, 1995). Numerous reports describing the use of microdialysis technique to study peripheral and brain pharmacokinetics of drugs have recently been reported in animals (Lonnroth et al, 1987;Hurd et al 1988;Sabol and Freed, 1988;Henriksson et al, 1990;Jansson et al, 1990;Scott et al, 1990;Stahle et al, 1990a;Caprioli and Lin, 1991;Deguchi et al, 1991;Deleu et al, 1991;Lunte et al, 1991;Morrison et al, 1991a;Scott et al, 1991;Stahle 1991;Steele et al, 1991;Telting-Diaz et al, 1992;Wong et al, 1992;Yokel et al, 1992;Alavijeh et al, 1993;Mertes et al, 1993;Patsalos et al, 1993;Dykstra et al, 1993;Golden et al, 1993;Kurata et al, 1993;Deleu et al, 1994;O'Connell et al, 1994;Patsalos et al, 1995;Elmquist and Sawchuk, 1997;Yang et al, 1997;Stenken et al 1998) and humans Scheyer et al, 1994a,b;Muller et al, 1998).…”