Microdialysis—principles and applications for studies in animals and man

Ungerstedt, Urban

doi:10.1111/j.1365-2796.1991.tb00459.x

Cited by 747 publications

(471 citation statements)

References 43 publications

Supporting

Mentioning

462

Contrasting

Unclassified

Order By: Relevance

“…43,44 One advantage of this method is that a drug can be delivered into a precise brain region. It is also straightforward to manipulate the dose and time course of drug effects compared to systemic administration.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Physiologically Relevant Changes in Serotonin Resolved by Fast Microdialysis

Yang

Thompson²,

McIntosh³

et al. 2013

ACS Chem. Neurosci.

View full text Add to dashboard Cite

Online microdialysis is a sampling and detection method that enables continuous interrogation of extracellular molecules in freely moving subjects under behaviorally relevant conditions. A majority of recent publications using brain microdialysis in rodents report sample collection times of 20−30 min. These long sampling times are due, in part, to limitations in the detection sensitivity of high performance liquid chromatography (HPLC). By optimizing separation and detection conditions, we decreased the retention time of serotonin to 2.5 min and the detection threshold to 0.8 fmol. Sampling times were consequently reduced from 20 to 3 min per sample for online detection of serotonin (and dopamine) in brain dialysates using a commercial HPLC system. We developed a strategy to collect and to analyze dialysate samples continuously from two animals in tandem using the same instrument. Improvements in temporal resolution enabled elucidation of rapid changes in extracellular serotonin levels associated with mild stress and circadian rhythms. These dynamics would be difficult or impossible to differentiate using conventional microdialysis sampling rates.

show abstract

Section: ■ Results and Discussionmentioning

confidence: 99%

Physiologically Relevant Changes in Serotonin Resolved by Fast Microdialysis

Yang

Thompson²,

McIntosh³

et al. 2013

ACS Chem. Neurosci.

View full text Add to dashboard Cite

show abstract

“…The principles of microdialysis have been described in detail previously (Ungerstedt, 1991;L6nnroth et al, 1987;Morrison et al, 1991). Briefly, microdialysis is based on sampling of analytes from the extracellular space by diffusion through a semipermeable membrane.…”

Section: Microdialysis Proceduresmentioning

confidence: 99%

Measurement of extracellular fluid carboplatin kinetics in melanoma metastases with microdialysis

Blöchl-Daum

Müller²,

Meisinger³

et al. 1996

Br J Cancer

View full text Add to dashboard Cite

Summary Clinical anti-tumour efficacy of anti-cancer drugs is a function of dose intensity, i.e. the concentration -time profile in tumour tissue. Hence, information on drug concentration profiles in tumours is of critical importance but appropriate methods for measurement are lacking. The aim of the present study was to obtain, by microdialysis sampling, concentration -time profiles in a solid tumour (melanoma) of a model anti-cancer drug, carboplatin, and thereby to assess the scope of microdialysis for tumour pharmacokinetic studies in man. Six patients with cutaneous melanoma metastases at the extremities or body trunk, scheduled to receive carboplatin (400 mg m 2 i.v.) were studied. Carboplatin concentrations were monitored in serum, intratumoral and subcutaneous tissue. Calibration of the microdialysis probes was carried out in vitro and in vivo with use of the retrodialysis method. Complete carboplatin concentration vs time profiles in tumour and subcutaneous tissue were obtained. Major pharmacokinetic parameters (maximum concentration, time to maximum concentration, area under the curve, elimination half-life) were calculated for tissues and tumour/ serum concentration ratios for carboplatin were derived. Mean free concentrations of carboplatin in cutaneous melanoma metastases reached only about 50-60% of total serum levels; maximal intratumoral concentrations were 7.6 (±2.0; s.e.m.) Mg ml-', mean concentrations in subcutaneous tissue were similar to those in tumour.The present study demonstrates that microdialysis is a novel tool for measuring drug concentrations in solid tumours in humans in vivo and appears to be a valuable addition for pharmacokinetic/pharmacodynamic studies in oncology.

show abstract

“…Microdialysis is a valuable in vivo tool in basic and clinical research in animals and humans, especially in the neuroscience field [15,31,[41][42][43][44][45][46]. The microdialysis probe monitors continuously the extracellular environment by solute exchange through a porous membrane following chemical gradients.…”

Section: Ce Coupling To Microdialysismentioning

confidence: 99%

Biomedical applications of capillary electrophoresis with laser‐induced fluorescence detection

Páez

Hernández

2001

Biopharm & Drug Disp

View full text Add to dashboard Cite

Capillary electrophoresis (CE) is a high-efficiency analytical technique that has had a great impact as a tool in biomedical research, clinical and forensic practice in the last ten years. Only in one of the applications, the DNA analysis, it has had an explosive exponential growth in the last few years. This impact is expressed in an enormous amount of CE articles and many reviews. The CE advantages with respect to other analytical techniques: the required very small sample volume, rapid analysis, great resolution power and low costs, have made this technique ideal for the analysis of a numerous endogenous and exogenous substances present in biological fluids. The different modes of CE have been coupled to different detection techniques such as UV-absorbance, electrochemical, mass spectrometry and laser-induced fluorescence detection (LIFD) to detect different nature and molecular size separated analytes. This review focuses mostly on the applications of CE-LIFD, to measure drugs and endogenous neuroactive substances such as amino acids and monoamines, especially in microdialysis samples from experimental animals and humans. CE-LIFD trends are discussed: automated faster analysis with capillary array systems, resolution power improvement, higher detection sensitivity, and CE systems miniaturization for extremely small sample volume, in order to make CE easier and affordable to the lab bench or the clinical bed.

show abstract

Microdialysis—principles and applications for studies in animals and man

Cited by 747 publications

References 43 publications

Physiologically Relevant Changes in Serotonin Resolved by Fast Microdialysis

Physiologically Relevant Changes in Serotonin Resolved by Fast Microdialysis

Measurement of extracellular fluid carboplatin kinetics in melanoma metastases with microdialysis

Biomedical applications of capillary electrophoresis with laser‐induced fluorescence detection

Contact Info

Product

Resources

About