Microdissected “cuboids” for microfluidic drug testing of intact tissues

Horowitz, Lisa F.; Rodriguez, A. D.; Yeung, Sung-king Au; Bishop, Kevin W.; Barner, Lindsey A.; Mishra, Gargi; Raman, A.; Delgado, Priscilla; Liu, Jonathan; Gujral, Taranjit S.; Mehrabi, Mehdi; Yang, Moon-Sik; Pierce, Robert H.; Folch, Albert

doi:10.1039/d0lc00801j

Cited by 45 publications

(57 citation statements)

References 73 publications

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“…Recently, a PMMA microfluidic chip to trap cuboidal-shaped microdissected tissues or "cuboids" was engineered [55]. The similarly sized cuboids (400 µm × 400 µm × 400 µm), derived from normal mouse liver and human glioma xenograft tumors (U87), were used and cultured within a collagen hydrogel layer on top of the microwell, with an air interface above and culture medium below (Figure 2b).…”

Section: Combinatorial Drug Screening On a Microscale Samplementioning

confidence: 99%

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Anti-Cancer Drug Screening with Microfluidic Technology

et al. 2021

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The up-and-coming microfluidic technology is the most promising platform for designing anti-cancer drugs and new point-of-care diagnostics. Compared to conventional drug screening methods based on Petri dishes and animal studies, drug delivery in microfluidic systems has many advantages. For instance, these platforms offer high-throughput drug screening, require a small number of samples, provide an in vivo-like microenvironment for cells, and eliminate ethical issues associated with animal studies. Multiple cell cultures in microfluidic chips could better mimic the 3D tumor environment using low reagents consumption. The clinical experiments have shown that combinatorial drug treatments have a better therapeutic effect than monodrug therapy. Many attempts have been made in this field in the last decade. This review highlights the applications of microfluidic chips in anti-cancer drug screening and systematically categorizes these systems as a function of sample size and combination of drug screening. Finally, it provides a perspective on the future of the clinical applications of microfluidic systems for anti-cancer drug development.

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Section: Combinatorial Drug Screening On a Microscale Samplementioning

confidence: 99%

“…3D renderings of device components, as well as micrographs of a device ready for loading (filled with blue dye) and of a device ready for culture (wells filled with red and blue dyes). Reproduced with permission from Ref [55]…”

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confidence: 99%

Anti-Cancer Drug Screening with Microfluidic Technology

et al. 2021

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“…The advantages of microfluidics in such use cases have been demonstrated in applications such as spatial transcriptomics and cancer drug screening. [147][148][149][150] Alternatively, microfluidic methods can be used to perform the sectioning procedure itself. Here, we will focus on describing the microfluidic methods to perform the sectioning procedure at the single cell, dividing cell, and multicellular scales (Figure 12).…”

Section: Sectioningmentioning

confidence: 99%

Microfluidic Surgery in Single Cells and Multicellular Systems

Zhang¹,

Nadkarni²,

Paul³

et al. 2021

Preprint

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Microscale surgery on single cells and small organisms have enabled major advances in fundamental biology and in engineering biological systems. Examples of applications range from wound healing and regeneration studies to the generation of hybridoma to produce monoclonal antibodies. Even today, these surgical operations are often performed manually, but they are labor-intensive and lack reproducibility. Microfluidics has emerged as a powerful technology to control and manipulate cells and multicellular systems at the micro- and nanoscale with high precision. Here, we review the physical and chemical mechanisms of microscale surgery, and the corresponding design principles, applications, and implementations in microfluidic systems. We consider four types of surgical operations: 1) Sectioning, which splits a biological entity into multiple parts, 2) ablation, which destroys part of an entity, 3) biopsy, which extracts materials from within a living cell, and 4) fusion, which joins multiple entities into one. For each type of surgery, we summarize the motivating applications and the microfluidic devices developed. Throughout the review, we highlight existing challenges and opportunities. We hope that this review will inspire scientists and engineers to continue to explore and improve microfluidic surgical methods.

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“…Recently, a PMMA microfluidic chip to trap cuboidal-shaped microdissected tissues or "cuboids" is engineered [53]. The similarly sized cuboids (400 µm×400 µm×400 µm) derived from normal mouse liver and human glioma xenograft tumors (U87) were used and cultured within a collagen hydrogel layer on top of the microwell, with an air interface above and culture medium below.…”

Section: Monodrug Screening On a Macroscale Samplementioning

confidence: 99%

Anti-Cancer Drug Screening with Microfluidic Technology

Monjezi¹,

Rismanian²,

Jamaati³

et al. 2021

Preprint

View full text Add to dashboard Cite

The up-and-coming microfluidic technology is the most promising platform for designing anti-cancer drugs and new point-of-care diagnostics. Compared to conventional drug screening methods based on Petri dishes and animal studies, drug delivery in microfluidic systems has many advantages. For instance, these platforms offer high throughput drug screening, require a small amount of samples, provide an in vivo-like microenvironment for cells, and eliminate ethical issues associated with animal studies. Multiple cell cultures in microfluidic chips could better mimic the 3D tumor environment using low reagents consumption. The clinical experiments have shown that combinatorial drug treatments have a better therapeutic effect than monodrug therapy. So many attempts were performed in this field in the last decade. This review highlights the applications of microfluidic chips in anti-cancer drug screening and systematically categorizes these systems as a function of sample size and combination of drug screening. Finally, it provides a perspective on the future of the clinical applications of microfluidic systems for anti-cancer drug development.

show abstract

Microdissected “cuboids” for microfluidic drug testing of intact tissues

Abstract: A microfluidic platform permits multiple drug testing of uniformly-sized microscale “cuboids” of live tissue with well-preserved microenvironments.

Cited by 45 publications

References 73 publications

Anti-Cancer Drug Screening with Microfluidic Technology

Anti-Cancer Drug Screening with Microfluidic Technology

Microfluidic Surgery in Single Cells and Multicellular Systems

Anti-Cancer Drug Screening with Microfluidic Technology

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