Microemulsions with Levamisole Delivery Systems as Novel Immunomodulating Agents with Potential for Amebiasis Therapies

Ribeiro, Elton Brito; Lanes, Patrícia Kelly Dias; Chaud, Natalina Galdeano Abud; Pessoa, Rafael Souza; Honório-França, Adenilda Cristina; França, Eduardo Luzía

doi:10.1166/sam.2015.2003

Cited by 13 publications

(39 citation statements)

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“…The use of modified release systems for drugs is a viable alternative to modifying the release of a drug or hormone [4,23,24]. In this study we verified that melatonin adsorbed to PEG microspheres had a release profile that was satisfactory in terms of concentration and time (fig.…”

Section: Discussionsupporting

confidence: 66%

The Effect of Melatonin Adsorbed to Polyethylene Glycol Microspheres on the Survival of MCF-7 Cells

França

Honório-França

Fernandes

et al. 2015

Neuroimmunomodulation

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Although melatonin exhibits oncostatic properties such as antiproliferative effects, the oral bioavailability of this hormone is less than 20%. Modified drug release systems have been used to improve the pharmacological efficiency of drugs. These systems can change the pharmacokinetics and biodistribution of the associated drugs. Thus, this study investigated the effect of melatonin adsorbed to polyethylene glycol (PEG) microspheres on MCF-7 human breast cancer cells. The MCF-7 cells were obtained from the American Type Culture Collection. MCF-7 cells were preincubated for 24 h with or without melatonin (100 ng/ml), PEG microspheres or melatonin adsorbed to PEG microspheres (100 ng/ml). Viability, intracellular calcium release and apoptosis in MCF-7 cells were determined by flow cytometry. MCF-7 cells incubated with melatonin adsorbed to PEG microspheres showed a lower viability rate (40.0 ± 8.3 with melatonin adsorbed to PEG microspheres compared to 54.1 ± 7.3 with melatonin; 81.8 ± 12.5 with PEG microsphere and 92.7 ± 4.1 with medium), increased spontaneous intracellular Ca²⁺ release (27.0 ± 8.6 with melatonin adsorbed to PEG microspheres compared to 21.5 ± 13.4 with melatonin; 10.1 ± 5.4 with PEG microsphere and 9.1 ± 5.6 with medium) and increased apoptosis index (51.2 ± 2.7 with melatonin adsorbed to PEG microspheres compared to 36.0 ± 2.1 with melatonin; 4.9 ± 0.5 with PEG microsphere and 3.1 ± 0.6 with medium). The results indicate that melatonin adsorbed to PEG microspheres exerts antitumor effects on human MCF-7 breast cancer cells. However, clinical tests must be performed to confirm the use of melatonin adsorbed to PEG microspheres as an alternative therapy against cancer.

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Section: Discussionsupporting

confidence: 66%

The Effect of Melatonin Adsorbed to Polyethylene Glycol Microspheres on the Survival of MCF-7 Cells

França

Honório-França

Fernandes

et al. 2015

Neuroimmunomodulation

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“…12 The obtained surfactant fractions were 3.5 and 5.5 for SP and TW, respectively, resulting in the surfactant mix ratio of 3.5:5.5:1.0.…”

Section: Surfactant Mixing Ratio Determinationmentioning

confidence: 96%

“…12,26,35 These surfactant contents are necessary for an adequate reduction in the surface tension between the oil and the aqueous phase, allowing the stabilization of the formulation, besides providing the reduced droplet size, in nanometer scale. …”

Section: Development Of Microemulsion Systemsmentioning

confidence: 99%

“…Literature reports that microemulsified systems with a newtonian profile and linear viscosity show stability over a long period, 12,36,37 being a good parameter of evaluation and characterization of stable systems. 42 Other studies that analyzed the stability and rheological behavior of emulsion systems containing Span 83 and Tween 80 obtained systems with non-newtonian flow and higher viscosity.…”

Section: Rheological Characterizationmentioning

confidence: 99%

“…9 The mechanism of microemulsion systems with modified release may change the pharmacokinetics of some orally administered drugs and may reduce their toxicity. [10][11][12] When administered topically, microemulsion has great facility to cross the stratum corneum of the skin, [13][14][15] increasing the skin penetration and allowing its transport to deep layers and underlying tissues.…”

Section: Introductionmentioning

confidence: 99%

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Development, Stability and Antioxidant Activity of Microemulsion Containing Pequi (Caryocar brasiliense Camb.) Oil

Valladão

2018

Rev. Virtual Quim.

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The study of drug delivery systems can potentiate the action of ingredients carried by these systems and the evaluation of their quality parameters offers evidences of the compounds behavior in the formulations. This study aimed to obtain microemulsified formulations containing pequi (Caryocar brasiliense Camb.) oil and to determine its stability, plus its potential antioxidant activity. The pseudoternary phase diagrams were constructed using a mixture of the surfactants sorbitan oleate (Span 80), polysorbate 80 (Tween 80) and cosurfactant, 1-butanol, besides the triglycerides of capric/caprylic acid (Polymol 812) and distilled water. Pequi oil was incorporated in the proportion of 3% (w/w). The selected microemulsions were subjected to centrifugation, 14 days of heating-cooling cycles, and physicochemical analysis (pH, electrical conductivity and refractive index), and then evaluated. Five out of the initial seven formulations remained stable. Therefore, the formulations were subjected to accelerated conditions in triplicate for 90 days. Two microemulsions maintained adequate physical and chemical stability. The hydrodynamic diameter tests showed that two formulations had droplet size in nanometer scale. The rheological profile showed that both formulations presented newtonian behavior and linear viscosity. The analysis of the antioxidant activity showed that the formulations had significant activity and had this activity increased when the pequi oil was added. Concluding, the approved systems kept a remarkable stability and can be used as a modified vehicle to the oil extracted from pequi.

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Interferon‐gamma carrying nanoemulsion with immunomodulatory and anti‐tumor activities

Ribeiro

Marchi

Honório-França

et al. 2019

J Biomedical Materials Res

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The therapeutic administration of cytokines has been introduced aiming to modulate the immune response system, seeking for different approaches to face pathologies such as cancer, auto immune and infectious diseases. The objective of this study was to investigate the effects of a stable oil-in-water (O/W) nanoemulsion system carrying the cytokine Interferon gamma (IFN-γ) on the activity of phagocytes and MCF-7 human breast cancer cells. Nanoemulsions were prepared through ultra-homogenization, and they consisted of distilled water, triglycerides of capric acid/caprylic, sorbitan-oleate, polysorbate 80, and 1-butanol. IFN-γ (100 ng ml −1 ) was incorporated into two O/W nanoemulsion formulations, and these formulations were characterized in terms of their preliminary and accelerated physicochemical stability, rheological properties, droplet size, polydispersity and surface charge. We identified the most optimal IFN-γ nanoemulsion (IFN-γNE2), which remained stable under extreme temperature variations for 90 days, contained an average dose of 97 ng ml −1 of IFNγ and exhibited a biocompatible pH and a relative stable rheological profile. Cell viability and intracellular Ca 2+ release assays conducted showed that IFN-γNE2 reduced the cell viability of MCF-7 cells without affecting the cell viability of phagocytes. Furthermore, IFN-γNE2 was able to induce cellular activity of phagocytes as evidenced by increased intracellular Ca 2+ release in these cells. Our findings on this IFN-γ nanoemulsion suggest that it can be a promising therapeutic agent for immunostimulation and cancer treatment. K E Y W O R D S blood phagocytes, cancer, cell viability, IFN-γ, MCF-7, nanoemulsion

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Microemulsions with Levamisole Delivery Systems as Novel Immunomodulating Agents with Potential for Amebiasis Therapies

Cited by 13 publications

References 0 publications

The Effect of Melatonin Adsorbed to Polyethylene Glycol Microspheres on the Survival of MCF-7 Cells

The Effect of Melatonin Adsorbed to Polyethylene Glycol Microspheres on the Survival of MCF-7 Cells

Development, Stability and Antioxidant Activity of Microemulsion Containing Pequi (Caryocar brasiliense Camb.) Oil

Interferon‐gamma carrying nanoemulsion with immunomodulatory and anti‐tumor activities

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