Microencapsulated plasmids expressing Gn and Gc glycoproteins of Rift Valley Fever virus enhance humoral immune response in mice

Biopolymer‐based Carriers for DNA Vaccine Design

“…Thep DNAc an either be adsorbed on the surface of the nanoparticles (NPs) [101,102] or incorporated within the core. [48,[103][104][105] Further modification of these basic designs can be made by addition of polymeric shells, [51,105] the deposition of NPs in ap olymer matrix, [105][106][107] and preparation of compounded NPs composed of two different material domains within the particle. [108,109] Moreover, metal-based NPs,s uch as gold [110] and iron oxide NPs, [111,112] liposomes, [113][114][115] and ar ange of hydrogels have been used in combination with biopolymers to introduce new functionalities.…”

Biopolymer‐based Carriers for DNA Vaccine Design

“…Thep DNAc an either be adsorbed on the surface of the nanoparticles (NPs) [101,102] or incorporated within the core. [48,[103][104][105] Further modification of these basic designs can be made by addition of polymeric shells, [51,105] the deposition of NPs in ap olymer matrix, [105][106][107] and preparation of compounded NPs composed of two different material domains within the particle. [108,109] Moreover, metal-based NPs,s uch as gold [110] and iron oxide NPs, [111,112] liposomes, [113][114][115] and ar ange of hydrogels have been used in combination with biopolymers to introduce new functionalities.…”

“…This phenomenon can be observed in nature,w here electrostatic interaction has been adopted for condensation of DNA, for example around the histone or protamine proteins,w hich are rich in basic amino acid residues. [120,121] Numerous biopolymers,a mong them chitosan, [101,108,109,[122][123][124][125][126] ePLL, [84,127] poly-l-lysine (PLL), [67,105,[128][129][130][131][132][133] protamine, [76,79,80,[134][135][136][137] polyarginine, [67,90,91,138] and polyspermine [89,[139][140][141] (Table 2), bear intrinsic positive charge,making them excellent candidates as DNAc ondensation agents.…”

Amphiphilic Poly(N-Vinylpyrrolidone) Nanoparticles Loaded with DNA Plasmids Encoding Gn and Gc Glycoproteins of the Rift Valley Fever Virus: Preparation and In Vivo Evaluation

“…Presently, various NPs to encapsulate DNA plasmids have been reported, in particular, liposomes, PLA/PLGA NPs and polysaccharide nano- and microparticles . Recently, we have described polyelectrolyte microcapsules with DNA plasmids encoding Gn and Gc glycoproteins of RVFV which have been prepared by the layer-by-layer (L-by-L) technique …”

“…22 Recently, we have described polyelectrolyte microcapsules with DNA plasmids encoding Gn and Gc glycoproteins of RVFV which have been prepared by the layer-by-layer (L-by-L) technique. 23 The goal of the current study was to develop amphiphilic NPs from PVP derivatives loaded with DNA plasmids encoding the Gn and Gc glycoproteins of RVFV and to estimate a humoral response after administration in mice.…”