Microencapsulation of Bacteriophages Using Membrane Emulsification in Different pH-Triggered Controlled Release Formulations for Oral Administration

Richards, Kerry; Malik, Danish J.

doi:10.3390/ph14050424

Cited by 15 publications

(10 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…More research and scale-up trials are needed to improve the efficiency of phage therapy, to explore the robustness of this experimental approach when applied to modern chicken-management systems, and to assess the phage efficacy versus different Campylobacter field populations in natural breeding conditions. Phages are generally given to animals in feed or water [ 27 ] as simple purified viral suspensions or after microencapsulation [ 35 ]. In particular, the most effective and suitable method for Φ7-izsam and Φ16-izsam administration in poultry on an industrial scale needs to be explored in more details in the near future.…”

Section: Resultsmentioning

confidence: 99%

Bacteriophage Therapy to Reduce Colonization of Campylobacter jejuni in Broiler Chickens before Slaughter

D’Angelantonio

Scattolini

Boni

et al. 2021

Viruses

View full text Add to dashboard Cite

Campylobacteriosis is the most commonly reported gastrointestinal disease in humans. Campybacter jejuni is the main cause of the infection, and bacterial colonization in broiler chickens is widespread and difficult to prevent, leading to high risk of occurrence in broiler meat. Phage therapy represents an alternative strategy to control Campylobacter in poultry. The aim of this work was to assess the efficacy of two field-isolated bacteriophages against experimental infections with an anti-microbial resistant (AMR) Campylobacter jejuni strain. A two-step phage application was tested according to a specific combination between chickens’ rearing time and specific multiplicities of infections (MOIs), in order to reduce the Campylobacter load in the animals at slaughtering and to limit the development of phage-resistant mutants. In particular, 75 broilers were divided into three groups (A, B and C), and phages were administered to animals of groups B and C at day 38 (Φ 16-izsam) and 39 (Φ 7-izsam) at MOI 0.1 (group B) and 1 (group C). All broilers were euthanized at day 40, and Campylobacter jejuni was enumerated in cecal contents. Reductions in Campylobacter counts were statistically significant in both group B (1 log10 colony forming units (cfu)/gram (gr)) and group C (2 log10 cfu/gr), compared to the control group. Our findings provide evidence about the ability of phage therapy to reduce the Campylobacter load in poultry before slaughtering, also associated with anti-microbial resistance pattern.

show abstract

Section: Resultsmentioning

confidence: 99%

Bacteriophage Therapy to Reduce Colonization of Campylobacter jejuni in Broiler Chickens before Slaughter

D’Angelantonio

Scattolini

Boni

et al. 2021

Viruses

View full text Add to dashboard Cite

show abstract

“…Membrane emulsification process used in the present study has previously been used for encapsulation of phage biotherapeutics. Encapsulated phages were shown to be released at defined pH values dependent on the type of pH-responsive polymer used in the formulation and phages shown to withstand gastric acid exposure at pH 1.5 for up to 2h ( Richards and Malik, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Targeted Delivery of Narrow-Spectrum Protein Antibiotics to the Lower Gastrointestinal Tract in a Murine Model of Escherichia coli Colonization

Carpena

Richards²,

González³

et al. 2021

Front. Microbiol.

Self Cite

View full text Add to dashboard Cite

Bacteriocins are narrow-spectrum protein antibiotics that could potentially be used to engineer the human gut microbiota. However, technologies for targeted delivery of proteins to the lower gastrointestinal (GI) tract in preclinical animal models are currently lacking. In this work, we have developed methods for the microencapsulation of Escherichia coli targeting bacteriocins, colicin E9 and Ia, in a pH responsive formulation to allow their targeted delivery and controlled release in an in vivo murine model of E. coli colonization. Membrane emulsification was used to produce a water-in-oil emulsion with the water-soluble polymer subsequently cross-linked to produce hydrogel microcapsules. The microcapsule fabrication process allowed control of the size of the drug delivery system and a near 100% yield of the encapsulated therapeutic cargo. pH-triggered release of the encapsulated colicins was achieved using a widely available pH-responsive anionic copolymer in combination with alginate biopolymers. In vivo experiments using a murine E. coli intestinal colonization model demonstrated that oral delivery of the encapsulated colicins resulted in a significant decrease in intestinal colonization and reduction in E. coli shedding in the feces of the animals. Employing controlled release drug delivery systems such as that described here is essential to enable delivery of new protein therapeutics or other biological interventions for testing within small animal models of infection. Such approaches may have considerable value for the future development of strategies to engineer the human gut microbiota, which is central to health and disease.

show abstract

“…Moreover, in an acidic environment the encapsulate phages were stable, and the T3 phage can survive at low acidic pH. The stability study findings showed a 1 log decrease of phage viability in four weeks in refrigerator storage [ 185 ].…”

Section: Drug Delivery Approaches Via Different Routes To Overcome Antimicrobial Resistancementioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Pharmaceutical Approaches on Antimicrobial Resistance: Prospects and Challenges

et al. 2021

View full text Add to dashboard Cite

The rapid increase in pathogenic microorganisms with antimicrobial resistant profiles has become a significant public health problem globally. The management of this issue using conventional antimicrobial preparations frequently results in an increase in pathogen resistance and a shortage of effective antimicrobials for future use against the same pathogens. In this review, we discuss the emergence of AMR and argue for the importance of addressing this issue by discovering novel synthetic or naturally occurring antibacterial compounds and providing insights into the application of various drug delivery approaches, delivered through numerous routes, in comparison with conventional delivery systems. In addition, we discuss the effectiveness of these delivery systems in different types of infectious diseases associated with antimicrobial resistance. Finally, future considerations in the development of highly effective antimicrobial delivery systems to combat antimicrobial resistance are presented.

show abstract

Microencapsulation of Bacteriophages Using Membrane Emulsification in Different pH-Triggered Controlled Release Formulations for Oral Administration

Cited by 15 publications

References 31 publications

Bacteriophage Therapy to Reduce Colonization of Campylobacter jejuni in Broiler Chickens before Slaughter

Bacteriophage Therapy to Reduce Colonization of Campylobacter jejuni in Broiler Chickens before Slaughter

Targeted Delivery of Narrow-Spectrum Protein Antibiotics to the Lower Gastrointestinal Tract in a Murine Model of Escherichia coli Colonization

Pharmaceutical Approaches on Antimicrobial Resistance: Prospects and Challenges

Contact Info

Product

Resources

About