Microenvironment factors promoting the quality of vitrified cat oocytes

Colombo, Martina; Alkali, Isa Mohammed; Luvoni, Gaia Cecilia

doi:10.1016/j.theriogenology.2022.11.027

Cited by 4 publications

(3 citation statements)

References 91 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our study showed a higher number of presumptive zygotes with a thin zona pellucida that reached the blastocyst stage and started the hatching process. Zona pellucida hardening is a process during which cortical granules are released to prevent polyspermy (Dietzel et al, 2017;Colombo et al, 2023). However, it happens that this process occurs spontaneously already at the IVM stage, often due to inappropriate IVM conditions.…”

Section: Discussionmentioning

confidence: 99%

Cat presumptive zygotes assessment in relation to their development

Kij-Mitka,

Gabryś,

Kochan

et al. 2024

Annals of Animal Science

View full text Add to dashboard Cite

The evaluation of oocytes and zygotes, based on their size, shape and morphology, is a valuable tool for predicting subsequent embryo development. While this assessment is non-invasive and made possible with time-lapse monitoring systems, not all the assessment criteria used for zygotes with pale cytoplasm can be used for domestic cat zygotes, which have dark cytoplasm. In this study, feline presumptive zygotes were evaluated for shape, size, and morphology. Measurements were also made of the diameter of the entire zygote, its cytoplasm, and the zona pellucida. Differences in the dataset were assessed using the generalized linear model (GLM) procedure. While there was no relationship between a combination of the tested parameters with the potential for cleavage, blastocyst development, and hatching, the parameters of the shape and size of the entire oocyte, and of the zona pellucida, were related to the development potential. The results presented in this study indicate that the assessment procedure for human zygotes have to be adjust to be used in the cat model, however the relationship between measurements of the diameter of presumptive feline zygotes and the thickness of zona pellucida with their developmental potential deserve further investigation to optimize assessment of cat presumptive zygotes.

show abstract

Section: Discussionmentioning

confidence: 99%

Cat presumptive zygotes assessment in relation to their development

Kij-Mitka,

Gabryś,

Kochan

et al. 2024

Annals of Animal Science

View full text Add to dashboard Cite

show abstract

“…This event leads to an electro-metabolic uncoupling of the COC as the cation acts by activating the oocytes during the fertilization process [169]. Consequently, this could also influence the mitochondrial biogenesis of COC, essential for oocyte maturation and downstream events such as fertilization and intracytoplasmic sperm injection (ICSI) [169][170][171].…”

Section: Clinical Significance Of Electro-metabolic Coupling Of Cocmentioning

confidence: 99%

Electro-Metabolic Coupling of Cumulus–Oocyte Complex

Del Bianco,

Gentile,

Sallicandro

et al. 2024

IJMS

View full text Add to dashboard Cite

Oocyte–cumulus cell interaction is essential for oocyte maturation and competence. The bidirectional crosstalk network mediated by gap junctions is fundamental for the metabolic cooperation between these cells. As cumulus cells exhibit a more glycolytic phenotype, they can provide metabolic substrates that the oocyte can use to produce ATP via oxidative phosphorylation. The impairment of mitochondrial activity plays a crucial role in ovarian aging and, thus, in fertility, determining the success or failure of assisted reproductive techniques. This review aims to deepen the knowledge about the electro-metabolic coupling of the cumulus–oocyte complex and to hypothesize a putative role of potassium channel modulators in order to improve fertility, promote intracellular Ca2+ influx, and increase the mitochondrial biogenesis and resulting ATP levels in cumulus cells.

show abstract

“…Immature oocytes are usually cryopreserved as cumulus-oocyte complexes (COCs) because cumulus cells (CCs) are crucial for oocyte meiotic resumption and developmental competence [11]. However, IVM is challenging for cryopreserved immature COCs due to membrane damage to CCs [12] and their physical/functional detachment from the oocyte, caused by transzonal projections' sensitivity to cryoprotectants and low temperatures [12,13]. In the last 20 years, the vitrification/warming of immature COCs from adult subjects has been applied to several domestic large (sheep [12,[14][15][16][17][18][19][20][21][22], cow [23][24][25][26][27], buffalo [28][29][30], goat [31], pig [32][33][34][35], horse [36][37][38][39][40][41], donkey [42]) and small (cat [43][44][45][46][47][48], dog [49]) animal species, with various results, depending on intrinsic species-specific oocyte features.…”

Section: Introductionmentioning

confidence: 99%

New Strategies for Conservation of Gentile di Puglia Sheep Breed, an Autochthonous Capital of Millennial Tradition in Southern Italy

Temerario

Monaco

Mastrorocco

et al. 2023

Animals

View full text Add to dashboard Cite

Gentile di Puglia (GdP) is an autochthonous sheep breed of Southern Italy included among ovine breeds threatened by genetic erosion and extinction risk, which have been given attention by local and international institutions, thus emphasizing the need for germplasm conservation actions. In the present study, two assisted reproduction approaches, finalized for GdP conservation, were performed: (1) on-farm reproductive efficiency evaluation, expressed as pregnancy rate (PR), twin pregnancy rate (tPR), and body condition score (BCS), for three consecutive breeding cycles and (2) pre-pubertal lambs’ immature cumulus–oocyte complex (COC) retrieval, vitrification, in vitro maturation (IVM), and assessment of meiotic stage and bioenergetic-oxidative status compared with those of other Italian and European commercial breeds. PR and tPR were progressively reduced over time. In all clinical examination times, BCS was significantly lower in nonpregnant ewes compared with pregnant ones. Fresh GdP pre-pubertal lamb COCs achieved meiotic maturation and showed healthy bioenergetic–oxidative status after IVM. Vitrification reduced the oocyte maturation rate in all groups. However, mature oocytes retained their cytoplasmic maturity, expressed as a mitochondria distribution pattern and activity, indicating promising developmental competence. In conclusion, clinical- and biotechnological-assisted reproduction approaches can support conservation strategies of GdP and other local sheep breeds in Southern Italy.

show abstract

Microenvironment factors promoting the quality of vitrified cat oocytes

Cited by 4 publications

References 91 publications

Cat presumptive zygotes assessment in relation to their development

Cat presumptive zygotes assessment in relation to their development

Electro-Metabolic Coupling of Cumulus–Oocyte Complex

New Strategies for Conservation of Gentile di Puglia Sheep Breed, an Autochthonous Capital of Millennial Tradition in Southern Italy

Contact Info

Product

Resources

About