Microextraction approaches for bioanalytical applications: An overview

Abdel‐Rehim, Mohamed; Pedersen‐Bjergaard, Stig; Abdel-Rehim, Abbi; Lucena, Rafael; Moein, Mohammad Mahdi; Cárdenas, Soledad; Miró, Manuel

doi:10.1016/j.chroma.2019.460790

Cited by 73 publications

(29 citation statements)

References 197 publications

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“…Biological matrices consist of a significant analytical challenge, due to the presence of salts, proteins, and a wide variety of organic compounds [11]. Therefore, a sample preparation procedure is important in most of the cases [12].…”

Section: Introductionmentioning

confidence: 99%

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A proof‐of‐concept of parallel single‐drop microextraction for the rapid and sensitive biomonitoring of pesticides in urine

Mafra

Will

Huelsmann

et al. 2021

J of Separation Science

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In this study, a lab-made parallel single-drop microextraction methodology using the magnetic ionic liquid trihexyltetradecylphosphonium tetrachloromanganate (II) as extraction solvent was developed to determine the pesticides tebuconazole, pendimethalin, dichlorodiphenyltrichloroethane, and dichlorodiphenyldichloroethylene in human urine samples. The experimental setup consisted of a 96-well plate system containing a set of magnetic pins that allowed for the manipulation of up to 96 samples simultaneously, providing an enhanced drop stability compared to traditional single-drop microextraction approaches. The optimal conditions employed 5.38 ± 0.55 mg of extraction solvent, 1.5 mL of diluted urine samples (1:10), extraction time of 130 min, and subsequent dilution in 20 μL of acetonitrile. The method exhibited satisfactory analytical performance, with limits of detection of 7.5 μg/L for all analytes and coefficients of determination higher than 0.9955. Intraday and interday precisions ranged from 3 to 17% (n = 3) and 15 to 18% (n = 9), respectively, with relative recovery of analytes ranging from 70 to 122%. The method proposed was successfully applied in two human urine samples and no sign of the analytes was detected. The results demonstrated that the proposed method allowed for cost-effective and high-throughput methodology to be explored as a valuable tool in bioanalytical applications.

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Section: Introductionmentioning

confidence: 99%

“…The main advantages of SDME are high extraction capacity, low cost, and no carryover effect. However, the drop instability with the use of direct immersion into the sample, limited working temperatures, and long extraction times are drawbacks of this technique [11].…”

Section: Introductionmentioning

confidence: 99%

A proof‐of‐concept of parallel single‐drop microextraction for the rapid and sensitive biomonitoring of pesticides in urine

Mafra

Will

Huelsmann

et al. 2021

J of Separation Science

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“…It is worth highlighting that a classical SPE is nowadays replaced by its miniaturized forms. They, among others, minimize toxic and hazardous organic solvent consumption, reduce the sample volume, and maximize extraction efficiency at the same time [17,18]. One of the miniaturized dispersive SPEs involves the use of magnetic particles modified or coated with different adsorbents [19].…”

Section: Introductionmentioning

confidence: 99%

Application of Magnetic Nanoparticles Coated with Crosslinked Zwitterionic Poly(ionic liquid)s for the Extraction of Oligonucleotides

et al. 2021

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Magnetic nanoparticles coated with zwitterionic poly(ionic liquid)s were applied for dispersive solid-phase extraction of oligonucleotides. The materials were synthesized by miniemulsion copolymerization of ionic liquids and divinylbenzene on magnetic nanoparticles. The functional monomers contain a positively charged imidazolium ring and one of the anionic groups: derivatives of acetate, malonate, or butyl sulfonate ions. Adsorption of unmodified DNA oligonucleotide on obtained materials was possible in ion-exchange (IE) and hydrophilic interactions (HI) mode. The adsorption in IE was possible at low pH and was almost complete. The adsorption in HI mode required the usage of appropriate addition of organic solvent but did not provide full adsorption. Studies on the desorption of the analytes included determining the impact of ammonium acetate concentration and pH and organic solvents addition on the recovery. The material containing acetic fragments as an anionic group was selected for the final procedure with the use of 10 mM ammonium acetate (pH = 9.5)/methanol (50/50, v/v) as an elution solution. The magnetic dispersive solid-phase extraction procedure was tested for the oligonucleotides with various modifications and lengths. Moreover, it was applied to extract DNA oligonucleotide and its synthetic metabolites from enriched human plasma without any pre-purification, with recoveries greater than 80%.

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“…Solid-phase extraction (SPE) has been recognized as an efficient and reduced solvent consumption sample preparation procedure for a wide range of samples, as highlighted by several reviews (Abdel-Rehim et al, 2020;Ashri & Abdel-Rehim, 2011;Wen, Chen, Li, Liu, & Chen, 2014). The selectivity and efficiency of SPE procedures are mainly dependent on the proper choice of the sorbent material.…”

Section: Introductionmentioning

confidence: 99%

Functionalized graphene oxide tablets for sample preparation of drugs in biological fluids: Extraction of ritonavir, a HIV protease inhibitor, from human saliva and plasma using LC–MS/MS

Zohdijamil

Hashemi

Abdel-Rehim

et al. 2021

Biomedical Chromatography

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In this work, graphene oxide-based tablets (GO-Tabs) were prepared by applying a thin layer of functionalized GO on a polyethylene substrate. The GO was functionalized with amine groups (-NH 2 ) by poly(ethylene glycol)bis(3-aminopropyl) terminated (GO-NH 2 -PEG-NH 2 ). The functionalized GO-Tabs were used for the extraction of ritonavir (RTV) in human saliva samples. RTV in plasma and saliva samples was analyzed using LC-MS/MS. Gradient LC system with MS/MS in the positive-ion mode [electrospray ionization (ESI+)] was used. The transitions m/z 721 ! 269.0 and m/z 614 ! 421 were used for RTV and the internal standard indinavir, respectively. This study determined the human immunodeficiency virus protease inhibitor RTV in human saliva samples using functionalized GO-Tab and LC-MS/MS, and the method was validated. The standard calibration curve for plasma and saliva samples was constructed from 5.0 to 2000 nmol L À1 . The limit of detection was 0.1 nmol L À1 , and the limit of quantification was 5.0 nmol L À1 in both plasma and saliva matrices. The intra-and inter-assay precision values were found to be between 1.5 and 5.8%, and the accuracy values ranged from 88.0 to 108% utilizing saliva and plasma samples. The extraction recovery was more than 80%, and the presented functionalized GO-Tabs could be reused for more than 10 extractions without deterioration in recovery.

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Microextraction approaches for bioanalytical applications: An overview

Cited by 73 publications

References 197 publications

A proof‐of‐concept of parallel single‐drop microextraction for the rapid and sensitive biomonitoring of pesticides in urine

A proof‐of‐concept of parallel single‐drop microextraction for the rapid and sensitive biomonitoring of pesticides in urine

Application of Magnetic Nanoparticles Coated with Crosslinked Zwitterionic Poly(ionic liquid)s for the Extraction of Oligonucleotides

Functionalized graphene oxide tablets for sample preparation of drugs in biological fluids: Extraction of ritonavir, a HIV protease inhibitor, from human saliva and plasma using LC–MS/MS

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