Microextraction by packed sorbent and HPLC–PDA quantification of multiple anti-inflammatory drugs and fluoroquinolones in human plasma and urine

Abstract

We developed and validated an analytical method based on microextraction packed sorbent (MEPS) and high-performance liquid chromatography (HPLC) coupled to photodiode array (PDA) detector to simultaneously quantify multiple nonsteroidal anti-inflammatory drugs (NSAIDs) and fluoroquinolones (FLQs), which may provide as combination several adverse reactions in nephrology and neurology. The linearity range from LOQs (0.1 μg/mL) to 10 μg/mL, and LODs values were 0.03 μg/mL for both NSAIDs and FLQs. The validation was performed according to international guidelines and the accuracy was tested measuring the precision, intermediate precision and trueness. The drugs stability was tested under different storage conditions (+4 °C and -20 °C) and after three different cycles of freezing and thawing. The method can be a suitable tool to simultaneously detect a possible association of drugs in human biological samples and provide several potentialities for clinical applications, bioequivalence studies, pharmacodynamics and toxicodynamics of different pharmaceutical dosage forms showing NSAIDs and FLQs.

A modified zeolite/iron oxide composite as a sorbent for magnetic dispersive solid-phase extraction for the preconcentration of nonsteroidal anti-inflammatory drugs in water and urine samples

“…However, the original SPE has undergone numerous modifications to date, mainly related to miniaturization or automatization [20]. Different modalities of miniaturized SPE such as solid-phase microextraction (SPME) [21][22][23][24], stir-bar sorptive extraction (SBSE) [25,26] and microextraction by packed sorbent (MEPS) [27,28] have also been used for the determination of NSAIDs. Magnetic dispersive solid-phase extraction (MDSPE), also called magnetic solid phase extraction (MSPE), uses magnetic or magnetically modified sorbents.…”

Fiberoptic-Coupled Spectrofluorometer with Array Detection as a Process Analytical Chemistry Tool for Continuous Flow Monitoring of Fluoroquinolone Antibiotics

“…Linear range (μg·mL − 1 ) Detection limit (μg·mL − 1 ) Spectrofluorometric [65] 0.02-2.2 0.006-0.016 HPLC-UV [89] 0.15 to 5 0.04-0.08 HPLC [57] 0.003-1.3 0.001 HPLC-fluorescence [90] 0.005-0.1 0.0005-0.005 Capillary electrophoresis [83] 5-20 1.1 and 2.4 Chemiluminescence [77] 1.98 × 10 − 9 0.003-7 HPLC-PDA [91] 0.1-10 0.03 Spectrophotometric [72] 0.5-25 0.084 HPLC [92] 0.0005-1 0.00007 is method 0.25-20 0.035-0.093 International Journal of Analytical Chemistry substances on the proposed method for the determination of ciprofloxacin.…”

Fast quantification of fluoroquinolones in environmental water samples using molecularly imprinted polymers coupled with internal extractive electrospray ionization mass spectrometry

“…6 FQs contaminations create a serious threat to human health due to their harmful effects, such as drug resistant pathogens and causing allergic reactions. [7][8][9][10][11][12] Numerous methods have been developed for the determination of FQs in environmental water, bio-uids, and foodstuffs etc., including electrochemical methods, 13 liquid chromatography tandem mass spectrometry (LC-MS/MS), 14,15 high performance liquid chromatography with ultraviolet detection (HPLC-UV), 16,17 high-performance liquid chromatography coupled to photodiode array (HPLC-PDA), 18 and enzyme immunoassays. 19,20 However, laborious sample pretreatments (e.g., centrifugation and chemical pre-extraction, etc.)…”