Microfabricated Amperometric Cells for Multicomponent Analysis

Liu, Ying; Gyurcsányi, Róbert E.; Jágerszki, Gyula; DeNuzzio, John D.; Lindner, Ernő

doi:10.1002/elan.200904614

Cited by 7 publications

(4 citation statements)

References 52 publications

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“…The functionalized thiol monolayers that are commonly utilized for covalent immobilization of biomolecules onto the SPR chip surfaces can block the charge transfer reaction on the gold surface when it is used for simultaneous amperometric detection [23–25]. However, such problems can be eliminated or minimized by using short alkyl chain thiols, like thioctic acid (ThA), for the immobilization [24–27]. …”

Section: Introductionmentioning

confidence: 99%

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Surface plasmon resonance aided electrochemical immunosensor for CK-MB determination in undiluted serum samples

et al. 2010

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This article presents a simple chronoamperometric immunosensor for the quantitative assessment of creatine kinase MB (CK-MB) in 50 µL undiluted serum samples. The immunosensor consists of gold working and counter electrodes patterned onto a glass chip by thin-film photolithography and an external Ag|AgCl reference electrode. The detection limit (DL) of the chronoamperometric method is 13 ng mL −1 (DL = 2×RMSD/S, where RMSD is the residual mean standard deviation of the measured points around a calibration curve with a slope of S). In spiked serum samples, the response was linear up to 300 ng mL −1 of CK-MB. A surface plasmon resonance (SPR) system with simultaneous electrochemical detection (EC-SPR) aided the development of the sandwich immunoassay. Real-time monitoring of the SPR signal was used to optimize the capture antibody immobilization, CK-MB and detection antibody binding, as well as to minimize the nonspecific adsorption of serum proteins to the sensor surface. The detection antibody has been labeled with alkaline phosphatase (ALP) enzyme for sensitive electrochemical detection. ALP catalyzes the hydrolysis of ascorbic acid phosphate and generates ascorbic acid, which is measured chronoamperometrically. The electrochemical immunoassay for CK-MB was less sensitive to nonspecific adsorption related interferences, had a better detection limit, and required a lower volume of sample than the SPR method.

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Section: Introductionmentioning

confidence: 99%

“…The individual working electrode surfaces will be specifically patterned with capture antibodies for CK-MB, cTr-I and cTr-T. Each assay will use ALP-labeled detection antibodies. A potential planar electrochemical cell design for multi-analyte electrochemical detection was published recently [27]. …”

Section: Introductionmentioning

confidence: 99%

Surface plasmon resonance aided electrochemical immunosensor for CK-MB determination in undiluted serum samples

et al. 2010

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“…43 During the measurement, electrochemical techniques play a fundamental role and can be easily adapted for multiplex immunoassays. [44][45][46] This technique is illustrated in (Figure 2), where the presence of a binded antigen produces an electrochemical change. In theory, any electrode can be adapted for the immobilization of antibodies on its surface.…”

Section: Electro-immuno Sensorsmentioning

confidence: 99%

Electro-Immuno Sensors: Current Developments and Future Trends

Barbosa¹,

Segura²,

Osma³

2017

IJBSBE

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Abbreviations: Iot, internet of things; ELISA, enzyme-linked immuno sorbent assay; WB, western blot; IHC, immunohistochemistry; ICC, immunocytochemistry; FACS, fluorescence-activated cell sorting; IP, immuno precipitation; ELISPOT, enzyme-linked immuno spot; PSA, prostate specific antigen; IDEs, interdigitated electrodes; HPV, human papilloma virus IntroductionBiosensors have been mostly used in the food industry, medical and biological fields, among others, where physical and chemical sensors cannot accurately measure all variables. Biosensors are usually comprised by a biological recognition element and a transducer.1,2 Among the different commercial biosensors, the most common recognition elements are DNA, 3 enzymes, 4 aptamers 5,6 and antibodies.7,8 DNA-based biosensors detect specific analytes by their cleavage and ligation to a functionalized DNA strand.9 Enzymatic biosensors immobilize an enzyme to an electrode to facilitate the transport of electrons from the enzyme to the electrode, produced by specific reactions at the active site of the enzyme.2 Aptamers are chemically synthesized oligonucleotides 10 that provide high specificity and sensitivity towards a specific target. Antibody-based biosensors recognize a specific antigen from the immobilization of a monoclonal or polyclonal antibody.8 In general, transducers vary according to the end user towards which the sensor is thought. Among others, transducers include electrochemical responses, mass changes, optical absorption or transmission, and thermal readings. During the last decades, several immunoassay techniques have appeared as a response to the need of having specific and sensitive tests for antigen recognition. The methods by which the recognition is perform varies from one to another, but the presence of at least one recognition antibody remains as a constant. Among others, the most common immune tests used in laboratories and medical facilities are the enzyme-linked immuno sorbent assay (ELISA), western blot (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), fluorescence-activated cell sorting (FACS), immuno precipitation (IP), and enzyme-linked immuno spot (ELISPOT). Figure 1 shows a general diagram of these immunoassays.ELISA is a widely used technique for the recognition of antibodies' antigens within a sample by means of the biochemical recognition of an enzyme.11 In this technique, specific antibodies are conjugated to an enzyme that catalyzes a colorimetric molecule. Spectrophotometric measurements are used to determine the antigen concentration in the sample. ELISA tests can be performed in a qualitative way, in which the results are positive or negative by statistical methods; or quantitative, in which a standard curve from spectrophotometric measurements can be used to quantify the concentration of the antigen. 12WB is a technique for the detection of specific proteins in a sample through the electrophoretic transfer from a separating gel to a blotting matrix.13 WB uses available monoclonal or polyclonal antibodies for the d...

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“…In one manifestation of an immunodetection system, where a sensor array comprising a large array configuration was used [3], a set of gold electrodes were patterned within recesses in planar Kapton. In one manifestation of an immunodetection system, where a sensor array comprising a large array configuration was used [3], a set of gold electrodes were patterned within recesses in planar Kapton.…”

Section: Immunodetection Of Antigenmentioning

confidence: 99%

Multifunctional Biosensor Development and Manufacture

Vadgama¹

2011

Comprehensive Biotechnology

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Microfabricated Amperometric Cells for Multicomponent Analysis

Cited by 7 publications

References 52 publications

Surface plasmon resonance aided electrochemical immunosensor for CK-MB determination in undiluted serum samples

Surface plasmon resonance aided electrochemical immunosensor for CK-MB determination in undiluted serum samples

Electro-Immuno Sensors: Current Developments and Future Trends

Multifunctional Biosensor Development and Manufacture

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