2006
DOI: 10.1007/s00542-005-0076-9
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Microfabricated fluorescence-activated cell sorter through hydrodynamic flow manipulation

Abstract: In this paper, we present a novel flow manipulation and signal detection scheme for a microfabricated fluorescence-

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Cited by 27 publications
(27 citation statements)
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“…index of refraction, size, dielectric property, etc). The implementation of these sorters often involves external check valves [36, 105], integrated PDMS-based valves [106108], or external syringe pumps [109–110] to achieve flow switching. In one example, Wolff et al attached a high-speed external valve (2.5 ms response time) to the collection outlet to sort rare fluorescent beads from chicken red blood cells (Figure 15).…”
Section: Cell Sorting Techniquesmentioning
confidence: 99%
“…index of refraction, size, dielectric property, etc). The implementation of these sorters often involves external check valves [36, 105], integrated PDMS-based valves [106108], or external syringe pumps [109–110] to achieve flow switching. In one example, Wolff et al attached a high-speed external valve (2.5 ms response time) to the collection outlet to sort rare fluorescent beads from chicken red blood cells (Figure 15).…”
Section: Cell Sorting Techniquesmentioning
confidence: 99%
“…In particular, the reliable generation of disposable microchannel networks constitutes a fundamental requirement of μFACS applications for quality control measures for advanced cell therapies (Cvetković and Dittrich, 2013). Recent publications describe the development of advanced flow cytometry systems involving electroosmotic (Fu et al, 2004) (Figure 4B), dielectrophoretic (Lapizco-Encinas, 2004), magnetic (Pamme and Wilhelm, 2006) (Figure 4C), and hydrodynamic cell sorting application (Bang et al, 2006). While electroosmotic cell sorting allows precise flow switching (Fu, 2002;Chen et al, 2009), only low flow rates of about tens of particles per second can be applied.…”
Section: Flow Cytometry-on-chip and Single Cell Manipulation And Isolmentioning
confidence: 99%
“…2). (Lee et al,2005b), visible focused sample stream This idea was developed experimentally, theoretically and by CFD means by several authors (Bang et al, 2006;Brody et al, 1996;Chein and Tsai, 2004;Dittrich and Schwille, 2003;Hyunwoo et al, 2006;Kruger et al, 2002;Lee et al, 2001a;Lee et al, 2005b;Stiles et al, 2005;Vestad et al, 2004;) and it can be used in conjunction with electrokinetic effects (Dittrich and Schwille, 2003;Schrum et al, 1999;Yamada and Seki, 2005). All proposed applications used initial pre-focusing prior to precise spatial manipulation of the stream making it possible to integrate a whole system of mixers, reactors and separators at one chip (Chung et al, 2003;Goranovic et al, 2001;Klank et al, 2002;Sundararajan et al, 2004).…”
Section: Applications Of Hydrodynamic Focusingmentioning
confidence: 99%