Microfabricated Monolith Columns for Liquid Chromatography. Sculpting Supports for Liquid Chromatography

Regnier, Fred E.

doi:10.1002/(sici)1521-4168(20000101)23:1<19::aid-jhrc19>3.0.co;2-j

Cited by 108 publications

(50 citation statements)

References 18 publications

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“…A standard protocol to fully evaluate and compare col- umns may be of benefit to compare columns fabricated by different laboratories. An area that is expected to grow is that of CEC in microfabricated devices [132±137], offering the possibility of having very well controlled microfabricated channels to perform separations, recently reviewed by Regnier [136]. Fundamental considerations on the generation of EOF and on the separation mechanisms are leading to a better understanding of CEC; however, more research is still required.…”

Section: Discussionmentioning

confidence: 99%

Recent progress in capillary electrochromatography

et al. 2000

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Capillary electrochromatography (CEC) continues to captivate many separation scientists. A remarkable activity is apparent from the numerous publications in the literature using CEC. A review of the most recent progress in CEC is presented herein, covering an extensive fraction of the literature on CEC published from the year 1997 until the beginning of 2000. Most of the recent developments have concentrated on column technology.

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Section: Discussionmentioning

confidence: 99%

Recent progress in capillary electrochromatography

et al. 2000

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“…In the late 1990s, the group of Fred Regnier proposed the use of micro-fabricated pillar array columns as a novel support for liquid chromatography [161][162][163]. Pillar array columns have the advantage that the pillars in the bed can be arranged in a perfectly ordered conformation.…”

Section: Future Perspectives For Column Technologymentioning

confidence: 76%

Evolution and Current Trends in Liquid and Supercritical Fluid Chromatography

Fekete¹,

Perrenoud²,

Guillarme

2014

CCHG

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Abstract:The current trend in high performance liquid chromatography (HPLC) tends toward the achievement of higher separation efficiency and shorter analysis time. Indeed, better performance in LC has become increasingly important in recent years mainly driven by the challenges of either analyzing more complex samples or increasing the numbers of samples per time unit. In the recent development of particle technology, the use of fully porous sub-2 m particles and sub-3 m shell particles have received considerable attention. Beside packed columns, the new generation of silica-based monolithic columns also offers very high separation power. However, to take full advantage of these innovative phases, the chromatographic system has also to be drastically optimized in terms of upper pressure limit and system volume.This revolution in column technology now spreads and covers several modes of liquid chromatography such as reversedphase liquid chromatography (RPLC), hydrophilic interaction liquid chromatography (HILIC), or even supercritical fluid chromatography (SFC). The HILIC and SFC, which can be considered as alternative modes of chromatography, could also be useful to extend the applicability of chromatography towards the analysis of very hydrophilic and lipophilic compounds, respectively.The present review gives an insight about the theory behind the success of current column technology and presents a summary of latest applications, using various modes of one-dimensional chromatography (RPLC, HILIC, SFC). This paper also shows that theoretically expected column efficiency could sometimes be compromised in practical work especially in the case of narrow bore columns.

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“…Although use of microcolumns has been investigated for many years in LC [1][2][3][4][5][6][7][8][9][10], microcolumn-LC (LC) has not been become very popular so far in spite of its features and advantages. The reasons for such a slow popularization may lie in the fact that ancillary techniques such as gradient elution, post-column derivatization and switching valve techniques have not been matured in LC as well as in its undistinguished column efficiency as long as particle-packed columns are used.…”

Section: Introductionmentioning

confidence: 99%