Microfluidic-assisted electrospinning, an alternative to coaxial, as a controlled dual drug release system to treat inflammatory arthritic diseases

Vasconcelos, Filipa; Lima, Antônio Carlos Pedroso de; Bonani, Walter; Silva, Catarina S.; Reis, Rui L.; Motta, Antonella; Migliaresi, Claudio; Martins, Albino; Neves, Nuno M.

doi:10.1016/j.msec.2021.112585

Cited by 13 publications

(2 citation statements)

References 48 publications

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“…The eFMs are easy to handle, mechanically stable, and present an interconnected 3D structure. 27 Moreover, eFMs show a large surface area and high porosity, allowing extensive cellular adhesion and migration, as previously demonstrated by our group. 28−30 This work aims to study the effect of ECM density on TEC survival and proliferation.…”

Section: Introductionsupporting

confidence: 73%

“…As such, herein, electrospun fibrous meshes (eFMs) were used as a carrier to overcome these limitations. The eFMs are easy to handle, mechanically stable, and present an interconnected 3D structure . Moreover, eFMs show a large surface area and high porosity, allowing extensive cellular adhesion and migration, as previously demonstrated by our group. − …”

Section: Introductionsupporting

confidence: 54%

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The Influence of Feeder Cell-Derived Extracellular Matrix Density on Thymic Epithelial Cell Culture

Silva

Pinto

Correia-Neves

et al. 2023

ACS Biomater. Sci. Eng.

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The thymus is responsible for the selection and development of T cells, having an essential role in the establishment of adaptive immunity. Thymic epithelial cells (TECs) are key players in T cell development interacting with thymocytes in the thymic 3D environment. Feeder-layer cells have been frequently used as platforms for the successful establishment of TEC cultures. Nevertheless, the role of the feeder cell-derived extracellular matrix (ECM) on TEC cultures was not previously reported. Therefore, this work aimed at assessing the effect of the ECM produced by feeder cells cultured at two different densities on the establishment of TEC culture. Due to the high surface area and porosity, electrospun fibrous meshes were used to support ECM deposition. The feeder cell-derived ECM was efficiently recovered after decellularization, maintaining the composition of major proteins. All the decellularized matrices were permeable and showed an increase in surface mechanical properties after decellularization. TEC cultures confirmed that the ECM density impacts cellular performance, with higher densities showing a decreased cellular activity. Our findings provide evidence that feeder cellderived ECM is a suitable substrate for TEC culture and can potentially be applied in thymus bioengineering.

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Section: Introductionsupporting

confidence: 73%

Section: Introductionsupporting

confidence: 54%

The Influence of Feeder Cell-Derived Extracellular Matrix Density on Thymic Epithelial Cell Culture

Silva

Pinto

Correia-Neves

et al. 2023

ACS Biomater. Sci. Eng.

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Engineering free-standing electrospun PLLCL fibers on microfluidic platform for cell alignment

Yildirim-Semerci,

Arslan-Yildiz

2024

Microfluid Nanofluid

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Here, a PLLCL-on-chip platform was developed by direct electrospinning of poly (L-lactide-co-ε-caprolactone) (PLLCL) on polymethyl methacrylate (PMMA) microfluidic chips. Designed microchip provides the electrospinning of free-standing aligned PLLCL fibers which eliminates limitations of conventional electrospinning. Besides, aligned fiber structure favors cell alignment through contactless manipulation. Average fiber diameter, and fiber alignment was evaluated by SEM analyses, then, leakage profile of microchip was investigated. 3D cell culture studies were conducted using HeLa and NIH-3T3 cells, and nearly 85% cell viability was observed in PLLCL-on-chip for 15 days, while cell viability of 2D control started to decrease after 7 days based on Live dead and Alamar Blue analyses. These findings emphasize biocompatibility of PLLCL-on-chip platform for 3D cell culture and its ability to mimic extracellular matrix (ECM). Immunostaining results prove that PLLCL-on-chip platform favors the secretion of ECM proteins compared to control groups, and cytoskeletons of cells were in aligned orientation in PLLCL-on-chip, while they were in random orientation in control groups. Overall, these results demonstrate that the developed platform is suitable for the formation of various 3D cell culture models and a potential candidate for cell alignment studies. Graphical Abstract

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Electrospinning process: fiber composition and applications

El kalaaoui,

Bili,

Boukhriss

et al. 2024

Synthetic and Mineral Fibers, Their Composites and Applications

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Microfluidic-assisted electrospinning, an alternative to coaxial, as a controlled dual drug release system to treat inflammatory arthritic diseases

Cited by 13 publications

References 48 publications

The Influence of Feeder Cell-Derived Extracellular Matrix Density on Thymic Epithelial Cell Culture

The Influence of Feeder Cell-Derived Extracellular Matrix Density on Thymic Epithelial Cell Culture

Engineering free-standing electrospun PLLCL fibers on microfluidic platform for cell alignment

Electrospinning process: fiber composition and applications

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