Microfluidic-based exosome isolation and highly sensitive aptamer exosome membrane protein detection for lung cancer diagnosis

Zhao, Liang; Wang, Hong; Jun, Fu; Wu, Xia; Liang, Xiao-ye; Liu, Xue-yu; Wu, Xue; Cao, Liangliang; Xu, Zhenyu; Dong, Ming

doi:10.1016/j.bios.2022.114487

Cited by 74 publications

(40 citation statements)

References 34 publications

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“…Exo-CMDS is capable of isolating and purifying exosomes in a single step and has a highly qualified yield with an ideal exosome concentration of 5.1 × 10 9 particles per ml from trace blood samples (less than 300 μl) in only 8 minutes. 99 Urine is the second most important biological fluid after saliva because it can be collected quickly, painlessly, without special procedures or equipment, and because it can be obtained in large quantities. The best thing about using urine instead of tissue or blood is that taking urine isn't as painful as taking tissue or blood.…”

Section: Other Biological Fluidsmentioning

confidence: 99%

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Tailored point-of-care biosensors for liquid biopsy in the field of oncology

et al. 2023

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Section: Other Biological Fluidsmentioning

confidence: 99%

Section: Emerging Novel Poc Based Approach For Detection Of Liquid Bi...mentioning

confidence: 99%

Tailored point-of-care biosensors for liquid biopsy in the field of oncology

et al. 2023

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“…The centrifugal microfluidic disc system (Exo-CMDS) is proposed by Zhao et al (2022) for the isolation of exosomes. The automated centrifugal microfluidic disc system is combined with a functionalized membrane to form Exo-CMDS.…”

Section: Capture By the Centrifugal Microfluidic Disc Systemmentioning

confidence: 99%

Recent developments in isolating methods for exosomes

Gao

et al. 2023

Front. Bioeng. Biotechnol.

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Exosomes are the smallest extracellular vesicles that can be released by practically all cell types, and range in size from 30 nm to 150 nm. As the major marker of liquid biopsies, exosomes have great potential for disease diagnosis, therapy, and prognosis. However, their inherent heterogeneity, the complexity of biological fluids, and the presence of nanoscale contaminants make the isolation of exosomes a great challenge. Traditional isolation methods of exosomes are cumbersome and challenging with complex and time-consuming operations. In recent years, the emergence of microfluidic chips, nanolithography, electro-deposition, and other technologies has promoted the combination and innovation of the isolation methods. The application of these methods has brought very considerable benefits to the isolation of exosomes such as ultra-fast, portable integration, and low loss. There are significant functional improvements in isolation yield, isolation purity, and clinical applications. In this review, a series of methods for the isolation of exosomes are summarized, with emphasis on the emerging methods, and in-depth comparison and analysis of each method are provided, including their principles, merits, and demerits.

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“…Despite fabrication challenges, microfluidic systems have the potential to integrate different mechanical parameters into one practical, inexpensive, and efficient device. The centrifugal microfluidic disc with functionalized membranes proposed by Zhao et al 35 is an example of such a multiparametric system that can isolate exosome from whole blood samples. The disc is composed of engraved units that communicate with each other by capillary channels and consists of two polycarbonate (PC) layers with the bottom layer engraved with six hydrophilically modified functional units (a loading chamber, a chamber for whole blood separation, a mixing chamber, an input for exosome loading buffer, an exosome isolating buffer chamber, and a commutator unit).…”

Section: Isolation Techniquesmentioning

confidence: 99%

Multiparametric Biosensors for Characterizing Extracellular Vesicle Subpopulations

Shaabani

Meira

Marcet‐Palacios

et al. 2023

ACS Pharmacol. Transl. Sci.

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Extracellular vesicles (EVs) are an important intercellular communication conduit for cells that have applications in precision therapy and targeted drug delivery. Small EVs, or exosomes, are a 30−150 nm phospholipid-encased subpopulation of EVs that are particularly difficult to characterize due to their small size and because they are difficult to isolate using conventional methods. In this review, we discuss some recent advances in exosome isolation, purification, and sensing platforms using microfluidics, acoustics, and size exclusion chromatography. We discuss some of the challenges and unanswered questions with respect to understanding exosome size heterogeneity and how modern biosensor technology can be applied to exosome isolation. In addition, we discuss how some advancements in sensing platforms such as colorimetric, fluorescent, electronic, surface plasmon resonance (SPR), and Raman spectroscopy may be applied to exosome detection in multiparametric systems. The application of cryogenic electron tomography and microscopy to understanding exosome ultrastructure will become vital as this field progresses. In conclusion, we speculate on some future needs in the exosome research field and how these technologies could be applied.

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Microfluidic-based exosome isolation and highly sensitive aptamer exosome membrane protein detection for lung cancer diagnosis

Cited by 74 publications

References 34 publications

Tailored point-of-care biosensors for liquid biopsy in the field of oncology

Tailored point-of-care biosensors for liquid biopsy in the field of oncology

Recent developments in isolating methods for exosomes

Multiparametric Biosensors for Characterizing Extracellular Vesicle Subpopulations

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