Microfluidic-based generation of functional microfibers for biomimetic complex tissue construction

Zuo, Yicong; He, Xiao-Heng; Yang, You; Wei, Dan; Sun, Jing; Zhong, Meiling; Xie, Rui; Fan, Hongsong; Zhang, Xingdong

doi:10.1016/j.actbio.2016.04.036

Cited by 79 publications

(97 citation statements)

References 28 publications

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“…Matrigel Hydrogel HOS, 143B -pLKO.1 shsFRP2 vector [73] 143B, MG-63, U2OS, hFOB1.19 -Paris polyphylla ethanol extract [75] MG-63 -miR-29b-1 overexpression [76] K7M2 -Bromodomain inhibitor JQ1 [77] Collagen Hydrogel U2OS -Kinase inhibitor PI103 [43] Type I MG-63 -Tyrosin phosphorylation inhibitor (ACM-14) [91] LM8 -Pazopanib [96] Sponge hFOB 1.19 (osteoblasts) PC3, LNCaP siRNA-based gene knockdown [97] Matrigel/Collagen Hydrogel MOS, U2OS, 143B, ZK58, KPD, SaOS-2 Trametinib, AZD8330, and TAK-733 kinase inhibitors [79] MOS, U2OS, 143B, ZK58, KPD -siAven [78] Silk Fibroin Sponge 143.98.2, SaOS-2, U2OS -Doxorubicin, cisplatin [108] U2OS Bone marrow fibroblasts, HUVECs MAB208 (anti-IL-8), Avastin (anti-VEGF-A) [109] MG-63 MDA-MB-231 Doxorubicin [110] MG-63 MDA-MB-231, MSCs Paclitaxel [111] Chitosan Sponge MG-63, MNNG -Doxorubicin [139] Alginate Hydrogel MG-63 -Tetracycline hydrochloride [115] Fibers MG-63 HUVECs - [116] Spheres Dunn, LM8 NF-кB decoy oligodeoxy-nucleotide [124] Synthetic Biomaterials…”

Section: Natural Biomaterialsmentioning

confidence: 99%

“…The targeted drug delivery decreased the metastatic cell population, while the normal metabolic activity of osteoblasts and bone mineralization remained, demonstrating that this 3D platform is suitable as a high-throughput screening system for cancer drug discovery and development. [115][116][117][118] The cross-linking of HAp into alginate-based hydrogel combined with gelatin microspheres encapsulating tetracycline hydrochloride improved the mechanical features of the scaffold in a manner that potentiate its application in bone tissue regeneration. [111] The main difference with the above-mentioned study is the construction of a co-culture to more faithfully recapitulate the migration of breast cancer cells to bone microenvironment.…”

Section: Natural Biomaterialsmentioning

confidence: 99%

“…[111] The main difference with the above-mentioned study is the construction of a co-culture to more faithfully recapitulate the migration of breast cancer cells to bone microenvironment. [116] The mechanical properties of this composite material allowed the fabrication of a biomimetic osteon-like structure through a controllable double-layer hollow microfiber, replicating the interaction between endothelial cells that constitute the vascular vessel and bone-derived cells. Metastatic cells showed to chemotactically invade bone microenvironment, where an increase of its population, in contrast with the decrease of osteoblasts population, was observed.…”

Section: Natural Biomaterialsmentioning

confidence: 99%

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Three‐Dimensional Osteosarcoma Models for Advancing Drug Discovery and Development

Monteiro

Custódio

Mano

2018

Advanced Therapeutics

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Osteosarcoma (OS) is the most common primary malignant tumor of bone that mainly affects children and adolescents. The currently available therapies are not effective and the search for new OS anticancer drugs is extremely urgent. Understanding the mechanisms that underlie the tumor progression, invasion, and metastasis is an essential step toward effective cancer therapies. Tissue engineering has given a great contribution to the development of reliable and cost-effective platforms for drug screening and validation through 3D in vitro models that more faithfully mimic the in vivo pathophysiology than conventional 2D models. The progress in the field of functional and biomimetic biomaterials in the development of 3D tissue models has provided tools for a close recapitulation of the highly complex and dynamic tumor microenvironment. This review focuses on the most recent advances in 3D in vitro osteosarcoma models, highlighting the crucial role of the extracellular matrix and stromal cells in tumor progression, how they contribute to drug resistance and disease prevalence, and the future pathways toward an effective and personalized model for drug screening and validation.

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Section: Natural Biomaterialsmentioning

confidence: 99%

Section: Natural Biomaterialsmentioning

confidence: 99%

Section: Natural Biomaterialsmentioning

confidence: 99%

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Three‐Dimensional Osteosarcoma Models for Advancing Drug Discovery and Development

Monteiro

Custódio

Mano

2018

Advanced Therapeutics

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“…After removing the alginate hydrogel shell, linear microorganoids composed of two types of cells were formed. Zuo et al (2016) prepared composite, double-layered hollow microfibers made of alginate and methacrylated gelatin, which were used to simulate complex tissues. One of the advantages of these systems is the ability to physically separate the inner and outer environments using the thin alginate hydrogel wall to retain specific substances or cells in the restricted linear core regions.…”

Section: Production Of Anisotropic and Complex Bersmentioning

confidence: 99%

Multiphase Microfluidic Processes to Produce Alginate-Based Microparticles and Fibers

Yamada

Seki

2018

Journal of Chemical Engineering of Japan

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With the recent developments in micro uidic instruments and devices, various types of micrometer-sized materials have been produced by employing multiphase ow patterns formed in the microchannel. In particular, microparticles and micro bers, which are compatible with biomolecule incorporation or living cell encapsulations, have been gaining signi cant attention as new tools for biochemical analysis, cellular physiological studies, tissue engineering, cell transplantation, and controlled drug delivery. Herein, we introduce recent developments in micro uidic systems to produce alginate-based hydrogel microparticles and micro bers. By utilizing droplet dispersions either in equilibrium or nonequilibrium states, or by employing parallel laminar ows, microengineered functional materials that are di cult to generate using conventional devices and operations can be obtained. New and interesting multiphase phenomena are reviewed, together with the pros and cons of these systems and their applications. Furthermore, the fundamentals of multiphase micro uidics and the materials used to prepare particles and bers are brie y introduced.

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“…Methacrylated gelatin(GelMA)/alginate double-layer hollow composite fiber was fabricated using a cylindrical concentric-flow microchannel with three inlets [81]. Incorporation of GelMA increased the mechanical strength and decreased the swelling ratio of the fiber.…”

Section: Composite Fiber Fabrication Using Microfluidic Methodsmentioning

confidence: 99%

Aligning carbon nanofibers in PCL microfibers using microfluidic method: An approach to efficiently improve electrical conductivity and mechanical strength

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In this thesis work, electrically conductive CNF/PCL composite fibers were fabricated using the microfluidic method. The fibers were made with different content levels of CNFs and flow rate ratios between the core and sheath fluids. The electrical conductivity and tensile properties of these fibers were then investigated. A cross-flow geometry microfluidic channel with four chevron-shaped grooves as shaping elements was designed for microfluidic fiber fabrication. A CNC micro-milling machine was used to create the PMMA master molds, the surfaces of which were further smoothened by chloroform vapor. PDMS microchannel was prepared by molding replication based on the micro-machined PMMA master molds. Using the as-fabricated PDMS microchannel, electrically conductive CNF/PCL composite fibers were successfully fabricated using the microfluidic method. It was found that at a CNF concentration of 3 wt.%, the electrical conductivity of the composite fiber increased to 1.11 S/m, which was around 10 15 times of the electrical conductivity of the pure PCL. The yield strength, Young's Modulus and ultimate strength of the 3 wt.% CNF/PCL composite fiber increased relative to the pure PCL fiber by a factor of 1.72, 2.88, and 1.23, respectively. Further increasing the content of CNF, the electrical conductivity increased slightly, while the tensile strength dropped sharply due to the agglomeration of CNF. Additionally, the results showed that the microfluidic could be considered as an effective method to align CNFs along the fiber in the longitudinal direction. The alignment of the CNFs showed a positive effect on the electrical conductivity and tensile strength.

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Microfluidic-based generation of functional microfibers for biomimetic complex tissue construction

Cited by 79 publications

References 28 publications

Three‐Dimensional Osteosarcoma Models for Advancing Drug Discovery and Development

Three‐Dimensional Osteosarcoma Models for Advancing Drug Discovery and Development

Multiphase Microfluidic Processes to Produce Alginate-Based Microparticles and Fibers

Aligning carbon nanofibers in PCL microfibers using microfluidic method: An approach to efficiently improve electrical conductivity and mechanical strength

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