Microfluidic-based isolation of circulating tumor cells with high-efficiency and high-purity

Wu, Feng; Kong, Xuemin; Liu, Yixuan; Wang, Shuli; Chen, Zhong; Hou, Xu

doi:10.1016/j.cclet.2024.109754

Cited by 4 publications

(1 citation statement)

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“…Different markers have different significance, such as the fact that by combining liquid-like polymer chains and anti-EpCAM mechanisms, the non-specific binding of proteins and leukocyte pairs can be effectively attenuated, improving the capture efficiency of CTCs [ 30 ]. Label-independent isolation technologies based on physical principles have also been widely investigated for the enrichment of CTCs [ 31 , 32 ]. These technologies isolate CTCs based on differences in size, density, deformability, and dielectric properties ( Figure 1 ).…”

Section: Introductionmentioning

confidence: 99%

Novel Isolating Approaches to Circulating Tumor Cell Enrichment Based on Microfluidics: A Review

Qiao,

Teng,

Liu

et al. 2024

Micromachines

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Circulating tumor cells (CTCs), derived from the primary tumor and carrying genetic information, contribute significantly to the process of tumor metastasis. The analysis and detection of CTCs can be used to assess the prognosis and treatment response in patients with tumors, as well as to help study the metastatic mechanisms of tumors and the development of new drugs. Since CTCs are very rare in the blood, it is a challenging problem to enrich CTCs efficiently. In this paper, we provide a comprehensive overview of microfluidics-based enrichment devices for CTCs in recent years. We explore in detail the methods of enrichment based on the physical or biological properties of CTCs; among them, physical properties cover factors such as size, density, and dielectric properties, while biological properties are mainly related to tumor-specific markers on the surface of CTCs. In addition, we provide an in-depth description of the methods for enrichment of single CTCs and illustrate the importance of single CTCs for performing tumor analyses. Future research will focus on aspects such as improving the separation efficiency, reducing costs, and increasing the detection sensitivity and accuracy.

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Section: Introductionmentioning

confidence: 99%