Microfluidic biochip for chemiluminescent detection of allergen-specific antibodies

Heyries, Kevin A.; Loughran, Michael; Hoffmann, Daniel; Homsy, Alexandra; Blum, Loı̈c J.; Marquette, Christophe A.

doi:10.1016/j.bios.2008.02.025

Cited by 82 publications

(51 citation statements)

References 44 publications

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“…It was found that for low values of diffusivity (\10 -10 m 2 /s) the detection performance is limited by transport process, whereas at higher values the performance is dictated by reaction kinetics. For the conditions used in this study [diffusion coefficient *10 -11 m 2 /s and inlet velocity (*10 lm/s)] and also in typical protein detection system (Heyries et al 2008) the sensing performance of the device fall in transport-limited regime. Therefore, increasing the mass transport is the key for enhancing the detection time of these devices which is also consistent with the results obtained in literature (Friedrich et al 2008;Kim and Zheng 2008).…”

Section: Influence Of Convection and Diffusionmentioning

confidence: 99%

Numerical analysis of a magnetic nanoparticle-enhanced microfluidic surface-based bioassay

Munir

Wang

et al. 2009

Microfluid Nanofluid

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An innovative magnetic nanoparticle (MNP)-based strategy for enhancing the dynamics and kinetics of surface-based antigen-antibody binding in a microfluidic platform is presented in this study. Finite element technique was employed for quantifying the effect of convection, diffusion, reaction and magnetic field on the detection performance of surface-based bio-assay. It was identified that diffusion is rate limiting when compared with reaction and convection. In order to reduce the detection time, increasing diffusion transport or in general bringing more target antigen towards the surface-bound antibody will be most effective. A novel and simple strategy based on tagging the antigen with MNPs was demonstrated using the numerical model. It was found that local concentration of antigen-MNP complex in the vicinity of sensing surface was increased when magnetic field was used. Different configurations of magnetic field around the microchannel for focusing target antigen towards the sensing surface were simulated and the most optimized configuration was identified. Furthermore, it was quantitatively demonstrated that MNP enhanced the surface-binding kinetics and reduced the detection time of target antigen by almost 42%. Moreover, when compared with physical means of reducing diffusion barrier, MNP-based detection was 35% more efficient. Overall, MNPs enhanced the mass transport of target antigen towards sensing surface which resulted in considerable reduction in detection time. The simulations performed using the developed model will not only help to investigate a wide range of design parameters but also provide generic strategy that can be exploited at the concept stage for designing, optimizing and developing efficient and fast small-scale surface-based bioassays.

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Section: Influence Of Convection and Diffusionmentioning

confidence: 99%

Numerical analysis of a magnetic nanoparticle-enhanced microfluidic surface-based bioassay

Munir

Wang

et al. 2009

Microfluid Nanofluid

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“…All liquid handling and sample processing were fully automated, and one analysis was carried out in milk within less than 5 min. Heyries et al 103 developed a fully automated and integrated microfluidic device for allergy agnosis. Three different proteins (blactoglobulin, peanut lectin and human IgG) were immobilized via a macromolecules of polydimethylsiloxane elastomer, and used as a capturing agent for the detection of specific antibodies.…”

Section: ·1·3 Microfluidic Biochipmentioning

confidence: 99%

Chemiluminescence Platforms in Immunoassay and DNA Analyses

Fan

Cao

et al. 2009

ANAL. SCI.

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IntroductionCL is the production of electromagnetic radiation by a chemical reaction. The CL intensity is directly proportional to the concentration of a limiting reactant involved in the CL reaction. CL has been exploited for its use in a wide range of applications due to its high sensitivity, wide calibration range, and suitability for miniaturization. Protein and DNA analyses have recently attracted much attention. Many different types of techniques, such as electrochemical, optical, radiochemical, and piezoelectronic methods have been applied for protein and DNA analyses. To provide an overview of CL detection assays for DNAs and proteins, we have summarized their essential techniques. Monoplex and multiplex assays are described, and their current reports are intended to provide an overview of the CL techniques currently employed in numerous laboratories. Monoplex AssayA monoplex assay aims to measure the presence of a single analyte in any given sample, in which nanoparticle, DNA enzyme, horseradish peroxidase (HRP), alkaline phosphatase (AP) and acridinium ester are commonly used as labels. 2·1 Nanoparticle-based CL techniquesNanoparticles offer excellent prospects for DNA detection and immunoassay, owing to their many attractive properties. Compared to existing labels, nanoparticles are more stable and cheaper, and easier to be purified. In addition, they indicate faster binding kinetics with high sensitivity and a high reaction rate for many types of monoplex assays, ranging from immunoassays to DNA detection. 2·1·1 Gold nanoparticlesColloidal gold labels are ideal in biotechnological systems for several reasons. Firstly, they can be readily prepared in a wide range of sizes, from approximately 2 nm to above 100 nm. Secondly, the biochemical activity of the labeled biomolecules can be retained when colloidal gold is coupled to the biomolecules and, thirdly, colloidal gold labels can be easily visualized as dense structures within biological entities using transmission electron microscopy. As a biological tag, colloidal gold has been employed in several CL detection systems. 2·1·1·1 Gold nanoparticle-based CL immunoassay. The Au 3+ -luminol reaction, in which 10 -9 M Au 3+ ions can be sensitively detected, is a classic CL reaction. Thousands of Au atoms are contained within the gold nanoparticles; for example, 2.3 ¥ 10 5 Au atoms are theoretically contained in a 20-nm spherical gold particle and, consequently, picomolar detection limits can be attained by employing oxidative gold metal dissolution in acidic solutions. Our group 1,2 reported a sensitive CL immunoassay based on a colloidal gold label after oxidative gold metal dissolution (using 0.01 M HCl-0.5 M NaCl-0.5 mM Br2), using a simple and sensitive luminol-CL reaction (Fig. 1). Reviews Chemiluminescence Platforms in Immunoassay and DNA AnalysesAiping FAN,* Zhijuan CAO,* Huan LI,* Masaaki KAI,** and Jianzhong LU* † *School of Pharmacy, Fudan University, 138 Yixueyuan Road, Shanghai 200032, China ** Faculty of Pharmaceutical Sciences, Graduate Scho...

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“…6,7 For these analytical purposes, fluorescence, electrochemical, chemiluminescence (CL) and label-free microarray read-out systems have been developed. [8][9][10][11][12][13][14] CL microarrays use the effect of light emission caused by a chemical reaction with the assistance of an enzyme label. In contrast to fluorescence, there is no background signal, neither from the light source nor from light scattering of the matrix.…”

Section: Introductionmentioning

confidence: 99%

Quantification of E. coli DNA on a Flow-through Chemiluminescence Microarray Readout System after PCR Amplification

Donhauser

Seidel

2009

ANAL. SCI.

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We report on a hybridization assay using DNA microarrays for the quantification of amplification products of the uidA gene of E. coli. Using the stopped-PCR strategy, the amplified target DNA was strongly dependent on the applied gene copies. The quantification was carried out by a flow-through chemiluminescence microarray readout system. The DNA microarrays were based on a poly(ethylene glycol)-modified glass substrate. The probes on the surface were 18 or 25 nucleotides long and the quantified PCR product was 60 nucleotides. The amplification was stopped after 25 cycles; at this point amplification was in the middle of the logarithmical phase, and the spread between different DNA starting concentrations reached the maximum. A conjugate of streptavidin and horseradish peroxidase (HRP) bound to the biotinylated strands on the microarray surface and catalyzed the reaction of luminol and hydrogen peroxide. The generated light emission was recorded by a sensitive charge-coupled device (CCD) camera. The detection limit for the gene uidA (b-galactosidase) of E. coli was 1.1 ¥ 10 5 copies/mL. This system allowed for a sensitive detection and quantification of E. coli in a concentration range from 10 6 to 10 9 copies/mL.

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Microfluidic biochip for chemiluminescent detection of allergen-specific antibodies

Cited by 82 publications

References 44 publications

Numerical analysis of a magnetic nanoparticle-enhanced microfluidic surface-based bioassay

Numerical analysis of a magnetic nanoparticle-enhanced microfluidic surface-based bioassay

Chemiluminescence Platforms in Immunoassay and DNA Analyses

Quantification of E. coli DNA on a Flow-through Chemiluminescence Microarray Readout System after PCR Amplification

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