Microfluidic device for trapping and monitoring three dimensional multicell spheroids using electrical impedance spectroscopy

Luongo, Kevin; Holton, Angela; Spence, Paige; Ng, Beng; Deschenes, Robert J.; Sundaram, S.; Bhansali, Shekhar

doi:10.1063/1.4809590

Cited by 29 publications

(27 citation statements)

References 35 publications

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“…1 Recent efforts have also been made into dielectric measurements of more complex biological tissue such as cancer spheroids in order to monitor drug effects and interactions. 2 Other biological processes have also been dielectrically monitored such as cell cluster formation and epithelial cell differentiation. 3 An important application of dielectric cell characterization and differentiation monitoring has been applied to human pancreatic islets to assess regeneration potential.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic platform for assessing pancreatic islet functionality through dielectric spectroscopy

et al. 2015

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Human pancreatic islets are seldom assessed for dynamic responses to external stimuli. Thus, the elucidation of human islet functionality would provide insights into the progression of diabetes mellitus, evaluation of preparations for clinical transplantation, as well as for the development of novel therapeutics. The objective of this study was to develop a microfluidic platform for in vitro islet culture, allowing the multi-parametric investigation of islet response to chemical and biochemical stimuli. This was accomplished through the fabrication and implementation of a microfluidic platform that allowed the perifusion of islet culture while integrating real-time monitoring using impedance spectroscopy, through microfabricated, interdigitated electrodes located along the microchamber arrays. Real-time impedance measurements provide important dielectric parameters, such as cell membrane capacitance and cytoplasmic conductivity, representing proliferation, differentiation, viability, and functionality. The perifusion of varying glucose concentrations and monitoring of the resulting impedance of pancreatic islets were performed as proof-of-concept validation of the lab-on-chip platform. This novel technique to elucidate the underlying mechanisms that dictate islet functionality is presented, providing new information regarding islet function that could improve the evaluation of islet preparations for transplantation. In addition, it will lead to a better understanding of fundamental diabetes-related islet dysfunction and the development of therapeutics through evaluation of potential drug effects. V C 2015 AIP Publishing LLC. [http://dx

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Section: Introductionmentioning

confidence: 99%

Microfluidic platform for assessing pancreatic islet functionality through dielectric spectroscopy

et al. 2015

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“…In addition, a microfluidic device with electrodes embedded into the top and bottom of the microfluidic channel was used for trapping and monitoring MCF-7 microtumor spheroids. 53 Spheroids induced changes in the magnitude of the solution resistance consistent with increases in spheroid diameter. This device is suitable for fabrication of multiple channels and electrodes for high-throughput analysis.…”

Section: Electrode Array Devicesmentioning

confidence: 91%

Micro/nanoelectrochemical probe and chip devices for evaluation of three-dimensional cultured cells

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Şen

Shiku

et al. 2017

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Herein, we present an overview of recent research progress in the development of micro/nanoelectrochemical probe and chip devices for the evaluation of three-dimensional (3D) cultured cells. First, we discuss probe devices: a general outline, evaluation of O 2 consumption, enzyme-modified electrodes, evaluation of endogenous enzyme activity, and the collection of cell components from cell aggregates are discussed. The next section is focused on integrated chip devices: a general outline, electrode array devices, smart electrode array devices, droplet detection of 3D cultured cells, cell manipulation using dielectrophoresis (DEP), and electrodeposited hydrogels used for fabrication of 3D cultured cells on chip devices are discussed. Finally, we provide a summary and discussion of future directions of research in this field.

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“…15 In the case of the planar electrodes, the target cell should be confined on the electrodes with the support of chemical interaction. 16 Otherwise, the electrical response of the cell might contain a large amount of the medium, which would be trivial information.…”

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confidence: 99%

Discrimination between the human prostate normal cell and cancer cell by using a novel electrical impedance spectroscopy controlling the cross-sectional area of a microfluidic channel

et al. 2013

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The prostate biopsy method shows a high false negative result because the suspicious tissue considered as cancer is not confirmed during tissue sampling. Thus, repeated biopsy procedures and diagnostic errors in relation to prostate cancer frequently occur. The purpose of this research is to enhance the prostate cancer detection rate by using microfluidic electrical impedance spectroscopy (lEIS), which allows real-time measurement of the electrical impedance of a single human prostate normal cell and cancer cell. The lEIS was equipped with a movable flexible membrane, which is operated by pneumatic pressure to capture the single cell on the surface of sensing electrodes. The forced tight contact between the cell and electrodes makes it possible to measure the electrical characteristics of the cell with a high sensitivity. The lEIS discriminates well between normal human prostate cells (RWPE-1) and cancer cells (PC-3) at 8.7 kHz based on the electrical signal responses of the cells. The average difference rates of admittance magnitude and susceptance are 54.55% and 54.59%, respectively. The developed lEIS also shows high repeatability, which was verified by a deionized water test conducted before and after each cell assay; the maximum variance of both the impedance and admittance at 8.7 kHz was as small as 9.48%. V C 2013 AIP Publishing LLC. [http://dx

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Microfluidic device for trapping and monitoring three dimensional multicell spheroids using electrical impedance spectroscopy

Cited by 29 publications

References 35 publications

Microfluidic platform for assessing pancreatic islet functionality through dielectric spectroscopy

Microfluidic platform for assessing pancreatic islet functionality through dielectric spectroscopy

Micro/nanoelectrochemical probe and chip devices for evaluation of three-dimensional cultured cells

Discrimination between the human prostate normal cell and cancer cell by using a novel electrical impedance spectroscopy controlling the cross-sectional area of a microfluidic channel

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