Microfluidic hydrodynamic focusing based synthesis of POPC liposomes for model biological systems

Abstract

Elsevier's AAM Policy: Authors retain the right to use the accepted author manuscript for personal use, internal institutional use and for permitted scholarly posting provided that these are not for purposes of commercial use or systematic distribution.Elsevier believes that individual authors should be able to distribute their AAMs for their personal voluntary needs and interests, e.g. posting to their websites or their institution's repository, e-mailing to colleagues. However, our policies differ regarding the systematic aggregation or distribution of AAMs to ensure the sustainability of the journals to which AAMs are submitted. Therefore, deposit in, or posting to, subjectoriented or centralized repositories (such as PubMed Central), or institutional repositories with systematic posting mandates is permitted only under specific agreements between Elsevier and the repository, agency or institution, and only consistent with the publisher's policies concerning such repositories. AbstractLipid vesicles have received significant attention in areas ranging from pharmaceutical and biomedical engineering to novel materials and nanotechnology. Microfluidic-based synthesis of liposomes offers a number of advantages over the more traditional synthesis methods such as extrusion and sonication. One such microfluidic approach is microfluidic hydrodynamic focusing (MHF), which has been used to synthesize nanoparticles and vesicles of various lipids. We show here that this method can be utilized in synthesis of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) vesicles with controllable size. Since POPC is among the primary constituents of cellular membranes, this work is of direct applicability to modelling of biological systems and development of nanocontainers with higher biologic compatibility for pharmaceutical and medical applications.

The Use of an Efficient Microfluidic Mixing System for Generating Stabilized Polymeric Nanoparticles for Controlled Drug Release

“…The diameters of liposomes obtained using different microfluidics devices were also decreased by changing the flow rate ratio. 40) In accordance with the above discussion regarding the relationship between PDI and flow rate ratio, an increase in PDI was observed by increasing the flow rate ratio (Fig. 5B), consistent with several previous articles 27,28) but the opposite of that reported in another study.…”

The Impact of Solvent Selection: Strategies to Guide the Manufacturing of Liposomes Using Microfluidics

“…Typically, IPA has been used as the lipid solvent in microfluidic processes [8,[22][23][24], with fewer studies exploring other solvents such as ethanol as a less toxic alternative for medicinal applications, which would also comply with routine industrial processes [25]. However, the choice of solvent used during microfluidics may have an impact; Zook and Vreeland hypothesised that liposomes formed The relationship between aqueous solubility and the number of carbon atoms in alcohols determined by surface tension (adapted from [19]) and physical properties of methanol, ethanol, and isopropanol.…”

Nanomedicine Scale-up Technologies: Feasibilities and Challenges

“…In order to get the desired size of nanoparticles, several cycles (may range from 50 to even 100) are required which is the limitation of this method. Microfluidic-based liposomes preparation is recently reported in the literature (12). This technology offers a number of advantages over the more traditional methods of preparation of liposomes such as extrusion and sonication.…”

“…This technology offers a number of advantages over the more traditional methods of preparation of liposomes such as extrusion and sonication. Microfluidic hydrodynamic focusing has been used to synthesize nanoparticles and vesicles of various lipids (12)(13)(14)(15).…”