Microfluidic immunosensor for rapid and sensitive detection of bovine viral diarrhea virus

Heinze, Brian C.; Song, Jae Young; Lee, Changhee; Najam, Anbar; Yoon, Jeong Yeol

doi:10.1016/j.snb.2009.02.058

Cited by 39 publications

(28 citation statements)

References 11 publications

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“…Over the last two decades, immune biosensors have become powerful and versatile detection tools for applications requiring on-site analysis, primarily because of their speed of analysis and simplicity of operation (20,41). Stratophase Ltd. (Romsey, UK) has developed a transportable FMD detection unit using its optical microchip technology, which measures changes in the refractive index (RI) at the surface of an optical chip (http://www.biooptocsworld.com/).…”

Section: Infrared Thermography As a Diagnostic Tool For An Early Detementioning

confidence: 99%

Recent progress in the diagnosis of foot-and-mouth disease: Rapid field-based assays

Niedbalski¹

2016

Medycyna Weterynaryjna

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The rapid and accurate diagnosis of foot-and-mouth disease (FMD) is critical for effective disease control. Most of laboratory-based methods can provide objective results within a few hours of sample receipt. However, the time taken to transport suspect material to the laboratory can be unacceptably long, often precluding laboratory confirmation in the event of an outbreak. Therefore, rapid and easy-to-perform tests, which can be used in the field (on-site) in case of a suspected disease outbreak, would be a valuable tool for veterinarians in the initial diagnosis of FMDV. The lateral flow immunochromatographic (LFI) test has been efficiently used for the detection of specific antibodies against FMDV non-structural proteins. FMDV serotypes O, A, Asia 1, SAT 2 and non-serotype-specific FMDV. Recently, several FMD-specific real-time RT-PCR (rRT-PCR) assays have been transferred to portable mobile platforms and evaluated for the detection of FMDV in the field: the Cepheid SmartCycler Real-time PCR machine, Enigma FL and LightCycler Nano System. The BioSeeq-Vet (Smiths Detection) was the first commercially available truly portable PCR instrument for the detection of viral RNA in the field. Additionally, a new portable amplification platform Genie I for the on-site detection of viral RNA by reverse-transcription loop-mediated isothermal amplification (RT-LAMP) has also been developed and evaluated. Infrared thermography (IRT), a quantitative method for the assessment of body surface temperature, may also be a useful tool for the early detection of FMDV in the field. In the absence of overt clinical signs, the IRT rapid screening test, which measures heat emission, can be essential in selecting potentially infected animals. Microarrays, a recently introduced technology for the laboratory diagnosis of FMD, offers greater screening capabilities for FMDV detection and can be regarded as an alternative to classical diagnostic methods.

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Section: Infrared Thermography As a Diagnostic Tool For An Early Detementioning

confidence: 99%

Recent progress in the diagnosis of foot-and-mouth disease: Rapid field-based assays

Niedbalski¹

2016

Medycyna Weterynaryjna

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“…In the microfluidic chip, the virus was detected in low concentration, down to a concentration of 103 TCID50 mL −1 . 187 Vesicular stomatitis rhabdovirus was used as a generic model for capture, detection, and identification of a number of pathogenic viruses by field modulated light scattering. 188 For NA detection, Lu et al 67 showed a gold nanorodsbased biosensor for the detection of HBV DNA based on fluorescence resonance energy transfer.…”

Section: Optical Light Scatteringmentioning

confidence: 99%

Nanoscale virus biosensors: state of the art

Krejčová¹,

Michálek²,

Rodrigo³

et al. 2015

NDD

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Abstract:Since the beginning of the new millennium, viruses have shown huge epidemiological and pandemic potential: severe acute respiratory syndrome (SARS) in 2002, pandemic swine flu in 2009, and last but not least the West African Ebola outbreak in 2014. The occurrence and spread of the new virus in pandemic dimension poses a threat to the health and lives of 7 billion people worldwide. There is a growing urgency for highly sensitive and selective detection techniques, usable for a wide number of applications, including disease diagnosis, pharmaceutical research, agriculture, as well as preventive measures. Nanobiosensors represent a new promising tool for virus detection. This review gives a brief survey of the issue of viral detection, comprising diagnostics of target structure of viruses such as nucleic acids or proteins. This review covers different detection principles, methods of fabrication, and applications of virus biosensors.

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“…Fluorescence optical systems are preferred over light absorbance ones due to their higher sensitivity on small platforms [156]. There are also reports of small immunosensors using SPR [157,158], chemiluminescence [159], light scattering [160] and electrochemical systems [161].…”

Section: Miniaturisation Of Immunosensorsmentioning

confidence: 99%

Trends and Perspectives in Immunosensors

2012

Antibodies Applications and New Developments

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Immunosensors are devices that comprise both a biomolecular recognition system, such as an antibody and its corresponding antigen, and a transducer to translate the high affinity and specific binding event into a physical signal.Antibodies are produced by an immunological response to the presence of a foreign substance called an antigen. Antibodies may be immobilised onto a variety of platforms including bulk planar surfaces and nanoparticles by either covalent or adsorption strategies. Different interfaces between the bio-components and the detector are available to monitor in 'real-time' the signal generated by biological interactions. The transducers detect, for example, the change in electron transfer, absorbance, fluorescence, refractive index, mass change or heat transfer as the antibody binds to the antigen of interest. Such analytical devices have allowed a wide range of analytes to be identified and quantified such as pathogens, toxins, environmental food contaminants and disease biomarkers.The demand for sensitive, rapid, 'on-site' techniques has taken advantage of the latest advances in microfluidics and nanotechnology. This chapter will highlight current trends in immobilisation, micro/nano-fluidics/ and transducers utilised. A number of examples outlining the exploitation of these elements in immunosensors and their successful applications will be described.

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Microfluidic immunosensor for rapid and sensitive detection of bovine viral diarrhea virus

Cited by 39 publications

References 11 publications

Recent progress in the diagnosis of foot-and-mouth disease: Rapid field-based assays

Recent progress in the diagnosis of foot-and-mouth disease: Rapid field-based assays

Nanoscale virus biosensors: state of the art

Trends and Perspectives in Immunosensors

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