Microfluidic Platform for Enzyme-Linked and Magnetic Particle-Based Immunoassay

Bhalla, Nikhil; Chung, Danny; Chang, Yaw Jen; Uy, Kimberly Jane; Ye, Yi Ying; Chin, Ting‐Yu; Yang, Hao; Pijanowska, Dorota G.

doi:10.3390/mi4020257

Cited by 20 publications

(13 citation statements)

References 21 publications

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“…The proposed design is comparable in complexity to other microfluidic test platforms such as the one proposed by Bhalla et al [ 30 ]. Their work demonstrated a two-step validation method for Adiponectin detection using ELISA.…”

Section: Resultsmentioning

confidence: 92%

Monitoring Aquaculture Water Quality: Design of an Early Warning Sensor with Aliivibrio fischeri and Predictive Models

Silva

Yang

Pires

et al. 2018

Sensors

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A novel toxicity-warning sensor for water quality monitoring in recirculating aquaculture systems (RAS) is presented. The design of the sensor system mainly comprises a whole-cell biosensor. Aliivibrio fischeri, a luminescent bacterium widely used in toxicity analysis, was tested for a mixture of known fish-health stressors, namely nitrite, un-ionized ammonia, copper, aluminum and zinc. Two toxicity predictive models were constructed. Correlation, root mean squared error, relative error and toxic behavior were analyzed. The linear concentration addition (LCA) model was found suitable to ally with a machine learning algorithm for prediction of toxic events, thanks to additive behavior near the limit concentrations for these stressors, with a root-mean-squared error (RMSE) of 0.0623, and a mean absolute error of 4%. The model was proved to have a smaller relative deviation than other methods described in the literature. Moreover, the design of a novel microfluidic chip for toxicity testing is also proposed, which is to be integrated in a fluidic system that functions as a bypass of the RAS tank to enable near-real time monitoring. This chip was tested with simulated samples of RAS water spiked with zinc, with an EC50 of 6,46E-7 M. Future work will be extended to the analysis of other stressors with the novel chip.

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Section: Resultsmentioning

confidence: 92%

Monitoring Aquaculture Water Quality: Design of an Early Warning Sensor with Aliivibrio fischeri and Predictive Models

Silva

Yang

Pires

et al. 2018

Sensors

View full text Add to dashboard Cite

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“…The method is based on enzyme‐linked immunoassay, where the antibody is immobilized on nitrocellulose and magnetic beads to label the analyte. The chip takes 2 h 15 min to detect protein followed by fluorescence measurement [159]. Micromachined MNPs were also used as separators in microfluidic systems for magnetic particle‐based bioseparation.…”

Section: Applicationsmentioning

confidence: 99%

Magnetic nanoparticles in microfluidic and sensing: From transport to detection

et al. 2020

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Superparamagnetic nanoparticles are attracting significant attention. Therefore, being explored in microsystems for a wide range of applications. Typical examples include labon-a-chip and microfluidics for synthesis, detection, separation, and transportation of different bioanalytes, such as biomolecules, cells, and viruses to develop portable, sensitive, and cost-effective biosensing systems. Particularly, microfluidic systems incorporated with magnetic nanoparticles and, in combination with magnetoresistive sensors, shift diagnostic and analytical methods to a microscale level. In this context, nanotechnology enables the miniaturization and integration of a variety of analytical functions in a single chip for manipulation, detection, and recognition of bioanalytes reliably and flexibly. In consideration of the above, recent development and benefits are elaborated herein to discuss the role of magnetic nanoparticles inside the microchannels to design highly efficient disposable point-of-care applications from transportation to the detection of bioanalytes.

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“…The development of magnetic particles functionalized with antibodies have been leveraged into automated detection platforms (Bhalla et al 2013; Lien et al 2011; Yun et al 2007). Through automation, standard tests can be rendered hands-free, and using microfluidic platforms less sample or reagents are consumed.…”

Section: Development Of Potential Alternative and Future Biosensors Umentioning

confidence: 99%

Strategies in Ebola virus disease (EVD) diagnostics at the point of care

Coarsey

Esiobu

Narayanan

et al. 2017

Critical Reviews in Microbiology

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Ebola virus disease (EVD) is a devastating, highly infectious illness with a high mortality rate. The disease is endemic to regions of Central and West Africa, where there is limited laboratory infrastructure and trained staff. The recent 2014 West African EVD outbreak has been unprecedented in case numbers and fatalities, and has proven that such regional outbreaks can become a potential threat to global public health, as it became the source for the subsequent transmission events in Spain and the USA. The urgent need for rapid and affordable means of detecting Ebola is crucial to control the spread of EVD and prevent devastating fatalities. Current diagnostic techniques include molecular diagnostics and other serological and antigen detection assays; which can be time-consuming, laboratory-based, often require trained personnel and specialized equipment. In this review, we discuss the various Ebola detection techniques currently in use, and highlight the potential future directions pertinent to the development and adoption of novel point-of-care diagnostic tools. Finally, a case is made for the need to develop novel microfluidic technologies and versatile rapid detection platforms for early detection of EVD.

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Microfluidic Platform for Enzyme-Linked and Magnetic Particle-Based Immunoassay

Cited by 20 publications

References 21 publications

Monitoring Aquaculture Water Quality: Design of an Early Warning Sensor with Aliivibrio fischeri and Predictive Models

Monitoring Aquaculture Water Quality: Design of an Early Warning Sensor with Aliivibrio fischeri and Predictive Models

Magnetic nanoparticles in microfluidic and sensing: From transport to detection

Strategies in Ebola virus disease (EVD) diagnostics at the point of care

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