Microfluidic retention of progressively motile zebrafish sperms

Panigrahi, Bivas; Chen, Chia Yuan

doi:10.1039/c9lc00534j

Cited by 18 publications

(8 citation statements)

References 36 publications

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“…As the final step, the PDMS channel was detached from the mold, and the inlet & outlet were punched through a biopsy puncher. The microchannel was subsequently bonded with another PDMS layer through an oxygen plasma process 30 – 32 . The principle was to make the microchannel and PDMS bond together to avoid fluid leakage 33 .…”

Section: Methodsmentioning

confidence: 99%

The evaluation of zebrafish cardiovascular and behavioral functions through microfluidics

Subendran

Wang

et al. 2021

Sci Rep

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This study proposed a new experimental approach for the vascular and phenotype evaluation of the non-anesthetized zebrafish with representative imaging orientations for heart, pectoral fin beating, and vasculature views by means of the designed microfluidic device through inducing the optomotor response and hydrodynamic pressure control. In order to provide the visual cues for better positioning of zebrafish, computer-animated moving grids were generated by an in-house control interface which was powered by the larval optomotor response, in conjunction with the pressure suction control. The presented platform provided a comprehensive evaluation of internal circulation and the linked external behaviors of zebrafish in response to the cardiovascular parameter changes. The insights from these imaging sections was extended to identify the linkage between the cardiac parameters and behavioral endpoints. In addition, selected chemicals such as ethanol and caffeine were employed for the treatment of zebrafish. The obtained findings can be applicable for future investigation in behavioral drug screening serving as the forefront in psychopharmacological and cognition research.

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Section: Methodsmentioning

confidence: 99%

The evaluation of zebrafish cardiovascular and behavioral functions through microfluidics

Subendran

Wang

et al. 2021

Sci Rep

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“…As illustrated in Figure 1B, geometrical micro-confinements inside the microfluidic channels of a baffle-based system produced flow-induced shear forces. The generated velocity shear gradients caused lateral migration of the activated zebrafish sperms toward the stagnation for progressive motile sperm retrieval [64]. Using this technique, a 44% improvement in sperm-retrieval efficiency was reported.…”

Section: Geometry and Chip Designmentioning

confidence: 99%

“…(A) Micro-pillar arrays imitate the filter-like characteristics of the female reproductive tract[62]. (B) Geometrical micro-confinement in the side channels of the microfluidic device guides the activated sperms toward the stagnation zone[64]. (C) The stricture inside the microfluidic channel mimics the variable width of the junctions within the female reproductive tract[65].…”

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confidence: 99%

Recent Microfluidic Innovations for Sperm Sorting

et al. 2021

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Sperm selection is a clinical need for guided fertilization in men with low-quality semen. In this regard, microfluidics can provide an enabling platform for the precise manipulation and separation of high-quality sperm cells through applying various stimuli, including chemical agents, mechanical forces, and thermal gradients. In addition, microfluidic platforms can help to guide sperms and oocytes for controlled in vitro fertilization or sperm sorting using both passive and active methods. Herein, we present a detailed review of the use of various microfluidic methods for sorting and categorizing sperms for different applications. The advantages and disadvantages of each method are further discussed and future perspectives in the field are given.

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“…Mammalian species only represent a small portion of endangered species, but they are the most studied species regarding ARTs. Nevertheless, dedicated microfluidic systems have been developed for non-mammalian vertebrates, such as for zebrafish sperm analysis and activation [66,67], and embryo culture [68], notably to generate more fundamental knowledge about embryogenesis, neurophysiology [69], and for toxicity screening [70].…”

Section: Next Steps To Better Understand and Assist Wildlife Reproduction Using Microfluidicsmentioning

confidence: 99%

Understanding and Assisting Reproduction in Wildlife Species Using Microfluidics

Gac

Ferraz

Venzac

et al. 2021

Trends in Biotechnology

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Microfluidic retention of progressively motile zebrafish sperms

Abstract: A microfluidic concept was demonstrated for the orientation control of progressively motile zebrafish sperms through the hydrodynamic manipulation.

Cited by 18 publications

References 36 publications

The evaluation of zebrafish cardiovascular and behavioral functions through microfluidics

The evaluation of zebrafish cardiovascular and behavioral functions through microfluidics

Recent Microfluidic Innovations for Sperm Sorting

Understanding and Assisting Reproduction in Wildlife Species Using Microfluidics

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