Microfluidic systems as a novel approach for microalgal bioprocess

Karacaoglu, Beyza; İnan, Benan; Özçimen, Didem

doi:10.1016/j.bej.2023.108959

Cited by 7 publications

(1 citation statement)

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“…Due to the broadness of this research field and the large diversity and complexity of microorganisms, different aspects of how microfluidic technologies can be used for the analysis of single microorganisms have been previously reviewed. [ 16,215–221 ] In this section, we provide representative recent examples of integrated microfluidic devices designed for the isolation and on‐chip analysis of single microorganisms. Similar to many examples discussed in the previous sections, single microorganisms are often trapped in microtraps/wells/chambers and are subjected to a stimulus, then analyzed via chosen means.…”

Section: Recent Advances In Integrated Single‐cell Separation and On‐...mentioning

confidence: 99%

Integrated Microfluidics for Single‐Cell Separation and On‐Chip Analysis: Novel Applications and Recent Advances

Kutluk,

Viefhues,

Constantinou

2024

Small Science

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From deciphering infection and disease mechanisms to identifying novel biomarkers and personalizing treatments, the characteristics of individual cells can provide significant insights into a variety of biological processes and facilitate decision‐making in biomedical environments. Conventional single‐cell analysis methods are limited in terms of cost, contamination risks, sample volumes, analysis times, throughput, sensitivity, and selectivity. Although microfluidic approaches have been suggested as a low‐cost, information‐rich, and high‐throughput alternative to conventional single‐cell isolation and analysis methods, limitations such as necessary off‐chip sample pre‐ and post‐processing as well as systems designed for individual workflows have restricted their applications. In this review, a comprehensive overview of recent advances in integrated microfluidics for single‐cell isolation and on‐chip analysis in three prominent application domains are provided: investigation of somatic cells (particularly cancer and immune cells), stem cells, and microorganisms. Also, the use of conventional cell separation methods (e.g., dielectrophoresis) in unconventional or novel ways, which can advance the integration of multiple workflows in microfluidic systems, is discussed. Finally, a critical discussion related to current limitations of integrated microfluidic single‐cell workflows and how they could be overcome is provided.

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Section: Recent Advances In Integrated Single‐cell Separation and On‐...mentioning

confidence: 99%