Microfluidic systems for rapid antibiotic susceptibility tests (ASTs) at the single-cell level

Abstract

<p>Recent developments of microfluidics-based antibiotic susceptibility tests (ASTs) at the single-cell or single-molecule level are summarized for guiding antibiotic treatment.</p>

An In Vivo Microfluidic Study of Bacterial Load Dynamics and Absorption in the C. elegans Intestine

“…Implementing microfluidic approaches for advanced and accurate in vivo imaging of bacterial colony dynamics and digestion kinetics opens the path to enhance the information content, throughput, and versatility of assays targeting C. elegans microbiota–gut interaction. For instance, microfluidic platforms would be especially suitable for parallel on-chip assays based on worm feeding with different bacterial strains and/or the application of different antimicrobial compounds [ 62 ]. Likewise, a range of C. elegans mutants, presenting specific drug-dependent microbial–host interaction features, could be observed simultaneously.…”

One cell at a time: droplet-based microbial cultivation, screening and sequencing

“…Microfluidic-based methods are a promising tool 2016) American Society for Microbiology). f MSP isolated microorganisms from deep-sea sediments samples ◂ for rapid ASTs because of their miniaturization and automation (Zhang et al 2020). Various microfluidics-based methods have been developed for ASTs, such as microchamber, microchannel, and droplet-based tools (Hassan and Zhang 2020).…”

Botulinum toxin as an ultrasensitive reporter for bacterial and SARS-CoV-2 nucleic acid diagnostics

“…However, traditional culture-based methods are usually time-consuming and tedious ( Forne et al, 2000 ; Guan et al, 2013 ). Currently, novel biosensors based diagnostic strategies have been developed with an improved sensitivity and accuracy ( Ren et al, 2019 ; Song et al, 2019 ; Yoo and Lee, 2016 ; Zhang et al, 2020 ). Those sensors include ( Hong et al, 2020 ; Wan et al, 2018 ; Wang and Alocilja, 2015 ; Wu et al, 2015 ) enzyme-based reporters [horseradish peroxidase (HRP) ( Wen et al, 2016 ), alkaline phosphatase (ALP) ( Han et al, 2020 ), and catalase ( de la Rica and Stevens, 2012 )], fluorescent molecule-based reporters [Fluorescein probes ( Cardoso et al, 2011 ) and Molecular Beacons ( Cao et al, 2014 )] and nanomaterial-based reporters [magneto-DNA nanoparticle system ( Chung et al, 2013 ), Gold nanoparticles ( Yang et al, 2020 )].…”