Microfluidics for Sperm Selection

Smith, Gary D.; Rocha, A.M.; Keller, Laura

doi:10.1007/978-1-4939-1411-1_5

Cited by 4 publications

(1 citation statement)

References 47 publications

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“…These selected spermatozoa show important favorable changes such as: proper nuclear compaction, exchange of nuclear proteins and CD 52 glycopeptide buildup, rich in sialic acid residues; this last characteristic of the sperm maturation process is the main one responsible for the negative value charge in the sperm plasmatic membrane. Concurrently, this loading value enables a better in vivo interaction with the oocyte membrane and avoids non-specific aggregations in the female tract, as well as among the spermatozoa themselves ( Ishijima et al ., 1991 ; Smith et al ., 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Impact of the Z potential technique on reducing the sperm DNA fragmentation index, fertilization rate and embryo development

Duarte¹,

Nunez²,

Wong³

et al. 2017

JBRA Assisted Reproduction

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BackgroundIn assisted reproduction procedures, we need to develop and enhance new protocols to optimize sperm selection. The aim of this study is to evaluate the ability of the Z potential technique to select sperm with intact DNA in non-normospermic patients and evaluate the impact of this selection on embryonic development.MethodsWe analyzed a total of 174 human seminal samples with at least one altered parameter. We measured basal, post density gradients, and post density gradients + Z potential DNA fragmentation index. To evaluate the impact of this technique on embryo development, 54 cases were selected. The embryo development parameters evaluated were fertilization rate, cleavage rate, top quality embryos at the third day and blastocysts rate.ResultsWe found significant differences in the study groups when we compared the sperm fragmentation index by adding the Z potential technique to density gradient selection vs. density gradients alone. Furthermore, there was no significant difference in the embryo development parameters between the low sperm fragmentation index group vs. the moderate and high sperm fragmentation index groups, when selecting sperms with this new technique.ConclusionsThe Z potential technique is a very useful tool for sperm selection; it significantly reduces the DNA fragmentation index and improves the parameters of embryo development. This technique could be considered routine for its simplicity and low cost.

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Section: Introductionmentioning

confidence: 99%

Impact of the Z potential technique on reducing the sperm DNA fragmentation index, fertilization rate and embryo development

Duarte¹,

Nunez²,

Wong³

et al. 2017

JBRA Assisted Reproduction

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Advances in Microfluidics‐Based Assisted Reproductive Technology: From Sperm Sorter to Reproductive System‐on‐a‐Chip

2018

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The fields of assisted reproductive technology (ART) and in vitro fertilization (IVF) have progressed rapidly, yet still need further improvements. Microfluidic technology can incorporate various ART procedures such as embryo/gamete (sperm/oocyte) analysis, sorting, manipulation, culture, and monitoring. The introduction of paper‐based and droplet‐based microfluidics further improves the commercialization potential of this technology. The progress in 3D printing technology allows for the integration of microfluidics with tissue engineering that may revolutionize current practices in biology and medicine. This review categorizes ART methods according to continuous‐flow microfluidics, paper‐based microfluidics, droplet‐based microfluidics, and organ‐on‐a‐chip. The advances are summarized and potential opportunities in infertility diagnosis, sperm selection, sperm guidance, oocyte selection, insemination, embryo culture, embryo monitoring, and cryopreservation are identified. While some advances of continuous‐flow microfluidics for ART have already been reviewed, other microfluidic techniques are still in their early stages. It is envisioned that advances in droplet‐based microfluidics, especially digital microfluidics, will allow for more progress in human IVF, particularly single embryo transfer. Droplet‐based microfluidics may also lead to fully integrated and high‐throughput platforms for animal IVF. Recent advances in organ‐on‐a‐chip including ovary/uterus/oviduct‐on‐chip platforms hold promise for the integration of the whole human reproductive system‐on‐a‐chip for clinical applications.

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Recent Advances and Challenges in the Development of Novel Sperm Sexing Methods

Yata

2022

Sperm Sexing and Its Role in Livestock Production

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Microfluidics for Sperm Selection

Cited by 4 publications

References 47 publications

Impact of the Z potential technique on reducing the sperm DNA fragmentation index, fertilization rate and embryo development

Impact of the Z potential technique on reducing the sperm DNA fragmentation index, fertilization rate and embryo development

Advances in Microfluidics‐Based Assisted Reproductive Technology: From Sperm Sorter to Reproductive System‐on‐a‐Chip

Recent Advances and Challenges in the Development of Novel Sperm Sexing Methods

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