Microhardness and SEM after CO<sub>2</sub> laser irradiation or fluoride treatment in human and bovine enamel

Souza‐Gabriel, Aline Evangelista; Colucci, Vivian; Turssi, Cecília Pedroso; Serra, Mônica Campos; Corona, Silmara Aparecida Milori

doi:10.1002/jemt.20827

Cited by 44 publications

(40 citation statements)

References 34 publications

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“…Two previous pH-cycling studies [19,20] utilizing net de-rather than remineralizing models, did not highlight any differences between BE and HE in terms of their response to fluoride. This, together with other [2] and especially the present observations perhaps highlights that while there are structural and chemical differences between BE and HE, these are easily overshadowed by the impact of differences in mineral distributions and extent of demineralization.…”

Section: Discussionmentioning

confidence: 74%

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Fluoride dose-response of human and bovine enamel artificial caries lesions under pH-cycling conditions

Lippert

Juthani

2015

Clin Oral Invest

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Objectives This laboratory study aimed to: a) compare the fluoride dose-response of different caries lesions created in human and bovine enamel (HE/BE) under pH-cycling conditions, and b) investigate the suitability of Knoop and Vickers surface microhardness (K-SMH/V-SMH) in comparison to transverse microradiography (TMR) to investigate lesion de-and remineralization.Materials and Methods Caries lesions were formed using three different protocols (Carbopol, hydroxyethylcellulose-HEC, methylcellulose-MeC) and assigned to 24 groups using V-SMH, based on a 2 (enamel types)×3 (lesion types)×4 (fluoride concentrations used during pH-cycling-simulating 0/250/1100/2800 ppm F as sodium fluoride dentifrices) factorial design. Changes in mineral content and structural integrity of lesions were determined before and after pH-cycling. Data were analyzed using three-way ANOVA.Results BE was more prone to demineralization than HE. Both enamel types showed similar responses to fluoride with BE showing more remineralization (as change in integrated mineral loss and lesion depth reduction), although differences between tissues were already present at lesion baseline. Carbopol and MeC lesions responded well to fluoride, whereas HEC lesions were almost inert. K-and V-SMH correlated well with each other and with the integrated mineral loss data, although better correlations were found for HE than for BE, and for MeC than for Carbopol lesions. Hardness data for HEC lesions correlated only with surface zone mineral density data.Conclusion BE is a suitable surrogate for HE under pH-cycling conditions. Clinical Relevance The in vitro modelling of dental caries is complex and requires knowledge of lesion behavior, analytical techniques and employed hard tissues.

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Section: Discussionmentioning

confidence: 74%

“…A recent study [18] highlighted that BE may be more prone to remineralization than HE, although the study was somewhat limited in scope. No differences between tissues were found in their fluoride response under net demineralizing conditions in two other pH-cycling studies [19,20], highlighting the need for a more comprehensive study.…”

Section: Introductionmentioning

confidence: 92%

Fluoride dose-response of human and bovine enamel artificial caries lesions under pH-cycling conditions

Lippert

Juthani

2015

Clin Oral Invest

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“…Therefore, 68 papers were included in the final review. The studies covered seven main categories: morphology (5 studies) (22)(23)(24)(25)(26), chemical composition (11 studies) (27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37), physical properties (12 studies) (38)(39)(40)(41)(42)(43)(44)(45)(46)(47)(48)(49), dental caries (7 studies) (6,9,(50)(51)(52)(53)(54), dental erosion/abrasion (10 studies) (55)(56)(57)(58)(59)(60)(61)(62)(63)(64), bonding/adhesive strength (17 studies) (65)(66)(67)(68)…”

Section: Search Resultsmentioning

confidence: 99%

“…Dental caries studies Table 1 shows the details of the seven studies reviewed (6,9,(50)(51)(52)(53)(54). There were four in vitro studies (6,9,50,51), one in situ study (52), and two in vitro/in situ studies (53,54).…”

Section: In Vitro Permanent Enamel Crown Nanoindentation Profilometrymentioning

confidence: 99%

“…There were four in vitro studies (6,9,50,51), one in situ study (52), and two in vitro/in situ studies (53,54). Only one study in this category compared dentin caries between human and bovine teeth (52), whereas the remaining studies compared only enamel caries between human and bovine teeth (6,9,(50)(51)(52)(53)(54).…”

Section: In Vitro Permanent Enamel Crown Nanoindentation Profilometrymentioning

confidence: 99%

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Bovine teeth as substitute for human teeth in dental research: a review of literature

2011

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The aim of this paper was to review in vitro and in situ studies that directly compared the use of bovine teeth as a substitute for human teeth in dental experiments. A PubMed search was conducted for papers published from 1953 to December 30, 2010 using the following keywords: "human bovine enamel" or "human bovine dentin" or "human bovine teeth". The abstracts of the studies resulting from the keyword search were read, and all papers that compared human and bovine teeth were fully read. Only original articles written in English and directly comparing human and bovine substrates were included in the review. The search was supplemented by manual searches of the reference lists from each identified paper. Out of 76 studies initially selected, 68 fulfilled the selection criteria for inclusion. The studies covered seven categories: dental morphology, chemical composition, physical properties, dental caries, dental erosion/abrasion, bonding/adhesive strength, and marginal microleakage. Inconsistent data exist regarding whether bovine teeth can be considered an appropriate substitute for human teeth in dental research. Morphological, chemical composition and physical property differences between the two substrates must be considered when interpreting results obtained from any experiment using bovine tooth substrate. (J Oral Sci 53, 273-282, 2011)

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The Enamel Microstructures of Bovine Mandibular Incisors

Wang

et al. 2012

The Anatomical Record

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Bovine teeth have been considered as an excellent substitute for human teeth for dental research, however, the enamel microstructures of bovine incisors that include arrangements of prisms and interprisms, and their spatial relationships have not been well described. The aim of this study was to investigate the detail enamel microstructures of bovine incisors. Eight bovine mandibular incisors were cut into 77 pieces at eight equal intervals either in the longitudinal direction or in the horizontal direction before each piece had been tangentially cut (parallel to enameldentin junction) through the middle of the enamel thickness. All the sectioned surfaces were treated 1 M HCl for 10 sec to expose the prisms and interprisms before observation by scanning electron microscopy. The parallel enamel prisms were located in all the outer enamel, the cervical region and the incisal ridge of the bovine incisors. Most labial inner enamel and the cingulum of lingual inner enamel were composed of the Hunter-Schreger bands with the characteristics of decussating groups of prisms and decussating planes between interprisms and prisms. The interprisms were thicker in the inner enamel than in the outer enamel.

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Microhardness and SEM after CO₂ laser irradiation or fluoride treatment in human and bovine enamel

Abstract: CO(2) laser may control caries progression more efficiently than fluoride sources and bovine teeth may be a suitable substitute for human teeth in studies of this nature.

Cited by 44 publications

References 34 publications

Fluoride dose-response of human and bovine enamel artificial caries lesions under pH-cycling conditions

Fluoride dose-response of human and bovine enamel artificial caries lesions under pH-cycling conditions

Bovine teeth as substitute for human teeth in dental research: a review of literature

The Enamel Microstructures of Bovine Mandibular Incisors

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Microhardness and SEM after CO2 laser irradiation or fluoride treatment in human and bovine enamel

Abstract: CO(2) laser may control caries progression more efficiently than fluoride sources and bovine teeth may be a suitable substitute for human teeth in studies of this nature.

Cited by 44 publications

References 34 publications

Fluoride dose-response of human and bovine enamel artificial caries lesions under pH-cycling conditions

Fluoride dose-response of human and bovine enamel artificial caries lesions under pH-cycling conditions

Bovine teeth as substitute for human teeth in dental research: a review of literature

The Enamel Microstructures of Bovine Mandibular Incisors

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Microhardness and SEM after CO₂ laser irradiation or fluoride treatment in human and bovine enamel