Micromagnet arrays enable precise manipulation of individual biological analyte–superparamagnetic bead complexes for separation and sensing

Rampini, Stefano; Li, P.; G, Lee

doi:10.1039/c6lc00707d

Cited by 40 publications

(40 citation statements)

References 125 publications

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“…Nanosized superparamagnetic beads functionalized with specific surface are versatile materials used for various applications in chemical assay, biosensors, bioseparation systems, and biomedical studies (Rampini et al 2015;Rampini et al 2016;Ran et al 2014). To provide specificity, magnet beads can be decorated with ligands, such as antibodies, aptamers, and peptides, qualifying them as useful tools for the purification, detection, and separation of biological analytes from complex samples (Muzard et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Single-domain antibodies as promising experimental tools in imaging and isolation of porcine epidemic diarrhea virus

Yang

Yin

et al. 2018

Appl Microbiol Biotechnol

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Single-domain antibody (sdAb) or nanobody possesses specific features non-accessible for conventional antibodies that make them suitable for research and biotechnological applications. Porcine epidemic diarrhea virus (PEDV) causes lethal diarrhea in piglets, resulting in great economic losses all over the world. To detect and isolate PEDV rapidly and accurately is important for the control and further research of the clinical PEDV strains. In this study, four sdAb fragments (sdAb-Mc19/29/30/37) targeting the membrane (M) protein of PEDV were selected from sdAb library that was constructed through M protein-immunized Camelus bactrianus. The selected sdAb-Mcs were solubly expressed in Escherichia coli. The functional characteristics analysis revealed that the recombinant sdAb-Mcs have excellent binding activity and specificity to M protein but have no neutralizing activity to PEDV. For further application, sdAb-Mc37 was conjugated with quantum dots to synthesize a nanoprobe for imaging PEDV in vero cells. The observed fluorescence in vero cells clearly reflects that PEDV virions can be reliably recognized and labeled by the nanoprobe. Furthermore, the sdAb-Mc29 was conjugated with superparamagnetic nanobeads to construct immunomagnetic nanobeads (IMNBs) used to isolate PEDV. One PEDV strain was successfully isolated from clinical fecal sample, suggesting IMNBs as a novel and efficient tool suitable for PEDV isolation from clinical samples. This study provided a novel application and substantiated the suitability of sdAb as a specific binder for the isolation of viruses.

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Section: Introductionmentioning

confidence: 99%

Single-domain antibodies as promising experimental tools in imaging and isolation of porcine epidemic diarrhea virus

Yang

Yin

et al. 2018

Appl Microbiol Biotechnol

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“…Novel magnetophoretic technologies aiming towards bio-medical applications have tremendously advanced for biomolecule separation, gene delivery and transfection, disease diagnosis and therapy on an individual cell level [184,185]. In particular, the versatility of magnetic shuttle technology has great potential in the logical manipulation of a specific cell selection, capture, transport and encapsulation with the support of superparamagnetic iron oxide nanoparticle (SPION) carriers within the magnetophoretic platform [186,187], as depicted in figure 24.…”

Section: Department Of Emerging Materials Science Dgistmentioning

confidence: 99%

“…Magnetic carriers are utilized via the endocytosis of SPIONs in the cells, or the biochemical binding of nanoparticle embedded beads with cell surface proteins [184,185]. With ~100 nm thick patterns, the governing force is more or less 10 pN, with the force equation, F = µ0(χvV) 2 ∇ H 2 .…”

Section: Department Of Emerging Materials Science Dgistmentioning

confidence: 99%

The 2017 Magnetism Roadmap

Sander

Valenzuela

Makarov

et al. 2017

J. Phys. D: Appl. Phys.

326

207

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Building upon the success and relevance of the 2014 Magnetism Roadmap, this 2017 Magnetism Roadmap edition follows a similar general layout, even if its focus is naturally shifted, and a different group of experts and, thus, viewpoints are being collected and presented. More importantly, key developments have changed the research landscape in very relevant ways, so that a novel view onto some of the most crucial developments is warranted, and thus, this 2017 Magnetism Roadmap article is a timely endeavour. The change in landscape is hereby not exclusively scientific, but also reflects the magnetism related industrial application portfolio. Specifically, Hard Disk Drive technology, which still dominates digital storage and will continue to do so for many years, if not decades, has now limited its footprint in the scientific Topical ReviewOriginal content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI. and research community, whereas significantly growing interest in magnetism and magnetic materials in relation to energy applications is noticeable, and other technological fields are emerging as well. Also, more and more work is occurring in which complex topologies of magnetically ordered states are being explored, hereby aiming at a technological utilization of the very theoretical concepts that were recognised by the 2016 Nobel Prize in Physics.Given this somewhat shifted scenario, it seemed appropriate to select topics for this Roadmap article that represent the three core pillars of magnetism, namely magnetic materials, magnetic phenomena and associated characterization techniques, as well as applications of magnetism. While many of the contributions in this Roadmap have clearly overlapping relevance in all three fields, their relative focus is mostly associated to one of the three pillars. In this way, the interconnecting roles of having suitable magnetic materials, understanding (and being able to characterize) the underlying physics of their behaviour and utilizing them for applications and devices is well illustrated, thus giving an accurate snapshot of the world of magnetism in 2017.The article consists of 14 sections, each written by an expert in the field and addressing a specific subject on two pages. Evidently, the depth at which each contribution can describe the subject matter is limited and a full review of their statuses, advances, challenges and perspectives cannot be fully accomplished. Also, magnetism, as a vibrant research field, is too diverse, so that a number of areas will not be adequately represented here, leaving space for further Roadmap editions in the future. However, this 2017 Magnetism Roadmap article can provide a frame that will enable the reader to judge where each subject and magnetism research field stands overall today and which directions it might take in the foreseeable future.The first mater...

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“…These processes still require several manufacturing steps, and face challenges related to the heterogeneous integration of magnetic materials with polymers, mainly PolyDiMethylSiloxane (PDMS), such as tedious alignment procedures (for locating the traps in the channels,) and tightness issues. Micromagnet arrays were reported to work as magnetic tweezers that enable precise manipulation of magnetic beads or cells (Henighan et al 2010;Rampini et al 2016), to traps rare cells (Chen et al 2014) or for single cell analysis (Jaiswal et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Arrays of high aspect ratio magnetic microstructures for large trapping throughput in lab-on-chip systems

Mekkaoui

Roy

Audry

et al. 2018

Microfluid Nanofluid

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Here we report a novel technology that requires simple fabrication process, to obtain highly efficient magnetic micro-traps. Developed micro-traps consist in chains of iron particles diluted in PolyDiMethylSiloxane (PDMS). X-ray tomography was used to analyze the microstructure of particle ordering in the PDMS membrane and revealed the predominance of chain-like agglomerates. Largest formed traps, with diameter ranging from 4 to 11 µm are found to be the most efficient. The self-organized trap arrays are characterized by a density of 1300 magnetic microtraps/mm 2 , with an average nearest neighbour distance, center-to-center, of 21 µm. Implemented in a microfluidic channel operating at a flow rate of 3 mL/h-a fluid flow of 8,3 mm/s-we measured trapping throughputs up to 7100 beads/min with an average distribution of 750 beads/mm 2. At fluid velocity up to 9,7 mm/s a trapping efficiency of 99,99% were measured. This novel technology allows to trap thousands of beads with throughputs that permit to compete with hydrodynamic trapping functions, while requiring simple fabrication process, and handling. Manuscript Click here to download Manuscript Arrays of high aspect ratio magnetic microstructures .pdf

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Micromagnet arrays enable precise manipulation of individual biological analyte–superparamagnetic bead complexes for separation and sensing

Cited by 40 publications

References 125 publications

Single-domain antibodies as promising experimental tools in imaging and isolation of porcine epidemic diarrhea virus

Single-domain antibodies as promising experimental tools in imaging and isolation of porcine epidemic diarrhea virus

The 2017 Magnetism Roadmap

Arrays of high aspect ratio magnetic microstructures for large trapping throughput in lab-on-chip systems

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