Micronized resveratrol shows promising effects in a seizure model in zebrafish and signalizes an important advance in epilepsy treatment

Decui, Laura; Garbinato, Cristiane; Schneider, Sabrina Ester; Mazon, Samara Cristina; Almeida, Emilia Rodrigues; Aguiar, Gean Pablo S.; Müller, Liz Girardi; Oliveira, J. Vladimir; Siebel, Anna Maria

doi:10.1016/j.eplepsyres.2019.106243

Cited by 30 publications

(12 citation statements)

References 36 publications

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“…The micronization of curcumin (Bertoncello et al, 2018), N-acetylcysteine (Aguiar et al, 2017), trans-resveratrol (Aguiar et al, 2018, 2016; Almeida et al, 2021; Decui et al, 2020), methotrexate (Chen et al, 2012), phenylbutazone (Moribe et al, 2005), Panax notoginseng saponins (Liang et al, 2021), etoposide (Cheng et al, 2016), ellagic acid (Li et al, 2015), taxifolin (Zu et al, 2012) atorvastatin calcium (Zhang et al, 2009) and ibuprofen (Han et al, 2011; Sosna et al, 2018) by the SEDS technique have shown a reduction in particle size, an increase in the dissolution rate, an increase in the solubility, as well as a modification of the crystalline structure of the compound when compared with non-micronized composts. Recently, the micronization of Panax notoginseng saponins changed the pharmacokinetic parameters of these compounds in rats, showing significantly higher values in plasma powders with smaller particle sizes than the larger particle sizes (Liang et al, 2021).…”

Section: Resultsmentioning

confidence: 99%

Curcumin micronization by supercritical fluid:in vitroandin vivobiological relevance

Sachett

Gallas‐Lopes

Benvenutti

et al. 2021

Preprint

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Curcumin, a polyphenol extracted from the rhizome of Curcuma longa L. (Zingiberaceae), is shown to have antioxidant, anti-inflammatory, neuroprotective, anxiolytic, and antidepressant properties in both preclinical and clinical studies. However, its low bioavailability is a limitation for its potential adoption as a therapeutic agent. The process of micronization can overcome this barrier by reducing the particle size and increasing the dissolution rate, potentially improving the bioavailability of the compounds of interest. In this study, we compared the in vitro antioxidant effects of curcumin (CUR) and micronized curcumin (MC) and studied their effects on behavioral and neurochemical parameters in zebrafish submitted to unpredictable chronic stress (UCS). MC (1 g/L) presented higher antioxidant activity in vitro as compared to CUR, as measured by iron-reducing antioxidant power (FRAP), 1,1-diphenyl-2-2-picyryl-hydrazyl radical removal (DPPH), and deoxyribose tests. UCS increased total distance traveled in the social interaction test (SI), while decreased crossings, time, and entries to the top area in the novel tank test (NTT). No effects of UCS were observed in the open tank test (OTT). The behavioral effects induced by UCS were not blocked by any curcumin preparation. UCS also decreased non-protein thiols (NPSH) levels, while increased glutathione reductase (GR) activity and thiobarbituric acid reactive substances (TBARS) levels on zebrafish brain. MC presented superior antioxidant properties than CUR in vivo, blocking the stress-induced neurochemical effects. Although this study did not measure the concentration of curcumin on the zebrafish brain, our results suggest that micronization increases the bioavailability of curcumin, potentiating its antioxidant activity both in vitro and in vivo. Our study also demonstrates that counteracting the oxidative imbalance induced by UCS is not sufficient to block its behavioral effects.

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Section: Resultsmentioning

confidence: 99%

Curcumin micronization by supercritical fluid:in vitroandin vivobiological relevance

Sachett

Gallas‐Lopes

Benvenutti

et al. 2021

Preprint

Self Cite

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“…The nervous system of the ZF is simple, with individual elements (glutamatergic excitatory neurons and GABAergic inhibitory neurons) [33]. Decui et al [34] have recently presented data that reveal that micronized resveratrol can prevent pentylenetetrazole (PTZ)-induced seizure in epilepsy, whereas results by Mazumder et al [35] have suggested that the anticonvulsant action of the phosphatidylinositol-3-kinase inhibitor LY294002 can be highly potential against PTZ-induced convulsions. Choo et al [36] have reported that newer antiepileptic drugs can trigger cognitive deficits in a PTZ-induced ZF model of epilepsy.…”

Section: Zf As Model For the Experimental Simulation Of Neurological Disordersmentioning

confidence: 99%

Zebrafish as an experimental model for the simulation of neurological and craniofacial disorders

et al. 2022

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Zebrafish have gained momentum as a leading experimental model in recent years. At present, the zebrafish vertebrate model is increasingly used due to its multifactorial similarities to humans that include genetic, organ, and cellular factors. With the emergence of novel research techniques that are very expensive, it is necessary to develop affordable and valid experimental models. This review aimed to highlight some of the most important similarities between zebrafish and humans by emphasizing the relevance of the first in simulating neurological disorders and craniofacial deformity.

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“…also been attributed to the usage of non-mammalian models in previous research as clinical trials cannot be conducted in humans due to ethical reasons. There are currently six non-mammalian models that have been used for epilepsy research which include roundworm (Locke et al, 2006), leech (Kristan et al, 2005, planaria, tadpole (Hewapathirane et al, 2008), fruit fly (Fogle et al, 2019) and zebrafish (Decui et al, 2020). Among all models, zebrafish has been widely utilized in epilepsy research due to the complex anatomy and behaviors that zebrafish possess including 70% similarity of its genome compared to humans (Johan Arief et al, 2018).…”

Section: Oryzias Latipes (Japanese Medaka) As Genetic Model To Study ...mentioning

confidence: 99%

Oryzias latipes (JAPANESE MEDAKA) AS GENETIC MODEL TO STUDY CAUSATIVE GENES OF EPILEPSY DISEASE: AN IN-SILICO APPROACH

Yunus

YUSUF

AHMAD

et al. 2022

MAB

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Epilepsy is a chronic neurological disorder that has affected around 50-70 million people worldwide. Various animal models have previously been used in epilepsy research. To expand the knowledge of the disease, a new animal model is suggested to be explored considering the genetic and phenotypic heterogeneity that contributes to the complexity of the disease. This study was undertaken to analyze 14 causative genes of epilepsy disease in Japanese medaka (Oryzias latipes), humans, and the established model of this disease which is zebrafish (Danio rerio) by assessing the variation in the genes by using MEGA X and predicting the functional motif and secondary structure of the proteins by using PROSITE and GORIV respectively. Results from the variation analysis showed the lowest percentage of conserved genes in Japanese medaka was 60%.50% of the genes of Japanese medaka were found to be more conserved than zebrafish in comparison to a human. The functional motifs present in all genes in Japanese medaka showed the same motifs present in humans. All the secondary structures of Japanese medaka genes were predicted to contain the alpha helix, extended strand, and random coil. In conclusion, it can be inferred that Japanese medaka could be a reliable animal model for epilepsy disease.

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Micronized resveratrol shows promising effects in a seizure model in zebrafish and signalizes an important advance in epilepsy treatment

Cited by 30 publications

References 36 publications

Curcumin micronization by supercritical fluid:in vitroandin vivobiological relevance

Curcumin micronization by supercritical fluid:in vitroandin vivobiological relevance

Zebrafish as an experimental model for the simulation of neurological and craniofacial disorders

Oryzias latipes (JAPANESE MEDAKA) AS GENETIC MODEL TO STUDY CAUSATIVE GENES OF EPILEPSY DISEASE: AN IN-SILICO APPROACH

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