Micronuclei frequency in peripheral blood lymphocytes of cancer patients: A meta-analysis

Iarmarcovai, G.; Ceppi, Marcello; Botta, A.; Orsière, Thierry; Bonassi, Stefano

doi:10.1016/j.mrrev.2008.05.006

Cited by 135 publications

(90 citation statements)

References 99 publications

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“…This result is in agreement with the previously published conclusions that the baseline MN frequency is higher in thyroid cancer patients (Iarmarcovai et al 2008) and in patients with other types of tumours (BaciuchkaPalmaro et al 2002;Bonassi et al 2007;Murgia et al 2008;Milošević-Djordjević et al 2010) than in healthy persons. Similarly, the increase in the mean MN frequency in PBLs of patients after 131-I therapy is consistent with the previous publications (Ballardin et al 2002;Popova et al 2005).…”

Section: Discussionsupporting

confidence: 93%

Correlation between Micronuclei Frequency in Peripheral Blood Lymphocytes and Retention of 131-I in Thyroid Cancer Patients

Vrndic

Milošević-Djordjević

Mijatovic-Teodorovic

et al. 2013

Tohoku J. Exp. Med.

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Differentiated thyroid cancers (DTCs) derive from thyroid follicular cells and include papillary and follicular cancers. In patients with DTCs, the initial treatment includes thyroidectomy and radioactive iodine (131-I) therapy. The objective of this study was to examine whether the intensity of DNA damage in peripheral blood lymphocytes (PBLs) of DTC patients depends on the amount of 131-I retained in the selected regions of interest (thyroid and abdominal region) as well as in the whole-body 72 hours after therapy. In addition, the possible influence of other factors that may affect micronuclei (MN) frequency, such as age, gender, smoking habits, and histological type of tumour was analyzed. The study population consisted of 22 DTC patients and 20 healthy donors. Data on the distribution of 131-I were obtained from the whole-body scans. MN frequency and cytokinesis-block proliferation index (CBPI) were measured using cytokinesis-block micronucleus assay. 131-I therapy significantly increased the MN frequency (19.50 ± 6.90 vs. 27.10 ± 19.50 MN) and significantly decreased the CBPI (1.52 ± 0.20 vs. 1.38 ± 0.17) in patients' lymphocytes. There was a clear correlation between the increased MN frequency and 131-I accumulation in the thyroid region in patients without metastases. The MN values did not differ in relation to the factors that could affect MN, such as age, gender, smoking habits, and histological type of tumour. In conclusion, the MN frequency in PBLs of DTC patients without metastases depends on the accumulation of 131-I in the thyroid region and does not depend on the other factors examined.

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Section: Discussionsupporting

confidence: 93%

Correlation between Micronuclei Frequency in Peripheral Blood Lymphocytes and Retention of 131-I in Thyroid Cancer Patients

Vrndic

Milošević-Djordjević

Mijatovic-Teodorovic

et al. 2013

Tohoku J. Exp. Med.

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“…Interestingly, the mean frequency of MN in the women with FM was 3.26-fold higher than the level seen in the healthy controls. In comparison, patients who have cancer have been noted to have 1.37-to 3.13-fold higher frequencies of MN when compared to healthy controls [57,58]. Given that the risk for cancer has been shown to be predicted by MN levels [37,41,58], the results of this preliminary data, if confirmed, suggest that MN frequency assessments may be useful for evaluating/diagnosing women with FM.…”

Section: Discussionmentioning

confidence: 62%

149. Epigenetic Alterations and an Increased Frequency of Micronuclei in Women With Fibromyalgia

Lyon

Menzies

Archer

et al. 2013

Brain, Behavior, and Immunity

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Fibromyalgia (FM), characterized by chronic widespread pain, fatigue, and cognitive/mood disturbances, leads to reduced workplace productivity and increased healthcare expenses. To determine if acquired epigenetic/genetic changes are associated with FM, we compared the frequency of spontaneously occurring micronuclei (MN) and genome-wide methylation patterns in women with FM ( = 10) to those seen in comparably aged healthy controls ( = 42 (MN); = 8 (methylation)). The mean (sd) MN frequency of women with FM (51.4 (21.9)) was significantly higher than that of controls (15.8 (8.5)) ( 2 = 45.552; df = 1; = 1.49 × 10 −11 ). Significant differences ( = 69 sites) in methylation patterns were observed between cases and controls considering a 5% false discovery rate. The majority of differentially methylated (DM) sites (91%) were attributable to increased values in the women with FM. The DM sites included significant biological clusters involved in neuron differentiation/nervous system development, skeletal/organ system development, and chromatin compaction. Genes associated with DM sites whose function has particular relevance to FM included BDNF, NAT15, HDAC4, PRKCA, RTN1, and PRKG1. Results support the need for future research to further examine the potential role of epigenetic and acquired chromosomal alterations as a possible biological mechanism underlying FM.

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“…After exposure, the pups were euthanized by decapitation for bone marrow extraction. Micronuclei induction was measured using the cell smear test with the bone marrow extracted from the femurs of rats as described by Iarmarcovai, Ceppi, Botta, Orsière, & Bonassi (2008) and Ribeiro, Salvadori, & Marques (2003). Using a microscope at 1000× magnification (Olympus CX 41), 1,000 polychromatic erythrocytes were counted in each slide, and the number of micronuclei observed was registered.…”

Section: Methodsmentioning

confidence: 99%

<b>Mutagenic effect of contaminated soil on the offspring of exposed rats

Garcia

Silva

Muccillo-Baisch

2016

Acta Sci. Health Sci.

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ABSTRACT. This study aimed to measure irreversible DNA damage in the offspring of rats exposed to contaminated soil. Bone marrow cells were removed from the femurs of the offspring of rats that were exposed to contaminated soil via gavage during three different periods: 21 days prior to detection of pregnancy, during pregnancy and 21 days after birth, during the lactation period. For each group, we also had a control group under the same experimental conditions but exposed to uncontaminated soil. The results showed that pups borne of rats exposed to contaminated soil had an increase in the number of micronuclei in the different exposure groups compared with their respective control groups. This suggests a potential risk of mutagenic damage to the offspring of pregnant women who live at the sampling site.Keywords: soil contamination, reproduction, wistar rats, metals, micronucleus.Efeito mutagênico da exposição a solos contaminados sobre a prole RESUMO. Este estudo objetivou medir os danos irreversíveis de DNA na prole de ratas expostas a um solo contaminado, através do teste do micronúcleo. O mesmo foi realizado na medula óssea retirada do fêmur de filhotes de ratas expostas, via gavagem, em três diferentes períodos, ratas expostas durante 21 dias antes da detecção da prenhez, durante a prenhez e 21 dias após o nascimento, durante o período de aleitamento. Para cada grupo foi utilizado um grupo controle, mantido nas mesmas condições experimentais e expostos a um solo não contaminado. Os resultados obtidos nos filhotes expostos ao solo contaminado mostraram um aumento no número de micronúcleos nos três diferentes grupos expostos quando comparados aos seus respectivos grupos controle. Assim, os dados sugerem um potencial risco de dano mutagênico aos descendentes das gestantes residentes no local de amostragem.Palavras-chave: contaminação terrestre. reprodução. ratas wistar. elementos metálicos. micronúcleo.

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Micronuclei frequency in peripheral blood lymphocytes of cancer patients: A meta-analysis

Cited by 135 publications

References 99 publications

Correlation between Micronuclei Frequency in Peripheral Blood Lymphocytes and Retention of 131-I in Thyroid Cancer Patients

Correlation between Micronuclei Frequency in Peripheral Blood Lymphocytes and Retention of 131-I in Thyroid Cancer Patients

149. Epigenetic Alterations and an Increased Frequency of Micronuclei in Women With Fibromyalgia

<b>Mutagenic effect of contaminated soil on the offspring of exposed rats

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