Micronuclei in diabetes: Folate supplementation diminishes micronuclei in diabetic patients but not in an animal model

Zúñiga‐González, Guillermo Moisés; Batista-González, Cecilia M.; Gómez‐Meda, Belinda Claudia; Ramos-Ibarra, María Luisa; Zamora‐Pérez, Ana Lourdes; Muñoz-Magallanes, T.; Ramos-Valdés, Carmen; Gallegos‐Arreola, Martha Patricia

doi:10.1016/j.mrgentox.2007.06.006

Cited by 58 publications

(35 citation statements)

References 41 publications

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“…Previous studies reported that FA decreased the formation of MN [4,8,10,15,33]. In this study, the frequencies of cells with NAs and MN were significantly higher in patients with diabetes than in healthy controls.…”

Section: Dm Genotoxicity Was Studied By Analysis Of Nas In the Oral Msupporting

confidence: 60%

“…The smears were air-dried, fixed in 80% methanol for 48 hours, and stained with acridine orange [8,23].…”

Section: Sample Preparation and Analysismentioning

confidence: 99%

“…Increased production of free radicals and reactive oxygen species (ROS) causes oxidative damage to biomolecules [1,3,[8][9][10][11]. ROS can damage genetic material, resulting in abnormalities in the integrity of the cell nucleus and DNA breakage, producing micronuclei (MN) [8][9][10][12][13][14][15][16]. Antioxidants can counteract DNA damage generated by free radicals in patients with diabetes and other conditions [3,4,8,10,[17][18][19].…”

Section: Introductionmentioning

confidence: 99%

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Nuclear abnormalities in buccal mucosa cells of patients with type I and II diabetes treated with folic acid

Gómez‐Meda

Zamora‐Pérez

Muñoz-Magallanes

et al. 2016

Mutation Research/Genetic Toxicology and Environmental Mutagene

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Section: Dm Genotoxicity Was Studied By Analysis Of Nas In the Oral Msupporting

confidence: 60%

“…The smears were air-dried, fixed in 80% methanol for 48 hours, and stained with acridine orange [8,23].…”

Section: Sample Preparation and Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Nuclear abnormalities in buccal mucosa cells of patients with type I and II diabetes treated with folic acid

Gómez‐Meda

Zamora‐Pérez

Muñoz-Magallanes

et al. 2016

Mutation Research/Genetic Toxicology and Environmental Mutagene

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“…The levels of buccal MN in diabetic individuals are approximately 2-fold higher than in nondiabetic participants, as revealed by Elisabeth mullner et al [18]. Zuniga-Gonzalez et al stated that the increased frequency of MN levels in exfoliated buccal cells in type1 and type 2 diabetic patients compared with control group [21]. Thus these findings support our study.…”

Section: Discussionsupporting

confidence: 91%

“…Hence it can be stated that MN may be a useful constituent in a panel of biomarkers for the risk of diabetes and its complications [16]. Few studies have investigated DNA damage by MN in patients with type 2 diabetes, and these studies have shown increased frequency of micronuclei in diabetic patients [16,21]. It is worth mentioning that the authors themselves highlighted the need to conduct further studies to validate this investigation.…”

Section: Introductionmentioning

confidence: 99%

Micronuclei in exfoliated buccal cells: a biomarker for dna damage leading to progression of late diabetic complications in type 2 diabetes mellitus patients

Biswas¹,

Banerjee²,

Bhattacharya³

et al. 2017

Int j curr adv res

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Elevated levels of DNA damage, decreased efficacy of DNA repair and high susceptibility to mutagens have been associated with type 2 diabetes mellitus. Micronuclei (MN) are a sensitive indicator of genetic damage and a marker of DNA damage. MN is also a morphological marker of chromosomal instability. So the presence of MN in cells reflects structural chromosomal aberrations arising during mitosis. Our aim was to evaluate the frequencies of micronuclei, as a biomarker for DNA damage, in Giemsa stained buccal cells of T2DM patients and healthy controls. A case-control study of seventy six patients with T2DM and twenty five control group participants were recruited from different areas of West Bengal for determination of frequency of MN. All procedures were done with the informed consent of participants. In the present study, frequency of MN found in T2DM patients was 4.08±3.77, which was much higher than the control group participants (1.1±0.9). Frequency of MN increased due to the elevated level of DNA damage in these patients than the controls. Hence, in the present study, increased DNA damage may be due to increased oxidative stress leading to genomic instability, which in turn may lead to the progression of T2DM and its complications and contribute to an increased risk for cancer. This depicts that MN may be a useful constituent in a panel of biomarkers for the risk of late diabetic complications and can be a predisposing factor for development of cancer. INTRODUCTIONType 2 diabetes mellitus (T2DM), the most frequent subtype of diabetes, comprises a complex heterogeneous group of metabolic diseases characterized by high levels of blood glucose (hyperglycaemia) and impaired insulin action and/or insulin secretion.The number of people with T2DM is increasing in every country with 80% of population with DM living in low-and middle-income countries [1]. It is estimated that T2DM affects about 150 million people worldwide and it is expected to rise to about 439 million people by the year 2030 [2,3].India ranked first in the world for the prevalence of the disease, followed by China and USA [4].There are currently approximately 40.9 million patients suffering from diabetes mellitus in India and it is expected to rise to about 69.9 million by the year 2025 [5]. Anticipating an epidemic like increase in the number of diabetic patients, India is being referred to as the 'diabetic capital of the world [6]. About 31.0% populations of West Bengal are suffering from T2DM [7]. The incidence of T2DM varies substantially from one geographical region to the other as a result of environmental and lifestyle risk factors [2]. One of the factors associated with diabetic complications is the process of non-enzymatic glycation of proteins, lipids and nucleic acids, with subsequent formation of advanced glycation end products (AGEs) [8]. One of the main consequences of this adverse action is the formation of oxidative stress (OS). Formation of glycation end products leads to OS and subsequent DNA damage via the oxidation of DNA base...

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Micronucleated erythrocytes in peripheral blood from neonate rats fed by nursing mothers exposed to X‐rays

Solórzano-Meléndez

Rodrigo‐Alarcón

Gómez‐Meda

et al. 2021

Environ and Mol Mutagen

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Most women with breast cancer can become pregnant and give birth while undergoing radiation therapy and breastfeeding is generally not contraindicated. The induction of long‐lived reactive species in proteins, such as casein by X‐ray radiation and DNA damage to unexposed organisms, has been shown when ingesting irradiated cheese. To determine whether exposing lactating rats to X‐rays increases the number of micronucleated erythrocytes (MNEs) in peripheral blood of their unexposed or breastfeeding rat pups, 15 female Wistar rats were divided into three groups: Negative control; Experimental group exposed to X‐rays, and group exposed to X‐rays plus vitamin C. The mothers of groups 2 and 3 were irradiated for three consecutive days after giving birth, returning them to their respective cages each time to continue lactation. A blood sample was taken from the mothers and pups at 0, 24, and 48 hr. Blood smears were stained with acridine orange to analyze MNEs. In mother rats, the frequency of micronucleated polychromatic erythrocytes (MNPCEs) increased significantly at 24 and 48 hr in both study groups exposed to radiation. Likewise, in rat pups the MNPCE and MNE frequencies increased in both groups with radiation and radiation plus vitamin C at 24 and 48 hr, and a protection from vitamin C was observed. In conclusion, the genotoxic damage produced in rat pups that were lactated by mothers irradiated with X‐rays is possibly due to the effect of long‐lived reactive species that were formed in the breast milk of female Wistar rats during the irradiation process.

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Micronuclei in diabetes: Folate supplementation diminishes micronuclei in diabetic patients but not in an animal model

Cited by 58 publications

References 41 publications

Nuclear abnormalities in buccal mucosa cells of patients with type I and II diabetes treated with folic acid

Nuclear abnormalities in buccal mucosa cells of patients with type I and II diabetes treated with folic acid

Micronuclei in exfoliated buccal cells: a biomarker for dna damage leading to progression of late diabetic complications in type 2 diabetes mellitus patients

Micronucleated erythrocytes in peripheral blood from neonate rats fed by nursing mothers exposed to X‐rays

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