Micronucleus and its applications

Samanta, Samiran; Dey, Pranab

doi:10.1002/dc.21592

Cited by 108 publications

(124 citation statements)

References 54 publications

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“…34 The micronuclear test being the most sensitive and the most powerful statistical tool to evaluate DNA breaks, which indicate potential mutations. 35 Meanwhile, Results showed that three types of honey, which used in this study formed by different feed sources (HS, HW and HF, had no significant effect on the induction of micronuclei in bone marrow cells, which showed nearly the same values of control as shown in Table 1.…”

Section: Resultsmentioning

confidence: 99%

Investigation of role magnetized water used in supplementary feeding for honeybees to modulate the genotoxic side effects induced by cyclophosphamide in mice bone marrow cells

Farhan¹,

Chechan

Alkinani

2017

J. contemp. med. sci.

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Objective To evaluate the possible protective role of honey formed through innovative way by using magnetized water instead of tab water in supplementary feeding for bees (HW) and compared with two honey types formed by two different feeding solutions; sugar syrup (HS) and nectar of flowers (HF) against cyclophosphamide (CP) genotoxicity in mice bone marrow cells, by using the micronucleus assay (MN). Methods Mice were divided into 8 groups from 3 animals each. G1 as control. G2 were exposed to CP (40 mg/kg), G3, G4, and G5 received two dose of three types of honey at doses (300, 600 mg/kg) respectively. The other groups G6, G7 and G8 were supplemented with three types honey (300 mg/kg) in three different experimental protocols, as pre 2 h, post 2 h, and concomitant treatment for 7 and 14 days. Results Examination and analysis of MN showed no mutagenic effect of three types honey per se doses, especially in low dose (300 mg/kg). Meanwhile, CP induced a significant (P < 0.01) increase in MN frequency. While dual treatment with groups of honey HW caused a significant reduction in MN induced by CP in bone marrow cells in a time-dependant manner. Also, the results confirmed the protective efficacy of HW group and/or HF group as compared with HS group, against CP-toxicity. Conclusion Our study suggests using magnetized water in supplementary feeding of bee, that could give the honey protective effect against genotoxicity induced by CP, it is also fosters antioxidant activity of honey constituents. Therefore, honey HW can be used as an adjuvant with chemotherapeutic agents for minimizing the genotoxic side effects of the anticancer drug CP.

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Section: Resultsmentioning

confidence: 99%

Investigation of role magnetized water used in supplementary feeding for honeybees to modulate the genotoxic side effects induced by cyclophosphamide in mice bone marrow cells

Farhan¹,

Chechan

Alkinani

2017

J. contemp. med. sci.

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“…The comparison indicates that micronuclei formation in epithelial cells may be overestimated with Pap stain. 11 However, the Pap stain is still being used. The reasons to use Papanicolaou stain in this study were simple to use, less time consuming, and economical.…”

Section: Discussionmentioning

confidence: 99%

“…The MN test is a noninvasive, cost-effective, and easily acceptable procedure by patients. [10][11][12] Based on this background, this study was undertaken to identify the feasible and economical method that could be used as a screening test in high-risk population for identifying the effects of genomic instabilities.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Micronuclei in Oral Squamous Cell Carcinoma: A Cytological Study

Chaudhary¹,

Ramesh²,

Oza³

et al. 2017

ijocrweb

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“…Conventionally prepared and May-Grünwald-Giemsa (MGG) stained slides were used for MN counting. For the evaluation of MN, we used the following preestablished criteria: a diameter smaller than 1/3 of the main nucleus, round/oval shape with regular contours, same staining intensity with the main nucleus, and no connection to the main nucleus (3,17) (Figure).…”

Section: Methodsmentioning

confidence: 99%

Increased micronucleus count predicts malignant behavior in pleural e usion uid

2018

Turk J Med Sci

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Background/aim: Micronucleus (MN) frequency is used as a biomarker of chromosomal damage, genome instability, and cancer risk. The aim of this study was to evaluate the diagnostic usefulness of MN frequency to differentiate between malignant and benign pleural effusion samples.Materials and methods: Retrospectively, 78 pleural fluid cytology samples (including 20 cases of benign reactive mesothelial cells, 22 cases of suspicious cytology, and 36 cases of malignant cytology) were examined. The number of micronucleated cells in 1000 wellpreserved cells was counted. Statistical tests were performed to compare the study groups. Recover operating characteristics (ROC) curve analysis was performed to suggest a cut-off value for predicting malignant behavior. Results:We evaluated a total of 78 cases of pleural effusion cytology. The number of micronucleated cells was significantly higher in cases with malignant outcome compared to cases with benign outcome. We observed that malignant samples had more micronucleated cells than suspicious ones, and suspicious cases had more micronucleated cells than reactive ones. There was a significant difference among all study groups. In addition, the frequency of MN-containing cells in suspicious cases correlates well with their outcomes. Conclusion:The results of this study reveal that there is an absolute, consistent, and proportional relationship between MN counts and malignancy in cytological samples of pleural effusions. MN scoring may be a helpful diagnostic tool for distinguishing malignant effusions from benign ones, and may be used as an adjunct tool to predict malignant behavior in challenging cases.

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Micronucleus and its applications

Cited by 108 publications

References 54 publications

Investigation of role magnetized water used in supplementary feeding for honeybees to modulate the genotoxic side effects induced by cyclophosphamide in mice bone marrow cells

Investigation of role magnetized water used in supplementary feeding for honeybees to modulate the genotoxic side effects induced by cyclophosphamide in mice bone marrow cells

Evaluation of Micronuclei in Oral Squamous Cell Carcinoma: A Cytological Study

Increased micronucleus count predicts malignant behavior in pleural e usion uid

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