Micronucleus scoring: An available approach in the evaluation of genomic damage in exfoliative cervicovaginal cells

Öz, Zehra Safi

doi:10.17352/acp.000018

Cited by 4 publications

(3 citation statements)

References 28 publications

(38 reference statements)

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“…MN'lar, hücrenin mitoz bölünmesi sırasında serbest kalan kromozom fragmentinin ya da kromozomların bir zarla çevrilmesi ile oluşan ve ana nükleusun yanında yer alan yapılardır. MN'ler pek çok farklı yöntem ile gösterilebilmektedir (14)(15)(16). DNA hasarı pekçok moleküler yöntemle gösterilmekte ancak bu yöntemler oldukça pahalı ekipmanları gerektirmektedir (17).…”

Section: Introductionunclassified

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Evaluation of Micronucleer and Binucleer Cells Frequencies in Buccal Epithelial Cells of Children Infected with Helicobacter pylori

Öz

Üstündağ

ALTUNTAŞ

et al. 2023

Batı Karadeniz Tıp Dergisi

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Amaç: Helicobacter pylori (H. pylori) gastroduodenal inflamasyon, ülser ve atrofik gastrite yol açan bakteriyel bir patojendir. Mikronüklus (MN)’ lar hücrenin mitoz bölünmesi sırasında ortaya çıkan esas çekirdeğe dahil olmayan oluşumlardır. Bu çalışmada, H. pylori ile mikronükleer ve binükleer hücre arasındaki ilişkinin eksfolyatif sitoloji yöntemi ile değerlendirilmesi amaçlanmıştır. Gereç ve Yöntemler: Bu çalışmada Zonguldak Bülent Ecevit Üniversitesi Uygulama ve Araştırma Hastanesi Çocuk Gastroenteroloji polikliniğine yaşam kalitesini etkileyecek tarzda ciddi dispeptik yakınmalarla başvuran ve endoskopik biyopsi yapılan hastalar yer aldı. Biyopsi örnekleri formalinde fikse edildi ve parafin bloklardan hazırlanan kesitler Hematoksilen & Eozin (H&E) ile boyandı. H. pylori varlığı açısından ışık mikroskobik olarak değerlendirildi. Biyopsi sonucu sadece H. pylori pozitif örnekler çalışma grubu (n=30) hiçbir enfeksiyon etkeni saptanmayanlar ise kontrol grubu (n=30) olarak alındı. Tüm hastalardan alınan yanak epitel hücreleri lamlara yayıldı, % 95’lik etil alkolde fikse edildi ve Papanicolaou boyama yöntemine göre boyandı. Mikronükleer ve binükleer hücreler iyi boyanmış 1000 epitel hücrede sayıldı. İstatistiksel değerlendirme SPSS 18.0 (SPSS Inc., Chicago, IL, USA) programı kullanıldı ve p

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Section: Introductionunclassified

“…MN'lerin ışık mikroskobik olarak değerlendirildiği yöntemlerden biri de eksfolyatif sitoloji yöntemidir. Eksfolyatif sitoloji dökülen hücrelerin alınarak lamlar üzerine yayılması ve özel boyalarla boyanıp mikroskopik olarak incelenmesi esasına dayanmakta olup yanak ve dil epitel hücrelerine de uygulanabilmektedir (14)(15)(16)(17)(18).…”

Section: Introductionunclassified

Evaluation of Micronucleer and Binucleer Cells Frequencies in Buccal Epithelial Cells of Children Infected with Helicobacter pylori

Öz

Üstündağ

ALTUNTAŞ

et al. 2023

Batı Karadeniz Tıp Dergisi

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“…The existence of MN indicates genomic instability due to chromosomal aberration and represents cancer risk. In most cancers, the number of micronuclei increases over a while [4].…”

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confidence: 99%

Connotation of Micronucleus as a Biomarker in Cervical Intraepithelial Lesions and Carcinoma

Devendrappa,

Anantharaj,

Sangita Ravindra

et al. 2023

IJAR

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Introduction: The Pap smears study is the most rampant and cost-effective method for cervical cancer screening. The role of micronuclei in exfoliated cells of pap smears is the greater beneficial tool used in the screening of cervical cancers. They are intracytoplasmic inclusions made up of chromatin fragments that suggest instability in the chromosomes and they are found in great numbers in carcinogen-exposed tissues long before any clinical signs occur. Aims: To evaluate the predominance of Micronuclei as a biomarker in various premalignant and malignant lesions of cervixand to explore the association between various grades of cervical lesions and micronuclei frequency. Materials &Methods:A prospective study was done in the cytopathology section on 250 cervical pap smears collected in the Pathology laboratoryRajarajeswari medical college, Bangalore. The study period was from January 2021 to June 2021. Slides containing the cell material were stained with Papanicolaou stain, The results were analyzed as per the Micronuclei Scoring done per 1000 exfoliated squamous cells under light microscopy Results: In this study of 250 cervical pap smear screening, there was a stepwise gradual increase in micronuclei count(MN score)from Inflammatory lesions to carcinoma cervix. Inflammatory 2.04 Â± 0.43, Atypicalsquamous cells of undetermined significance 3.9 Â± 1.73, Atypical squamous cells - High grade5.64 Â±1.83,Low grade squamous intraepithelial lesion 7.68 Â±1.48, High grade squamous intraepithelial lesion08 12.32 Â± 2.68and Intraepithelial Carcinoma 18.60 Â± 2.83. Conclusion: MN scoring on cervix squamous epithelial cells is a simple and reliable objective test that may be performed on routine Pap-stained smears and can be used as adjunctive to the screening of cervical cancers and provide a potential pathway for disease monitoring in the future.

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Cytomorphometry and morphology analysis of human papillomavirus type 16 in liquid‐based cervical cytology samples

Öz

Kandemır

Köktürk

et al. 2021

Diagnostic Cytopathology

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BackgroundHuman papillomavirus (HPV) is the main causal factor of cervical carcinoma. HPV 16 is one of the most prominent oncogenic types. We aimed to evaluate the cytomorphometric and morphological alterations caused by HPV 16 in liquid‐based cytology (LBC).MethodsThe Cobas 4800 HPV system was used for the detecting and typing HPV DNA in cervical specimens. In this study, 30 HPV 16 positive and 30 HPV 16 negative cervical samples were evaluated for micronuclei (MN), nonclassical cytologic abnormalities, and the nuclear‐to‐cytoplasmic ratio. Nuclear and cellular areas were evaluated using image analysis software and the nuclear‐to‐cytoplasm ratio was calculated. All analyses were performed blinded to the patients' HPV status. Statistical evaluation was carried out using the χ2 and Fisher test; P‐values < .05 were considered significant.ResultsThe frequencies of micronucleated cells and koilocytes were higher in the HPV 16 infected group (P < .05). Cells with perinuclear halo in control group were higher than the HPV 16 infected group (P < .05). The mean nuclear‐to‐cytoplasm ratio in HPV 16 patients was higher than the control value, but the difference was not statistically significant.ConclusionLBC can be used to detect morphological and morphometric changes. HPV 16 induces the formation of MN and koilocytosis. The evaluation of MN could provide additional information in monitoring genomic instability and of koilocytes could provide information about damage to the cytoskeleton filaments in HPV infection. Further studies are needed to investigate the effects of HPV‐18 and other high‐risk HPV types on the cell size and nucleus‐to‐cytoplasm ratio.

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Micronucleus scoring: An available approach in the evaluation of genomic damage in exfoliative cervicovaginal cells

Cited by 4 publications

References 28 publications

Evaluation of Micronucleer and Binucleer Cells Frequencies in Buccal Epithelial Cells of Children Infected with Helicobacter pylori

Evaluation of Micronucleer and Binucleer Cells Frequencies in Buccal Epithelial Cells of Children Infected with Helicobacter pylori

Connotation of Micronucleus as a Biomarker in Cervical Intraepithelial Lesions and Carcinoma

Cytomorphometry and morphology analysis of human papillomavirus type 16 in liquid‐based cervical cytology samples

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