Microorganisms as phytase producers

Mukhametzyanova, A. D.; Akhmetova, Irina; Шарипова, М. Р.

doi:10.1134/s0026261712030095

Cited by 44 publications

(32 citation statements)

References 47 publications

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“…Phytases are synthesized by many microorganisms, including various bacteria, fungi, micromycetes and other microbes often collectively called biofertilizers due to their ability to promote plant growth. Secreted microbial phytases hold a high potential for biotechnology due to their often higher specific activity towards phytate [16] [17] and can potentially be used to increase soil phosphorus availability for plant nutrition. In light of this, a promising approach in plant biotechnology is to generate transgenic plants engineered to secrete microbial phytases into rhizosphere.…”

Section: Introductionmentioning

confidence: 99%

Microbial Phytases and Phytate: Exploring Opportunities for Sustainable Phosphorus Management in Agriculture

Balaban¹,

Suleimanova²,

Valeeva³

et al. 2017

AJMB

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Myo-inositol phosphates (phytates) are important biological molecules produced largely by plants to store phosphorus. Phytate is very abundant in many different soils making up a large portion of all soil phosphorus. This review assesses current phytase science from the perspective of its substrate, phytate, by examining the intricate relationship between the phytate-hydrolyzing enzymes and phytate as their substrate. Specifically, we examine available data on phytate's structural features, distribution in nature and functional roles. The role of phytases and their localization in soil and plant tissues are evaluated. We provide a summary of the current biotechnological advances in using industrial or recombinant phytases to improve plant growth and animal nutrition. The prospects of future discovery of novel phytases with improved biochemical properties and bioengineering of existing enzymes are also discussed. Two alternative but complementary directions to increase phosphorus bioavailability through the more efficient utilization of soil phytate are currently being developed. These approaches take advantage of microbial phytases secreted into rhizosphere either by phytase-producing microbes (biofertilizers) or by genetically engineered plants. More research on phytate metabolism in soils and plants is needed to promote environmentally friendly, more productive and sustainable agriculture.

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Section: Introductionmentioning

confidence: 99%

Microbial Phytases and Phytate: Exploring Opportunities for Sustainable Phosphorus Management in Agriculture

Balaban¹,

Suleimanova²,

Valeeva³

et al. 2017

AJMB

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“…The model was able to distinguish between these two different forms of P excreted. The insoluble P is only found in the faeces and is composed of oP (mainly in IP 6 , but also IP 3 -IP 5 forms) and any divalent cation complexes (Selle et al, 2011;Mukhametzyanova et al, 2012). Soluble P excretion originates from urinary P excretion, and contains a fraction of soluble P excreted in the faeces, see equation (6).…”

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Modelling phosphorus intake, digestion, retention and excretion in growing and finishing pigs: model description

Symeou

Leinonen

Kyriazakis

2014

Animal

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Low phosphorus (P) digestibility combined with intensive pig production can increase P diffuse pollution and environmental load. The aim of this paper was to develop a deterministic, dynamic model able to represent P digestion, retention and ultimately excretion in growing and finishing pigs of different genotypes, offered access to diets of different composition. The model represented the limited ability of pig endogenous phytase activity to dephosphorylate phytate as a linear function of dietary calcium (Ca). Phytate dephosphorylation in the stomach by exogenous microbial phytase enzymes was expressed by a first order kinetics relationship. The absorption of non-phytate P from the lumen of the small intestine into the blood stream was set at 0.8 and the dephosphorylated phytate from the large intestine was assumed to be indigestible. The net efficiency of using digested P was set at 0.94 and assumed to be independent of BW, and constant across genotype and sex. P requirements for both maintenance and growth were made simple functions of body protein mass, and hence functions of animal genotype. Undigested P was assumed to be excreted in the feaces in both soluble and insoluble forms. If digestible P exceeded the requirements for P then the excess digestible P was excreted through the urinary flow; thus the model represented both forms of P excretion (soluble and insoluble) into the environment. Using a UK industry standard diet, model behaviour was investigated for its predictions of P digestibility, retention and excretion under different levels of inclusion of microbial phytase and dietary Ca, and different non-phytate P : phytate ratios in the diet, thus covering a broad space of potential diet compositions. Model predictions were consistent with our understanding of P digestion, metabolism and excretion. Uncertainties associated with the underlying assumptions of the model were identified. Their consequences on model predictions, as well as the model evaluation are assessed in a companion paper.Keywords: modelling, phosphorus, phytase enzymes, phytate, pig ImplicationsCurrently there is some disagreement about the phosphorus (P) requirements of pigs of different genotypes and how digestible P contents of pig diets are calculated, especially for diets that include different amounts of phytate and nonphytate P. Achieving a balance between meeting digestible P requirements for optimum growth and health, and avoiding excess P intake would lead to a reduction in diffuse P levels in manure and effluents, and environmental impact from pig systems. A simulation model that predicts P intake, digestion, retention and excretion is the first, necessary step towards achieving this aim. IntroductionPhosphorus (P) is an important mineral for both the metabolism and skeletal development of the growing pig (NRC, 2012).In pig diets, P is the third most expensive nutrient required, after carbohydrates (energy) and protein. The high cost of P is due to the low digestibility of plant dietary P, which results in the need to sup...

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“…Phytases comprise a specific group of phosphatases which are capable of hydrolyzing myo-inositol 1,2,3,4,5,6-hexakisphosphate (phytate) with the formation of less phosphorylated inositol derivatives (from mono-to pentaphosphate) (7). A number of phytases have been identified in plants, microorganisms, and some animal tissues (8).…”

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confidence: 99%

Novel Glucose-1-Phosphatase with High Phytase Activity and Unusual Metal Ion Activation from Soil Bacterium Pantoea sp. Strain 3.5.1

Suleimanova

Beinhauer

Valeeva

et al. 2015

Appl Environ Microbiol

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Phosphorus is an important macronutrient, but its availability in soil is limited. Many soil microorganisms improve the bioavailability of phosphate by releasing it from various organic compounds, including phytate. To investigate the diversity of phytate-hydrolyzing bacteria in soil, we sampled soils of various ecological habitats, including forest, private homesteads, large agricultural complexes, and urban landscapes. Bacterial isolate Pantoea sp. strain 3.5.1 with the highest level of phytase activity was isolated from forest soil and investigated further. The Pantoea sp. 3.5.1 agpP gene encoding a novel glucose-1-phosphatase with high phytase activity was identified, and the corresponding protein was purified to apparent homogeneity, sequenced by mass spectroscopy, and biochemically characterized. The AgpP enzyme exhibits maximum activity and stability at pH 4.5 and at 37°C. The enzyme belongs to a group of histidine acid phosphatases and has the lowest K m values toward phytate, glucose-6-phosphate, and glucose-1-phosphate. Unexpectedly, stimulation of enzymatic activity by several divalent metal ions was observed for the AgpP enzyme. High-performance liquid chromatography (HPLC) and high-performance ion chromatography (HPIC) analyses of phytate hydrolysis products identify DL-myo-inositol 1,2,4,5,6-pentakisphosphate as the final product of the reaction, indicating that the Pantoea sp. AgpP glucose-1-phosphatase can be classified as a 3-phytase. The identification of the Pantoea sp. AgpP phytase and its unusual regulation by metal ions highlight the remarkable diversity of phosphorus metabolism regulation in soil bacteria. Furthermore, our data indicate that natural forest soils harbor rich reservoirs of novel phytate-hydrolyzing enzymes with unique biochemical features. P hosphorus is one of the most important macroelements of living cells. It is a crucial component of nucleic acids, highenergy compounds, phospholipids, and other molecules and is essential for normal plant growth. Many soil types are naturally low in inorganic phosphorus, and, as the pressure on agriculture to provide food for the growing human population intensifies, so does the need for a massive use of phosphate fertilizers. However, rock phosphate is a nonrenewable natural resource (1). Furthermore, the vast majority of applied phosphate fertilizer quickly interacts with soil components, leading to its rapid transformation into various organic or precipitated compounds inaccessible to plants. Thus, much attention has recently been paid to alternative sources of phosphorus in soil.Organic phosphorus compounds in soil account for 30 to 50% of total soil phosphorus and are often considered natural alternatives to the use of mineral phosphate fertilizer (2). Phytate is a molecule of myo-inositol with six phosphate residues (3). Phytate concentration varies from 3.9% to 25.3% of total extractable P in carbonate-free cambisol soils and calcareous chernozems, respectively (4), suggesting that in some cases phytate can be one of the major for...

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Microorganisms as phytase producers

Cited by 44 publications

References 47 publications

Microbial Phytases and Phytate: Exploring Opportunities for Sustainable Phosphorus Management in Agriculture

Microbial Phytases and Phytate: Exploring Opportunities for Sustainable Phosphorus Management in Agriculture

Modelling phosphorus intake, digestion, retention and excretion in growing and finishing pigs: model description

Novel Glucose-1-Phosphatase with High Phytase Activity and Unusual Metal Ion Activation from Soil Bacterium Pantoea sp. Strain 3.5.1

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