Microorganisms Involved in Bioleaching and Nucleic Acid-Based Molecular Methods for Their Identification and Quantification

Schippers, Axel

doi:10.1007/1-4020-5589-7_1

Cited by 105 publications

(81 citation statements)

References 190 publications

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“…caldus, L. ferriphilum, and Sb. thermosulfidooxidans were among the most common species found in stirred tank processes in mesophilic conditions (Olson et al 2003;Dopson and Lindström 2004;Shippers 2007). The reduced number of detected strains demonstrated once more the limited diversity of consortia that thrive in bioleaching processes (Okibe et al 2003).…”

Section: Discussionmentioning

confidence: 99%

“…Molecular ecology as a means of monitoring bacterial community structure gives new insights into bioleaching processes. The techniques available to carry out molecular microbial ecology are varied and have been reviewed extensively (Johnson and Hallberg 2007;Shippers 2007). They comprise CE-SSCP (Foucher et al 2003), DGGE (Demergasso et al 2005;Halinen et al 2009), real-time quantitative PCR (Zhang et al 2009.…”

Section: Introductionmentioning

confidence: 99%

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Relationship between bioleaching performance, bacterial community structure and mineralogy in the bioleaching of a copper concentrate in stirred-tank reactors

Spolaore

Joulian

Gouin

et al. 2010

Appl Microbiol Biotechnol

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Hugues. Relationship between bioleaching performance, bacterial community structure and mineralogy in the bioleaching of a copper concentrate in stirred-tank reactors. Applied Microbiology and Biotechnology, Springer Verlag, 2011, 89 (2) The experiments undertaken to produce the techno-economic data were also an opportunity to carry out more fundamental research. The objective of this work was to combine the results of the bioleaching experiments, in terms of copper recovery, with the results of bacterial community monitoring and mineralogy residue analysis.Batch and continuous bioleaching tests were carried out with 10% solids, at 42 °C and with a pH between 1.2 and 1.6. Final copper recovery was higher in batch cultures than in continuous mode (>95% vs. 91%). Mineralogical analysis showed that the limiting factor for copper recovery was incomplete chalcopyrite dissolution in both cases. However, chalcopyrite was even less dissolved in continuous conditions. This was also related to a variation in bacterial community structure. The population in all tests was composed of Acidithiobacillus caldus, Leptospirillum ferriphilum and one or two species of Sulfobacillus (thermosulfidooxidans and sometimes benefaciens) but Sulfobacillus and more generally sulphur oxidizers were more represented in batch mode.It was proposed that, due to their capacity to reduce inorganic compounds, sulphur oxidizers may be efficient in limiting chalcopyrite surface hindering. It may help to better dissolve this mineral and reach a better copper recovery.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Relationship between bioleaching performance, bacterial community structure and mineralogy in the bioleaching of a copper concentrate in stirred-tank reactors

Spolaore

Joulian

Gouin

et al. 2010

Appl Microbiol Biotechnol

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“…When Fe(III) (hydr)oxides are dissolved, adsorbed or precipitated metals are released. Sulfate-reducing Bacteria or Archaea may also precipitate metals as metal sulfides (22,49,50).…”

Section: ϫmentioning

confidence: 99%

“…When Fe(III) (hydr)oxides are dissolved, adsorbed or precipitated metals are released. Sulfate-reducing Bacteria or Archaea may also precipitate metals as metal sulfides (22,49,50).Primarily cultivation techniques have been used to enumerate prokaryotes involved in oxidation and reduction processes in sulfidic mine waste dumps (3,17,52,57,64). Cultivation techniques yield cell numbers merely according to physiological properties; therefore, only a subset of the whole microbial community is detected.…”

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confidence: 99%

Quantitative Microbial Community Analysis of Three Different Sulfidic Mine Tailing Dumps Generating Acid Mine Drainage

Kock

Schippers

2008

Appl Environ Microbiol

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The microbial communities of three different sulfidic and acidic mine waste tailing dumps located in Botswana, Germany, and Sweden were quantitatively analyzed using quantitative real-time PCR (Q-PCR), fluorescence in situ hybridization (FISH), catalyzed reporter deposition-FISH (CARD-FISH), Sybr green II direct counting, and the most probable number (MPN) cultivation technique. Depth profiles of cell numbers showed that the compositions of the microbial communities are greatly different at the three sites and also strongly varied between zones of oxidized and unoxidized tailings. Maximum cell numbers of up to 10 9 cells g ؊1 dry weight were determined in the pyrite or pyrrhotite oxidation zones, whereas cell numbers in unoxidized tailings were significantly lower. Mining residues from mining activities are dumped as waste rock or as tailings, which are metal-degraded materials from ore processing. Both kinds of dumps often contain sulfide minerals such as pyrite (FeS 2 ) or pyrrhotite (Fe 1Ϫx S, x ϭ 0 to 0.125) and release acidic metal-rich waters known as acid mine drainage (AMD)/acid rock drainage (ARD) because of chemical and microbial sulfide oxidation processes, e.g.,Over a period of several years, an oxidized zone with depleted sulfide content, low pH, and enrichment of secondary minerals develops above an unoxidized zone with unaltered material in the waste dump (e.g., references 4, 14, 21, 40, and 49).Several geomicrobiological investigations of sulfidic mine waste dumps located in different climate zones have been undertaken to gather information about microbial processes and diversity in these extreme environments. At such sites, aerobic, acidophilic, chemolithotrophic Bacteria or Archaea dissolve metal sulfides by oxidizing Fe(II) and sulfur compounds and generate AMD/ARD. Products resulting from these oxidation processes can be used by Fe(III)-and sulfate-reducing prokaryotes. When Fe(III) (hydr)oxides are dissolved, adsorbed or precipitated metals are released. Sulfate-reducing Bacteria or Archaea may also precipitate metals as metal sulfides (22,49,50).Primarily cultivation techniques have been used to enumerate prokaryotes involved in oxidation and reduction processes in sulfidic mine waste dumps (3,17,52,57,64). Cultivation techniques yield cell numbers merely according to physiological properties; therefore, only a subset of the whole microbial community is detected. Up to this point, only qualitative molecular biological tests were applied to sulfidic mine dumps such as cloning and subsequent sequencing (7), denaturing gradient gel electrophoresis (12, 38), and terminal restriction fragment length polymorphism (7, 13). In addition, protein and lipid analysis (37, 58) were performed with tailing samples. These investigations provided valuable information about the microbial diversity in mine dumps but little information about the quantities of the different microbial groups or species. The molecular biological quantification technique fluorescence in situ hybridization (FISH) and metagenomic an...

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“…Acidithiobacillus thiooxidans is an acidophilic, chemolithoautotrophic bacterium that oxidises reduced sulphur compounds and produces sulphuric acid [19,20]. For this reason, A. thiooxidans has been widely used in the bioleaching of low-grade and concentrates of sulphide minerals on both laboratory and industrial scales [21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Different strategies for recovering metals from CARON process residue

Cabrera

Gómez

Hernández

et al. 2011

Journal of Hazardous Materials

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a b s t r a c tThe capacity of Acidithiobacillus thiooxidans DMS 11478 to recover the heavy metals contained in the residue obtained from the CARON process has been evaluated. Different bioreactor configurations were studied: a two-stage batch system and two semi-continuous systems (stirred-tank reactor leaching and column leaching). In the two-stage system, 46.8% Co, 36.0% Mg, 26.3% Mn and 22.3% Ni were solubilised after 6 h of contact between the residue and the bacteria-free bioacid. The results obtained with the stirred-tank reactor and the column were similar: 50% of the Mg and Co and 40% of the Mn and Ni were solubilised after thirty one days. The operation in the column reactor allowed the solid-liquid ratio to be increased and the pH to be kept at low values (<1.0). Recirculation of the leachate in the column had a positive effect on metal removal; at sixty five days (optimum time) the solubilisation levels were as follows: 86% Co, 83% Mg, 72% Mn and Ni, 62% Fe and 23% Cr. The results corroborate the feasibility of the systems studied for the leaching of metals from CARON process residue and these methodologies can be considered viable for the recovery of valuable metals.

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Microorganisms Involved in Bioleaching and Nucleic Acid-Based Molecular Methods for Their Identification and Quantification

Cited by 105 publications

References 190 publications

Relationship between bioleaching performance, bacterial community structure and mineralogy in the bioleaching of a copper concentrate in stirred-tank reactors

Relationship between bioleaching performance, bacterial community structure and mineralogy in the bioleaching of a copper concentrate in stirred-tank reactors

Quantitative Microbial Community Analysis of Three Different Sulfidic Mine Tailing Dumps Generating Acid Mine Drainage

Different strategies for recovering metals from CARON process residue

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