Microorganisms Involved in Natural Fermentation of Asparagus cochinchinensis Roots and Changes in Efficacies after Fermentation

Abstract

The aim of this study was to examine the effect of Asparagus cochinchinensis (AC) and fermented AC (fAC) on microorganisms and efficacies. Methods: AC was fermented for four weeks without using any bacterial strains. Then we investigated fermentation characteristics including potential of hydrogen (pH), total sugar, microbial profiling and antioxidant compound contents such as total polyphenol and total flavonoid. The anti-obesity effects of AC and fAC were evaluated by using Oil Red O staining in 3T3-L1 adipocyte. Also anti-diabetic effects of them were evaluated by using 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-d-glucose (2-NBDG) uptake in C2C12 skeletal muscle cell. Results: Both pH and total sugar of fAC were decreased significantly compared to unfermented AC. And the abundance of total bacteria and lactic acid bacteria increased during fermentation, especially Lactobacillus plantarum. Also fermentation of AC increased the content of total polyphenol. On the metabolic aspects, we found that AC and fAC suppressed fat accumulation. Conclusions: After four weeks of fermentation, AC increased concentrations of active compounds, altered microbial composition, and inhibited fat accumulation such as triglyceride. These results indicate that fermentation of AC might be a beneficial therapeutic approach for obesity.

Analysis of Research Trends in Papers Published in the Journal of Korean Medicine for Obesity Research: Focused on 2010-2019

“…(2) In vitro 연구 50편의 전임상연구 중 in vitro 연구는 총 26편이었다. 실 험 대상에 따라 3T3-L1 세포를 이용한 경우 79,81,84,86,96,101,102) 와 C2C12 세포를 이용한 경우 83,85,87,89,92,96,99) 가 각각 7편…”

“…단미처방으로는 백출 80,82,85,86) 이 4회 사용되어 가장 빈 도가 높게 나타났으며 금은화 77,82,86) , 황련 77,82,86) 이 3회, 어 성초 82,86) , 황금 82,86) , 부자 89,94) 가 2회 사용되었다. 그 외 황 기 83) , 연잎-연근 혼합물 81) , 산사나무 유래물 87) , 육계 89) , 갈 근 89) , 백두옹 93) , 생강 94) , 커큐민 94) , 천문동 96) , 도라지 97) , 더 81,84,86,96,97,99,101,102) , 당대사와 에너지 대사에 대한 연구가 8편 82,83,85,89,92,95,96,102) , 항염증효과, 항산화효과, 유해균 억제효과 등 세포보호효과에 대한 연구가 총 16 편 [77][78][79][80]84,[86][87][88]90,…”